Determination of Cytotoxic and Apoptotic Properties of Lobaric Acid, a Secondary Metabolite of Lichen and Investigation of Its Theoretical Potential

Year 2024, Volume: 5 Issue: 3, 192 - 200, 30.09.2024

Hamit Emre Kızıl , Güleray Ağar , Yavuz Ekincioğlu

https://doi.org/10.56430/japro.1518450

Abstract

In this study, we aimed to elucidate some of the mechanisms of cell death induced by lobaric acid in A549 (human lung cancer) cells. For this purpose, the effects of cytotoxic concentrations on p53 and caspase-3 gene expressions were investigated. A549 cells were treated with varying concentrations of lobaric acid (12.5, 25, 50, and 100 µg/ml) for 48 hours and then their viability was evaluated and p53 and caspase-3 mRNA expressions were determined at statistically cytotoxic concentrations of 12.5, 50, and 100 µg/ml. According to beta-actin, it was determined that the increase in lobaric acid concentration revealed an upward trend in p53 and caspase-3 mRNA expressions. Furthermore, quantum chemical parameters such as frontier molecular orbitals, band gap energy and ionization potential, electronic affinity, chemical softness, chemical potential, electrophilicity index and chemical hardness were analyzed. Furthermore, molecular docking was performed to identify the binding sites and the binding behavior of lobaric acid to some target proteins (P53, Caspase-3 and Bcl-2).

Keywords

Apoptosis, Cytotoxicity, Lobaric acid, Molecular docking

Ethical Statement

The authors declare that this study does not require ethical committee approval or any legal permission. This study includes data obtained from a doctoral dissertation completed in 2016 (YÖK ID: 426087), and in line with the decisions taken by Ulakbim TR Dizin, Ethics Committee Permission is required for studies to be published since 2020. Other data obtained are web-based, virtual data.

Thanks

Some of the data in this study were obtained from Dr. Hamit Emre KIZIL’s PhD thesis (YÖK ID: 426087) completed in 2016. We are pleased to extend our deepest gratitude to the entire laboratory team for their unwavering moral support throughout the course of this doctoral thesis.

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