Impact of Mesorhizobium ciceri Inoculation on Symbiotic Nitrogen Fixation of Various Chickpea (Cicer arietinum L.) Cultivars

Year 2024, Volume: 5 Issue: 4, 255 - 264, 31.12.2024

Yasin Altuntaş , Erdal Elkoca , Selçuk Kodaz

https://doi.org/10.56430/japro.1565531

Abstract

In this study, conducted in pots under outdoor conditions, the aim was to determine the symbiotic performance of 10 registered chickpea cultivars (Akça, Aksu, Arda, Aslanbey, Bayram, Botan, Cevdetbey, Göktürk, Ilgaz and Yazıcı) inoculated with Mesorhizobium ciceri. Inoculation significantly increased the average nodule fresh weights, nodule dry weights, shoot height, shoot fresh weights, shoot dry weights, root fresh weights, root dry weights, chlorophyll content, N% and total N compared to the un-inoculated control. The shoot fresh weight, N% and total N significantly correlated with nodule weight. Additionally, chlorophyll content is highly correlated with N%. The magnitude of response to inoculation differed significantly among cultivars, except shoot height and root fresh weight. The amount of fixed N per plant by the cultivars varied significantly between 0 (Ilgaz) and 23.5 mg (Akça). Akça, Arda, Aslanbey, Cevdetbey, Göktürk and Yazıcı were the most compatible cultivars based on the amount of fixed N. These results show that inoculation efficiency and nitrogen fixation in chickpea can be increased by selecting compatible cultivars.

Keywords

Chickpea, Cultivar, Mesorhizobium ciceri, Symbiotic nitrogen fixation

Thanks

This article was prepared from Yasin Altuntaş's Master's Thesis.

References

• Beck, D. P. (1992). Yield and nitrogen fixation of chickpea cultivars in response to inoculation with selected rhizobial strains. Agronomy Journal, 84(3), 510-516. https://doi.org/10.2134/agronj1992.00021962008400030029x
• Bhuiyan, M. A. H., Khanam, D., Ullah, M. H., & Alam, M. M. (2009). Effect of inoculation and varietal interactions of chickpea at southern region of Bangladesh. Bulletin of the Institute Tropical Agriculture, Kyushu University, 32(1), 17-23. https://doi.org/10.11189/bita.32.17
• Bidlack, J. E., Rao, S. C., & Demezas, D. H. (2001). Nodulation, nitrogenase activity, and dry weight of chickpea and pigeon pea cultivars using different Bradyrhizobium strains. Journal of Plant Nutrition, 24(3), 549-560. https://doi.org/10.1081/PLN-100104979
• Bohlool, B., Ladha, J. K., Garrity, D. P., & George, T. (1992). Biological nitrogen fixation for sustainable agriculture: A perspective. Plant and Soil, 141, 1-11. https://doi.org/10.1007/BF00011307
• Bordeleau, L. M., & Prevost, D. (1994). Nodulation and nitrogen fixation in extreme environments. Plant and Soil, 161, 115-125. https://doi.org/10.1007/BF02183092
• Buttery, B. R., Park, S-J., & van Berkum, P. (1997). Effects of common bean (Phaseolus vulgaris L.) cultivar and rhizobium strain on plant growth, seed yield and nitrogen content. Canadian Journal of Plant Science, 77, 347-351. https://doi.org/10.4141/P96-088
• Danso, S. K. A., Hera, C., & Douka, C. (1987). Nitrogen fixation in soybean as influenced by cultivar and Rhizobium strain. Plant and Soil, 99, 163-174. https://doi.org/10.1007/BF02370163
• Davis, T. M., Foster, K. W., & Phillips, D. A. (1985). Nodulation mutants in chickpea. Crop Science, 25(2), 345-348. https://doi.org/10.2135/cropsci1985.0011183X002500020033x
• Di Bonito, R., Porreca, G., & Mannelli, S. (1990). Competition for nodulation of Rhizobium spp. (Cicer arietinum L.) inoculated on different cultivars of chickpea. Symbiosis, 9(1-3), 405-409.
• Dwivedi, S. L., Sahrawat, K. L., Upadhyaya, H. D., Mengoni, A., Galardini, M., Bazzicalupo, M., Biondi, E. G., Hungria, M., Kaschuk, G., Blair, M. W., & Ortiz, R. (2015). Advances in host plant and Rhizobium genomics to enhance symbiotic nitrogen fixation in grain legumes. Advances in Agronomy, 129, 1-116. https://doi.org/10.1016/bs.agron.2014.09.001
• El Hadi, E. A., & Elsheikh, E. A. E. (1999). Effect of Rhizobium inoculation and nitrogen fertilization on yield and protein content of six chickpea (Cicer arietinum L.) cultivars in marginal soils under irrigation. Nutrient Cycling in Agroecosystems, 54, 57-63. https://doi.org/10.1023/A:1009778727102
• Elkoca, E., Kantar, F., & Sahin, F. (2008). Influence of nitrogen fixing and phosphorus solubilizing bacteria on the nodulation, plant growth, and yield of chickpea. Journal of Plant Nutrition, 31, 157-171. https://doi.org/10.1080/01904160701742097
• Elkoca, E., Turan, M., & Dönmez, M. F. (2010). Effects of single, dual and triple inoculations with Bacillus subtilis, Bacillus megaterium and Rhizobium leguminosarum bv. phaseoli on nodulation, nutrient uptake, yield and yield parameters of common bean (Phaseolus vulgaris L. cv. ‘Elkoca-05’). Journal of Plant Nutrition, 33, 2104-2119. https://doi.org/10.1080/01904167.2010.519084
• Elkoca, E., Kocli, T., Gunes, A., & Turan, M. (2015). The symbiotic performance and plant nutrient uptake of certain nationally registered chickpea (Cicer arietinum L.) cultivars of Turkey. Journal of Plant Nutrition, 38, 1427-1443. https://doi.org/10.1080/01904167.2014.983123
• Eusuf Zai, A. K., Solaiman, A. R. M., & Ahmed, J. U. (1999). Response of some chickpea varieties to Rhizobium inoculation in respect to nodulation, biological nitrogen fixation and dry matter yield. Bangladesh Journal of Microbiology, 16(2), 135-144.
• Fageria, N. K., & Baligar, V. C. (2005). Enhancing nitrogen use efficiency in crop plants. Advances in Agronomy, 88, 97-185. https://doi.org/10.1016/S0065-2113(05)88004-6
• FAO. (2022). Crops and livestock products. Retrieved Jun 27, 2024, from https://www.fao.org/faostat/en/#data/QCL
• Farid, M., & Navabi, A. (2015). N2 fixation ability of different dry bean genotypes. Canadian Journal of Plant Science, 95, 1243-1257. https://doi.org/10.4141/cjps-2015-084
• Goyal, R. K., Mattoo, A. K., & Schmidt, M. A. (2021). Rhizobial–host interactions and symbiotic nitrogen fixation in legume crops toward agriculture sustainability. Frontiers in Microbiology, 12, 669404. https://doi.org/10.3389/fmicb.2021.669404
• Gul, R., Khan, H., Khan, N. U., & Khan, F. U. (2014). Characterization of chickpea germplasm for nodulation and effect of rhizobium inoculation on nodules number and seed yield. The Journal of Animal & Plant Sciences, 24(5), 1421-1429.
• Hardarson, G., & Danso, S. K. A. (1993). Methods for measuring biological nitrogen fixation in grain legumes. Plant and Soil, 152, 19-23. https://doi.org/10.1007/BF00016330
• Hoque, M. F., Sattar, M. A., & Dutta, R. K. (1999). Nodulation, chlorophyll and leghaemoglobin content of soybean as affected by Bradyrhizobium japonicum and sulphur molybdenum supplementation. Bangladesh Journal of Botany, 28(1), 47-52.
• Hossain, Z., Wang, X., Hamel, C., & Gan, Y. (2018). Nodulation and nitrogen accumulation in pulses vary with species, cultivars, growth stages, and environments. Canadian Journal of Plant Science, 98(3), 527-542. https://doi.org/10.1139/cjps-2017-0114
• Hungria, M., & Bohrer, T. R. J. (2000). Variability of nodulation and dinitrogen fixation capacity among soybean cultivar. Biology and Fertility of Soils, 31, 45-52. https://doi.org/10.1007/s003740050622
• Kantar, F., Hafeez, F. Y., Shivakumar, B. G., Sundaram, S. P., Tejera, N. A., Aslam, A., Bano, A., & Raja, P. (2007). Chickpea: Rhizobium management and nitrogen fixation. In S. S. Yadav, R. J. Redden, W. Chen & B. Sharma (Eds.), Chickpea breeding and management (pp. 179-192). CAB International. https://doi.org/10.1079/9781845932138.008
• Keneni, G., Bekele, E., Assefa, F., Imtiaz, M., Debele, T., Dagne, K., & Getu, E. (2012). Phenotypic diversity for symbio-agronomic characters in Ethiopian chickpea (Cicer arietinum L.) germplasm accessions. African Journal of Biotechnology, 11(63), 12634-12651. https://doi.org/10.5897/AJB12.428
• Kipe-Nolt, J. A., & Giller, K. E. (1993). A field evaluation using the 15N isotope dilution method of lines of Phaseolus vulgaris L. bred for increased nitrogen fixation. Plant and Soil, 152, 107-114. https://doi.org/10.1007/BF00016339
• Kumawat, S. M., Dhakar, L. L., & Maliwal, P. L. (2000). Effect of irrigation regimes and nitrogen on yield, oil content and nutrient uptake of soybean (Glycine max). Indian Journal of Agronomy, 45(2), 361-366.
• Li, S. X., Wang, Z. H., & Stewart, B. A. (2013). Responses of crop plants to ammonium and nitrate N. Advances in Agronomy, 118, 205-397. https://doi.org/10.1016/B978-0-12-405942-9.00005-0
• Mendoza-Tafolla, R. O., Juarez-Lopez, P., Ontiveros-Capurata, R. E., Sandoval-Villa, M., Iran, A. T., & Alejo-Santiago, G. (2019). Estimating nitrogen and chlorophyll status of romaine lettuce using SPAD and at LEAF Readings. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(3), 751-756. https://doi.org/10.15835/nbha47311525
• Mohammadi, K., & Sohrabi, Y. (2012). Bacterial biofertilizers for sustainable crop production: A review. Journal of Agricultural and Biological Science, 7(5), 307-316.
• Ndiaye, M. A. F., Spencer, M. M., & Gueye, M. (2000). Genetic variability in dinitrogen fixation between cowpea [Vigna unguiculata (L.) Walp] cultivars determined using the nitrogen-15 isotope dilution technique. Biology and Fertility of Soils, 32, 318-320. https://doi.org/10.1007/s003740000254
• Odabaş, M. S., & Gülümser, A. (2001). An investigation on the effects of yield, yield components and chlorophyll amounts of the leaves of different nitrogen sources in bean (Phaseolus vulgaris L.). Anadolu Tarım Bilimleri Dergisi, 16(1), 42-47.
• Öğütçü, H., Algur, O. F., Elkoca, E., & Kantar, F. (2008). The determination of symbiotic effectiveness of Rhizobium strains isolated from wild chickpeas collected from high altitudes in Erzurum. Turkish Journal of Agriculture and Forestry, 32, 241-248.
• Öğütçü, H., Kasımoğlu, C., & Elkoca, E. (2010). Effects of rhizobium strains isolated from wild chickpeas on the growth and symbiotic performance of chickpeas (Cicer arietinum L.) under salt stress. Turkish Journal of Agriculture and Forestry, 34, 361-371. https://doi.org/10.3906/tar-0904-34
• Pandey, R. P., Srivastava, A. K., Gupta, V. K., O’Donovan, A., & Ramteke, P. W. (2018). Enhanced yield of diverse varieties of chickpea (Cicer arietinum L.) by different isolates of Mesorhizobium ciceri. Environmental Sustainability, 1, 425-435. https://doi.org/10.1007/s42398-018-00039-9
• Perret, X., Staehelin, C., & Broughton, W. J. (2000). Molecular basis of symbiotic promiscuity. Microbiology and Molecular Biology Reviews, 64, 180-201. https://doi.org/10.1128/mmbr.64.1.180-201.2000
• Priyadarsini, L., Singh, P. K., Chatterjee, C., Sadhukhan, R., & Biswas, T. (2017). Estimation of genetic variability of nodulation characters and their association with different agromorphic characters and yield in chickpea (C. arietinum L.). International Journal of Current Microbiology and Applied Sciences, 6(9), 1928-1935. https://doi.org/10.20546/ijcmas.2017.609.236
• Roy, A., Ghosh, S., & Kundagrami, S. (2018). Nodulation pattern and its association with seed yield in chickpea (Cicer arietinum L.) germplasms. Indian Journal of Agricultural Research, 53(2), 172-177. https://doi.org/10.18805/IJARe.A-5162
• Rupela, O. P., & Saxena, M. C. (1987). Nodulation and nitrogen fixation in chickpea. In M. C. Saxena & K. B. Singh (Eds.), The chickpea (pp. 191-206). CAB International.
• Santalla, M., Amurrio, J. M., Ron, J. M., & De, A. M. (2001). Symbiotic interactions between Rhizobium leguminosarum strains and elite cultivars of Pisum sativum L. Journal of Agronomy and Crop Science, 187(1), 59-68. https://doi.org/10.1046/j.1439-037X.2001.00502.x
• Solaiman, A. R. M., Hossain, D., & Rabbani, M. G. (2007). Influence of Rhizobium inoculant and mineral nitrogen on some chickpea varieties. Bangladesh Journal of Microbiology, 24(2), 146-150. https://doi.org/10.3329/bjm.v24i2.1261
• Solaiman, A. R. M., Talukder, M. S., & Rabbani, M. G. (2010). Influence of some Rhizobium strains on chickpea: Nodulation, dry matter yield and nitrogen uptake. Bangladesh Journal of Microbiology, 27(2), 61-64. https://doi.org/10.3329/bjm.v27i2.9174
• Somasegaran, P., Hoben, H. J., & Gurgun, V. (1988). Effects of inoculation rate, rhizobial competition, and nitrogen fixation in chickpea. Agronomy Journal, 80(1), 68-73. https://doi.org/10.2134/agronj1988.00021962008000010016x
• Verma, D. P. S. (1984). Involvement of specific host genes in symbiotic nitrogen fixation. In V. L. Chopra, B. C. Joshi, R. P. Sharma & H. C. Bansal (Eds.), Genetics: New frontiers, volume II recombinant DNA technology (pp. 227-239). Oxford & IBH Publishing Co.
• Verma, J. P., Yadav, J., Tiwari, K. N., & Kumar, A. (2013). Effect of indigenous Mesorhizobium sp. and plant growth promoting rhizobacteria on yield and nutrient uptake of chickpea (Cicer arietinum L.). Ecological Engineering, 51, 282-286. https://doi.org/10.1016/j.ecoleng.2012.12.022
• Wang, Q., Liu, J., & Zhu, H. (2018). Genetic and molecular mechanisms underlying symbiotic specificity in legume-rhizobium interactions. Frontiers in Plant Science, 9, 313. https://doi.org/10.3389/fpls.2018.00313
• Wood, C. W., Reeves, D. W., Duffield, R. R., & Edmisten, K. L. (1992). Field chlorophyll measurements for evaluation of corn nitrogen status. Journal of Plant Nutrition, 15(4), 487-500. https://doi.org/10.1080/01904169209364335