The Characterization of Prokaryotic Diversity in Lake Beyşehir Using a 16s Metagenomics Study

Year 2023, , 719 - 729, 20.09.2023

Fahri Pat Sultan Fidan Pedük Neşe Akçay Hatice Kübra Kızıl Pat Ercan Arıcan

https://doi.org/10.28979/jarnas.1217912

Abstract

Lake Beysehir, located in the Central Anatolia Region, is the biggest lake in Central Anatolia, providing drinking water and irrigation. It is Turkey's third-largest lake and the largest freshwater lake. Its waters are fresh water and its depth is around 8-9 m at most. The deepest point is mentioned as 12, 14, and 15 m in different sources. Although there is a lake used by such a large population in our country, there are not enough studies on microbial diversity, so revealing the diversity is important for the lake and the population around the lake.
Next-generation sequencing (NGS) research has made it possible to sequence RNA and DNA considerably quicker and cheaply than in by assessing the PCR amplicon, we utilized NGS high-throughput methods for the metagenomic research of Lake Beyşehir sequences of 16S rDNA (regions V3–V4). The analyzes revealed the phylum Proteobacteria, Verrucomicrobia, Bacteroidetes and Actinobacteria.

Keywords

Metagenomic, NGS, 16S rRNA, Ecology, Microbial diversity

Supporting Institution

İstanbul Üniversitesi Bilimsel Araştırma Projeleri Birimi

Project Number

FDK-2019-34349

Thanks

The laboratory studies of this study were carried out in the molecular biology and genetics laboratories of Istanbul University, Faculty of Science. Endless thanks to KOSKİ for providing biochemical measurements of Beyşehir lake, which is our study area. The Scientific Research Projects Coordination Unit of Istanbul University financed this study.

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