Anticancer, Antioxidant, Antimicrobial Activities, and HPLC Analysis of Alcoholic Extracts of Parthenocissus quinquefolia L. Plant Collected from Çanakkale

Year 2024, , 116 - 133, 15.03.2024

Ferah Cömert Önder , Sevil Kalın , Özlem Maraba , Alper Önder , Pınar Ilgın , Ersin Karabacak

https://doi.org/10.28979/jarnas.1377901

Cited By: 1

Abstract

This study aimed to contribute to revealing the health effects of the Parthenocissus quinquefolia L. (PQ) plant by examining the biological activities of various extracts of the plant. An ethnobotanical important medicinal plant, PQ belongs to the Vine family (Vitaceae) and is known for its medicinal uses. Herein, fruit and leaf alcoholic extracts of the plant exhibited anticancer activity on triple negative breast cancer (TNBC) cell lines, estrogen receptor positive (ER+) (MCF-7), and human colon adenocarcinoma (HT-29) cancer cell lines. Furthermore, fruit methanol extracts displayed strong radical scavenging activity with low IC50 values such as 0.51 mg/mL, 0.80 mg/mL, 0.84 mg/mL, and 1.45 mg/mL. The highest TEAC values (18.72 mM Trolox/mg, 9.90 mM Trolox/mg, and 8.41 mM Trolox/mg) were calculated for the extracts of red leaves. The antifungal activity was determined against Candida albicans as fungal pathogen. The cell apoptosis with methanol extract was obtained between 20 µg/mL and 70 µg/mL for 48 h and 72 h treatments by Hoechst 33258 staining of nuclei under a fluorescence microscopy. HPLC analysis was performed for methanol extracts to determine major bioactive components. Consequently, this plant extracts may guide to new strategies and may be uses in medicinal applications for further investigations.

Keywords

Anticancer, Antimicrobial, Antioxidant, HPLC, Parthenocissus quinquefolia L.

Supporting Institution

This study was supported by Çanakkale Onsekiz Mart University, The Scientific Research Coordina-tion Unit

Project Number

THD-2022-4041

Thanks

We thank Prof. Dr. Mehmet Ay for laboratory facilities, Prof. Dr. Hava Özay for UV-Vis spectropho-tometre, Prof. Dr. Zuhal Hamurcu for breast cancer cell lines, Prof. Dr. Tuğba Tümer for colon cancer cell line, Çanakkale Onsekiz Mart University Experimental Research Application and Research Center for laboratory facilities, and Iğdır University Research Laboratory Practice and Research Center (AL-UM) for HPLC analysis. The microorganisms were obtained from the microbiology laboratory of the Bioengineering faculty of Çanakkale Onsekiz Mart University.

References

• O. S. Nwozo, E. M. Effionga, P. M. Aja, C. G. Awuchi, Antioxidant, phytochemical, and therapeutic properties of medicinal plants: A review, International Journal of Food Properties 26 (1) (2022) 359–388.
• M. Samtiya, R. E. Aluko, T. Dhewa, J. M. Moreno-Rojas, Potential health benefits of plant food-derived bioactive components: An overview, Foods 10 (4) (2021) 839 25 pages.
• M. Khalid, M. Amayreh, S. Sanduka, Z. Salah, F. Al-Rimawi, G. M. Al-Mazaideh, A. A. Alanezi, F. Wedian, F. Alasmari, M. H. Faris Shalayel, Assessment of antioxidant, antimicrobial, and anticancer activities of Sisymbrium officinale plant extract, Heliyon 8 (9) (2022) e10477 6 pages.
• A. S. Choudhari, P. C. Mandave, M. Deshpande, P. Ranjekar, O. Prakash, Phytochemicals in cancer treatment: From preclinical studies to clinical practice, Frontiers in Pharmacology 10 (2020) 1614 17 pages.
• N. H. Nguyen, Q. Ta, Q. T. Pham, T. Luong, V. T. Phung, T. H. Duong, V. G. Vo, Anticancer activity of novel plant extracts and compounds from Adenosma bracteosum (Bonati) in human lung and liver cancer cells, Molecules 25 (12) (2020) 2912 16 pages.
• P. Chaudhary, P. Janmeda, A. O. Docea, B. Yeskaliyeva, A. F. Abdull Razis, B. Modu, D. Calina, J. Sharifi-Rad, Oxidative stress, free radicals and antioxidants: potential crosstalk in the pathophysiology of human diseases, Frontiers in Chemistry 11 (2023) 1158198 24 pages.
• M. Michalak, Plant-derived antioxidants: Significance in skin health and the ageing process, International Journal of Molecular Sciences 23 (2) (2022) 585 29 pages.
• L. Shi, W. Zhao, Z. Yang, V. Subbiah, H. A. R. Suleria, Extraction and characterization of phenolic compounds and their potential antioxidant activities, Environmental Science and Pollution Research 29 (54) (2022) 81112–81129.
• Y. Kocak, F. Altindag, E. Okumus, G. Oto, M. Hafif Bayir, S. Alparslan, The effect of quercetin against oxidative stress and possible histopathological changes due to Bisphenol-A exposure in rat ovary, Chronicles of Precision Medical Researchers 4 (3) (2023) 290–296.
• F. Summiya, P. Anjum, K. Zaheer, S. Andleeb, S. Shabnum, M. Asma, Phytochemical screening and antioxidant potential of Parthenocissus quinquefolia [L.] planch extracts of bark and stem, Pakistan Journal of Pharmaceutical Sciences 31 (5) (2018) 1813-1816
• K. Jomova, R. Raptova, S. Y. Alomar, S. H. Alwasel, E. Nepovimova, K. Kuca, M. Valko, Reactive oxygen species, toxicity, oxidative stress, and antioxidants: Chronic diseases and aging, Archives of Toxicology 97 (10) (2023) 2499–2574.
• G. S. Ćetković, J. M. Čanadanović-Brunet, S. M. Djilas, V. T. Tumbas, S. L. Markov, D. D. Cvetković, Antioxidant potential, lipid peroxidation inhibition and antimicrobial activities of Satureja montana L. subsp. kitaibelii extracts, International Journal of Molecular Sciences 8 (10) (2007) 1013–1027.
• Z.-Ud-D. Khan, S. Faisal, A. Perveen, A. A. Sardar, S. Z. Siddiqui, Phytochemical properties and antioxidant activities of leaves and fruits extracts of Parthenocissus Quinquefolia (L.) planch, Bangladesh Journal of Botany 47 (1) (2018) 33–38.
• A. A. Mohamed, M. M. Salah, M. M. Z. El-Dein, M. EL-Hefny, H. M. Ali, D. A. A. Farraj, A. A. Hatamleh, M. Z. M. Salem, N. A. Ashmawy, Ecofriendly bioagents, Parthenocissus quinquefolia, and Plectranthus neochilus extracts to control the early blight pathogen (Alternaria solani) in tomato, Agronomy 11 (5) (2021) 911 17 pages.
• A. Zardi-Bergaouia, M. Jouinia, M. Znatia, A. El Ayeb-Zakhamab, H. B. Janneta, Physico-chemical properties, composition and antioxidant activity of seed oil from the Tunisian Virginia Creeper (Parthenocissus quinquefolia (L.) planch), Journal of the Tunisian Chemical Society 18 (2016) 89–95.
• K. Zaheer-Ud-Din, F. Summiya, P. Anjum, A. A. Sardar, S. Z. Siddiqui, Phytochemical properties and antioxidant activities of leaves and fruits extracts of Parthenocissus quinquefolia (L.) Planch, Bangladesh Journal of Botany 47 (1) (2018) 33–38.
• M. B. Ticha, N. Meksi, H. E. Attia, W. Haddar, A. Guesmi, H. B. Jannet, M. F. Mhenni, Ultrasonic extraction of Parthenocissus quinquefolia colorants: Extract identification by HPLC-MS analysis and cleaner application on the phytodyeing of natural fibres, Dyes and Pigments 141 (2017) 103–111.
• A. G. Atanasov, B. Waltenberger, E. M. Pferschy-Wenzig, T. Linder, C. Wawrosch, P. Uhrin, V. Temml, L. Wang, S. Schwaiger, E. H. Heiss, J. M. Rollinger, D. Schuster, J. M. Breuss, V. Bochkov, M. D. Mihovilovic, B. Kopp, R. Bauer, V. M. Dirsch, H. Stuppner, Discovery and resupply of pharmacologically active plant-derived natural products: A review, Biotechnology Advances 33 (8) (2015) 1582–1614.
• Kivanç, M. R., Önder, A., Cömert Önder, F., Ilgin, P. (2023). Pektin/p(HEMA-ko-AAc) Hidrojellerine Gömülü Gümüş Nanopartiküllerin Yeşil Sentez Yöntemi olarak Parthenocissus quinquefolia L. Bitki Özütü ile Üretilmesi. Lapseki Meslek Yüksekokulu Uygulamalı Araştırmalar Dergisi, 4(7), 12-19. https://dergipark.org.tr/tr/pub/ljar/issue/78716/1290920
• F. Comert Onder, M. Ay, S. D. Sarker, Comparative study of antioxidant properties and total phenolic content of the extracts of Humulus lupulus L. and quantification of bioactive components by LC–MS/MS and GC–MS, Journal of Agricultural and Food Chemistry 61 (44) (2013) 10498–10506.
• F. Comert Onder, S. Kalin, A. Onder, H. Ozay, O. Ozay, Assessment of release and anticancer effect of innovative pH-responsive and antioxidant-biodegradable hydrogel by using Parthenocissus quinquefolia L. extract as a crosslinker, Journal of Drug Delivery Science and Technology 90 (5) (2023) 105113 12 pages.
• F. Ozsoy, F. Comert Onder, P. Ilgin, H. Ozay, A. Onder, O. Ozay, Self-assembled silibinin-containing phosphazene/cystamine hybrid nanospheres as biodagradable dual-drug carriers with improved anticancer activity on a breast cancer cell line, Materials Today Communications 37 (2023) 107116 11 pages.
• I. Fatima, N. Safdar, W. Akhtar, A. Munir, S. Saqib, A. Ayaz, S. Bahadur, A. F. Alrefaei, F. Ullah, W. Zaman, Evaluation of potential inhibitory effects on acetylcholinesterase, pancreatic lipase, and cancer cell lines using raw leaves extracts of three fabaceae species, Heliyon 9 (5) (2023) e15909 8 pages.
• R. Delalat, S. A. Sadat Shandiz, B. Pakpour, Antineoplastic effectiveness of silver nanoparticles synthesized from Onopordum acanthium L. extract (AgNPs-OAL) toward MDA-MB231 breast cancer cells, Molecular Biology Reports 49 (2022) 1113–1120.
• Y. Kocak, G. Oto, I. Meydan, H. Seckin, Van bölgesinde yetişen Allium schoenoprasum L. bitkisinin toplam flavonoid, DPPH radikal söndürme, lipid peroksidasyonu ve antimikrobiyal aktivitesinin araştırılması, Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 30 (1) (2020) 147–155.
• S. Durmuş, B. Yilmaz, M. R. Kıvanç, A. Onder, P. Ilgin, H. Ozay, O. Ozay, Synthesis, characterization, and in vitro drug release properties of AuNPs/p (AETAC-co-VI)/Q nanocomposite hydrogels, Gold Bulletin 54 (2021) 75–87.
• N. Partovi, H. H. Kumleh, E. Mirzajani, M. Farhadpour, Identification of Lilium ledebourii antiproliferative compounds against skin, bone, and oral cancer cells, Avicenna Journal of Phytomedicine 13 (6) (2023) 626–640.
• T. Kundakovıć, T. Stanojkovıć, M. Mılenkovıć, J. Grubın, Z. Juranıć, B. Stevanovıć, N. Kovačevıć, Cytotoxic, antioxidant, and antimicrobial activities of Ampelopsis brevipedunculataand Parthenocissus tricuspidate (VITACEAE), Archives of Biological Sciences Belgrade 60 (4) (2018) 641–647.
• A. Elfadadny, R. F. Ragab, R. Hamada, S. K. Al Jaouni, J. Fu, S. A. Mousa, H. Ali, Natural bioactive compounds-doxorubicin combinations targeting topoisomerase II-alpha: Anticancer efficacy and safety, Toxicology and Applied Pharmacology 461 (2023) 116405 18 pages.
• S. Saharkhiz, A. Zarepour, N. Nasri, M. Cordani, A. Zarrabi, A comparison study between doxorubicin and curcumin co-administration and co-loading in a smart niosomal formulation for MCF-7 breast cancer therapy, European Journal of Pharmaceutical Sciences 191 (2023) 106600 10 pages.
• M. Sudhakaran, M. R. Parra, H. Stoub, K. A. Gallo, A. I. Doseff, Apigenin by targeting hnRNPA2 sensitizes triple-negative breast cancer spheroids to doxorubicininduced apoptosis and regulates expression of ABCC4 and ABCG2 drug efflux transporters, Biochemical Pharmacology 182 (2020) 114259 13 pages.
• B. Pateliya, V. Burade, S. Goswami, Combining naringenin and metformin with doxorubicin enhances anticancer activity against triple-negative breast cancer in vitro and in vivo, European Journal of Pharmacology 891 (2021) 173725 10 pages.
• H. M. Shrirang, B. J. Kariyil, R. John, Anticancer potential of methanol extract of seeds of Artocarpus hirsutus in human breast cancer cell lines, Indian Journal of Traditional Knowledge 22 (2) (2023) 390– 400.
• N. Rattanata, S. Daduang, S. Phaetchanla, W. Bunyatratchata, B. Promraksa, R. Tavichakorntrakool, P.Uthaiwat, P. Boonsiri, J. Daduang, Antioxidant and antibacterial properties of selected Thai weed extracts, Asian Pacific Journal of Tropical Biomedicine 4 (11) (2014) 890–895.
• S. Faisal, A. Perveen, Z. U. Khan, A. A. Sardar, S. Shaheen, A. Manzoor, Phytochemical screening and antioxidant potential of Parthenocissus quinquefolia (L.) planch extracts of bark and stem, Pakistan Journal of Pharmaceutical Sciences 31 (5) (2018) 1813–1816.
• N. R. Ismail, E. J. Kadhim, Phytochemical screening and isolation of new compounds, International Journal of Drug Delivery Technology 11 (3) (2021) 1033–1039.
• J. Dai, R. J. Mumper, Plant phenolics: extraction, analysis and their antioxidant and anticancer properties, Molecules 15 (10) (2010) 7313–7352.
• H. Hajimehdipoor, R. Shahrestani, M. Shekarchi, Investigating the synergistic antioxidant effects of some flavonoid and phenolic compounds, Research Journal of Pharmacognosy 1 (3) (2014) 35–40.
• A. Oreopoulou, D. Tsimogiannis, V. Oreopoulou, Extraction of polyphenols from aromatic and medicinal plants: an overview of the methods and the effect of extraction parameters, in: R. R. Watson (Ed.), Polyphenols in Plants: Isolation, Purification and Extract Preparation, 2nd Edition, Academic Press, Cambridge, 2019, Ch. 15, pp. 243–259.
• A. Altemimi, N. Lakhssassi, A. Baharlouei, D. G. Watson, D. A. Lightfoot, Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts, Plants 6 (4) (2017) 42 23 pages.
• P. Anokwuru, G. N. Anyasor, O. Ajibaye, O. Fakoya, P. Okebugwu, Effect of extraction solvents on phenolic, flavonoid and antioxidant activities of three nigerian medicinal plants, Nature and Science 9 (7) (2011) 53–61.
• D. Stalikas, Extraction, separation, and detection methods for phenolic acids and flavonoids, Journal of Separation Science 30 (18) (2007) 3268–3295.
• M. Kisiriko, M. Anastasiadi, L. A. Terry, A. Yasri, M. H. Beale, J. L. Ward, Phenolics from medicinal and aromatic plants: Characterisation and potential as biostimulants and bioprotectants, Molecules 26 (21) (2021) 6343 37 pages.
• G. Pizzino, N. Irrera, M. Cucinotta, G. Pallio, F. Mannino, V. Arcoraci, F. Squadrito, D. Altavilla, A. Bitto, Oxidative stress: Harms and benefits for human health, Oxidative Medicine and Cellular Longevity 2017 (2017) Article ID 8416763 13 pages.
• Clinical and Laboratory Standards Institute, Laboratory documents: Development and control; Approved guideline, 5th Edition, Clinical and Laboratory Standards Institute, 2006.
• H. Hamrouni, K. B. Othman, H. Benmoussa, S. Idoudi, H. Najjaa, M. Neffati, W. Elfalleh, Phenolic profiling, antioxidant, and antibacterial activities of selected medicinal plants from Tunisia, Chemistry & Biodiversity 20 (8) (2023) e202300265 10 pages.
• S. Dubale, D. Kebebe, A. Zeynudin, N. Abdissa, S. Suleman, Phytochemical screening and antimicrobial activity evaluation of selected medicinal plants in Ethiopia, Journal of Experimental Pharmacology 15 (2023) 51–62.
• T. T. Cushnie, A. J. Lamb, Recent advances in understanding the antibacterial properties of flavonoids, International Journal of Antimicrobial Agents 38 (2) (2011) 99–107.