Monitoring the Effect of Molecular Weight and Blend Ratio of Polyelectrolytes on the Development of Multilayer Films and Their Use in Drug Adsorption/Desorption Applications by QCM-D Technique

Year 2024, , 190 - 206, 15.03.2024

Dilara Yılmaz Aykut , Öznur Yolaçan , Hüseyin Deligöz

https://doi.org/10.28979/jarnas.1407665

Abstract

In this study, the preparation of multilayered functional films with controllable thickness composition and morphology using the Layer-by-Layer (LbL) technique from weak and strong polyelectrolyte couples and the use of dairy in the release of different structured drugs have been studied. Besides, following the growth of self-assembled pure/blend LbL thin films at the nanogram level, intraction with the model drug LbL film surfaces in drug loading and release characterizations and viscoelastic changes of films intructed with drugs have been successfully investigated. Moreover, UV-Vis and QCM-D analyses have been performed to follow the growth of LbL functional thin films and to find polyelectrolyte ratios in film structure. In addition, scanning electron microscopy (SEM) and contact angle measurements have been carried out to investigate the controllable surface of LbL pure/blend structured thin films depending on the use of biodegradable and synthetic polyelectrolyte couples, blend ratio, and LbL deposition conditions. Subsequently, drug loading and release stabilities for the ibuprofen sodium drug have been performed using QCM-D and UV-Visible at neutral (pH: 6.8) acidic (pH: 2) and basic (pH: 11) PBS solutions. In addition to offering an innovative, economic, and practical approach, new drug release platforms with (i) surfaces of controllable morphology and composition, (ii) adjustable loading and release properties for model drugs, and (iii) sustuinable drug release in wide range of pH: 2-11, have been developed. The obtained results show that LbL functional films must have different properties considering the surface properties of the film, the pH of the media, the interaction between drug and film for drugs, and their applications. In this respect, the preparation of functional films with controllable proporties using the LbL technique and their use in drug release applications exhibit quite promising results.

Keywords

Ibuprofen adsorption/desorption, thin films, polyelectrolyte multilayers, QCM-D, Layer-by-Layer (LbL)

Supporting Institution

TÜBİTAK

Project Number

112M290

Thanks

Tüm yazarlar, 112M290 numaralı proje kapsamında Türkiye Bilimsel ve Teknolojik Araştırma Kurumu'nun (TÜBİTAK) finansal desteğine teşekkür etmektedir.

Polielektrolitlerin Molekül Ağırlığı ve Harman Oranının Çok Tabakalı Filmlerin Gelişimine Etkisinin ve İlaç Adsorpsiyon/Desorpsiyon Uygulamalarında Kullanımlarının QCM-D Tekniğiyle Takibi

Abstract

Bu çalışmada, harman zayıf ve kuvvetli polielektrolit çiftlerinin kullanımı ile tabakalı kaplama, layer-by-layer (LbL), yöntemi ile çok tabakalı fonksiyonel ince filmlerin kontrol edilebilir kalınlıkta, kompozisyonda, morfolojide hazırlanması ve ilaç salımında kullanımları araştırılmıştır. Kuartz Kristal Mikrobalans-Dissipasyon (QCM-D) tekniği ile kendi kendine bir araya gelen saf/harman yapılı LbL ince filmlerin gelişimlerinin nanogram seviyesinde takibi, ilaç yükleme/salım karakterizasyonu, model ilaç ile LbL film yüzeyi arası etkileşimler ve filmlerin ilaçla etkileşimde viskoelastik değişimleri başarıyla incelenmiştir. Ayrıca, hazırlanan çok tabakalı harman fonksiyonel ince filmlerin gelişiminin takibi için UV ve QCM-D analizi gerçekleştirilerek harman film yapısındaki polielektrolit oranları tespit edilmiştir. Ek olarak, LbL saf/harman yapılı ince filmlerin kontrol edilebilir yüzey ve topografik özelliklerinin kullanılan biyobozunur ve sentetik polielektrolit çiftlerine, harman oranına ve LbL kaplama koşullarına bağlı olarak değişimlerinin incelenmesi amacıyla taramalı elektron mikroskopu (SEM) analizleri ve yüzey temas açısı çalışmaları gerçekleştirilmiştir. Hazırlanan LbL saf/harman yapılı fonksiyonel filmlerde ibuprofen sodyum ilacı için yükleme ve salım çalışmaları QCM-D tekniği ve UV-Vis analizleriyle nötral (pH:6,8), asidik (pH:2) ve bazik (pH:11) fosfat tampon çözeltisinde (PBS) gerçekleştirilmiştir. Çalışmada, ilaç salım sistemleri için yenilikçi, ekonomik ve pratik bir yaklaşımın sunulmasının yanı sıra; LbL kaplama koşullarına bağlı olarak (i) morfolojisi ve kompozisyonu kontrol edilebilen, (ii) ilaç yükleme ve salım özellikleri ayarlanabilen ve geniş bir pH (pH:2-11) aralığında ilaç salımı yapabilen sistemler geliştirilmiştir. Elde edilen sonuçlar, ilaç uygulamaları için LbL fonksiyonel filmlerin yapısına, yüzey özelliklerine, ortamın pH’ına ve ilaç-film arası etkileşimine bağlı olarak farklılıklar taşıması gerektiğini göstermiştir. LbL tekniğiyle fonksiyonel filmlerin kontrol edilebilen özelliklerde hazırlanması ve ilaç salım uygulamalarında kullanılması oldukça ümit verici sonuçlar vermiştir.

Keywords

İbuprofen adsorpsiyon/desorpsiyon, ince film, polielektrolit çok tabakalar, QCM-D, tabakalı kaplama

Supporting Institution

TÜBİTAK

Project Number

112M290

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