Effects of Pleurotus Eryngii (DC.) Quél. Mushroom Extracts on Cell Proliferation in Breast and Colon Cancer Cell Lines

Year 2024, , 659 - 668, 30.09.2024

Ferah Cömert Önder , Gülce Davutlar , Alper Önder , Hakan Allı , Mehmet Ay

https://doi.org/10.28979/jarnas.1499726

Abstract

This study was aimed to collect Pleurotus eryngii (DC.) Quél. edible/medicinal mushroom from Çanakkale/Kumkale and to evaluate the antiproliferative effects of alcoholic extracts on breast and colon cancer cell lines. For this purpose, the Soxhlet method prepared methanol and ethanol:H2O (70:30) extracts by gradually increasing solvent polarities. Then, breast (MDA-MB-231, BT-549, BT-20, MCF-7) and colon (HT-29) cancer cell lines were treated with the extracts in increasing concentrations (0.05-0.5 mg/mL) for 48 h. In conclusion, methanol and aqueous ethanol extracts exhibited significant antiproliferative effects in cancer cell lines, according to the MTT assay. The cell viability in the triple-negative breast cancer (TNBC) MDA-MB-231 cell line was highly decreased by methanol extract at a very low concentration (0.1 mg/mL). Furthermore, methanol extract at 0.3 mg/mL reduced the percentage of cell viability in the HT-29 cell line. Aqueous ethanol extract showed antiproliferative activity in breast and colon cancer cell lines at 0.25 mg/mL concentration for 48 h applications. In addition, some bioactive components such as 4-hydroxy flavone, chrysin, and tannic acid of aqueous ethanol extract have been determined by High-Performance Liquid Chromatography (HPLC) analysis. As a result, this study may increase interest in the studies on the anti-cancer activities and the mechanisms of P. eryngii mushroom.

Keywords

Pleurotus eryngii (DC.) Quél., mushroom, antiproliferation, natural products, cancer

Thanks

The cancer cell lines were provided from Erciyes University and Çanakkale Onsekiz Mart University. We thank Ahmet Davutlar for collecting mushroom in Kumkale village Çanakkale, and Çanakkale Onsekiz Mart University Experimental Research Application and Research Center (ÇOMÜDAM) for laboratory facilities, and Iğdır University Research Laboratory Practice and Research Center (ALUM) for HPLC analysis. A representative workflow was generated by the help of https://www.biorender.com/

References

• K. Kumar, R. Mehra, R. P. F. Guiné, M. J. Lima, N. Kumar, R. Kaushik, N. Ahmed, A. N. Yadav, H. Kumar, Edible mushrooms: A comprehensive review on bioactive compounds with health benefits and processing aspects, National Library of Medicine 10 (12) (2021) 2996.
• G. Anusiya, U. Gowthama Prabu, N.V. Yamini, N. Sivarajasekar, K. Rambabu, G. Bharath, F. Banat, A review of the therapeutic and biological effects of edible and wild mushrooms, Bioengineered 12 (2) (2021) 11239-11268.
• R. Garcia-Rubio, H. C. de Oliveira, J. Rivera, N Trevijano-Contador, The fungal cell wall: Candida, cryptococcus and aspergillus species, Frontiers in Microbiology 10 (2020) 2993.
• A. Assemie, G. Abaya, The effect of edible mushroom on health and their biochemistry, International Journal of Microbiology 2022 (2022) 8744788.
• P. A. Ayeka, Potential of mushroom compounds as immunomodulators in cancer immunotherapy: A review, Evidence Based Complementary and Alternative Medicine (2018) 7271509.
• R. L. Siegel, K. D. Miller, N. S. Wagle, A. Jemal, Cancer statistics, CA Cancer Journal for Clinicians 73 (1) (2023) 17-48.
• M. Borówka, S. Łącki-Zynzeling, M. Nicze, S. Kozak, J. Chudek, adverse renal effects of anti-cancer immunotherapy: A review, Cancers Basel 14 (17) (2022) 4086.
• H. S. Chen, Y. F. Tsai, S. Lin, C. C. Lin, K. H. Khoo, C. H. Lin, C. H. Wong, Studies on the immuno-modulating and anti-tumor activities of Ganoderma lucidum (Reishi) polysaccharides, Bioorganic Medicinal Chemistry 12 (21) (2004) 5595-5601.
• M. B. Gariboldi, E. Marras, N. Ferrario, V. Vivona, P. Prini, F. Vignati, G Perletti, Anti-cancer potential of edible/medicinal mushrooms in breast cancer, International Journal of Molecular Sciences 24 (12) (2023) 10120.
• S. K. Panda, G. Sahoo, S. S. Swain, W. Luyten, Anti-cancer activities of mushrooms: A neglected source for drug discovery, Pharmaceuticals Basel 15 (2) (2022) 176.
• S. P. Wasser, Medicinal mushrooms as a source of anti-tumor and immunomodulating polysaccharides, Applied Microbiology and Biotechnology 60 (3) (2002) 258–274.
• A. M. Mariga, F. Pei, W. J. Yang, L. Y. Zhao, Y. N. Shao, D. K. Mugambi, Q. H. Hu, Immunopotentiation of Pleurotus eryngii (DC. ex Fr.) quel, Journal of Ethnopharmacology 153 (3) (2014) 604-14.
• X. Shi, Y. Zhao, Y. Jiao, T. Shi, X. Yang, ROS-dependent mitochondria molecular mechanisms underlying anti-tumor activity of Pleurotus abalonus acidic polysaccharides in human breast cancer MCF-7 cells, PLoS One 8 (5) (2013).
• M. C. Radhakrishnan, N. Peter, Biological Properties of silver nanoparticles from β (1–3) glucan isolated from the edible mushroom Pleurotus florida, Letters Applied NanoBioScience 10 (2020) 2096–2106.
• J-T. Lin, C-W. Liu, Y-C. Chen, C-C. Hu, L-D. Juang, C-C. Shiesh, D-J. Yang, Chemical composition, antioxidant and anti-inflammatory properties for ethanolic extracts from Pleurotus eryngii fruiting bodies harvested at different time, LWT - Food Science and Technology 55 (2014) 374–382.
• C. Sánchez, Cultivation of Pleurotus ostreatus and other edible mushrooms, Applied Microbiology and Biotechnology 85 (2010) 1321–1337.
• R. C. G. Corrêa, T. Brugnari, A. Bracht, R. M. Peralta, I. C. F. R. Ferreira, Biotechnological, nutritional and therapeutic uses of Pleurotus spp. (Oyster mushroom) related with its chemical composition: A review on the past decade findings, Trends in Food Science and Technology 50 (2016) 103–117.
• D. Sande, G. P. de Oliveira, M. A. F. Moura, B. de Almeida Martins, M. T. N. S. Lima, J. A. Takahashi, Edible mushrooms as a ubiquitous source of essential fatty acids, Food Research International 125 (2018) 108524.
• H. Allı, S. Özderin, İ. Kıvrak, Ege bölgesinde yetişen körek mantarı (Pleurotus eryngii (DC.) quél.)’nın yağ asidi içeriğinin belirlenmesi, Mantar Dergisi 13 (3) (2022) 26-31.
• S. W. Kim, H. G. Kim, B. E. Lee, H. H. Hwang, D. H. Baek, S. Y. Ko, Effects of mushroom, Pleurotus eryngii, extracts on bone metabolism, Clinical Nutrition 25 (1) (2006) 166–170.
• M. Stajić, J. Vukojeviċ, V. Sonja, Biology of Pleurotus eryngii and role in biotechnological processes: A review, Critical Reviews in Biotechnology 29 (1) (2009) 55–66.
• Y. Kocak, I. Meydan, T. Gur Karahan, F. Sen, Investigation of mycosynthesized silver nanoparticles by the mushroom Pleurotus eryngii in biomedical applications, International Journal of Environmental Science and Technology 20 (2023) 4861–4872.
• F. Cömert Önder, S. Kalın, Ö. Maraba, A. Önder, P. Ilgın, E. Karabacak, Anti-cancer, antioxidant, antimicrobial activities, and HPLC analysis of alcoholic extracts of Parthenocissus quinquefolia l. plant collected from Çanakkale, Journal of Advanced Research in Natural and Applied Sciences 10 (1) (2024) 116-133.
• K. Sorimachi, K. Akimoto, Y. Ikehara, K. Inafuku, A. Okubo, S. Yamazaki, Secretion of TNF-α, IL-8 and nitric oxide by macrophages activated with Agaricus blazei Murill fractions in vitro, Cell Structure and Function 26 (2) (2001) 103–108.
• B. S. Min, J. J. Gao, N. Nakamura, M. Hattori, Triterpenes from the spores of Ganoderma lucidum and their cytotoxicity against Meth-A and LLC tumor cells, Pharmaceutical Bulletin 48 (7) (2000) 1026–1033.
• S. C. Hsu, C. C. Ou, J. W. Li, T.C. Chuang, H. P. Kuo, J. Y. Liu, C. S. Chen, S. C. Lin, C. H. Su, M. C. Kao, Ganoderma tsugae extracts inhibit colorectal cancer cell growth via G2/M cell cycle arrest, Journal of Ethnopharmacology 120 (3) (2008) 394–401.
• L. Li, Y. Wang, Progress on experimental research and clinical application of trametes robiniophila, China Cancer 16 (2006) 110–113.
• U. Munawer, V. B. Raghavendra, S. Ningaraju, K. L. Krishna, A. R. Ghosh, G. Melappa, A. Pugazhendhi, Biofabrication of gold nanoparticles mediated by the endophytic Cladosporium species: Photodegradation, in vitro anti-cancer activity and in vivo anti-tumor studies, International Journal of Pharmaceutics 588 (2020).
• M. van Barele, B. A. M. Heemskerk-Gerritsen, Y. V. Louwers, M. B. Vastbinder, J. W. M. Martens, M. J. Hooning, A. Jager, Estrogens and progestogens in triple negative breast cancer: do they harm?, Cancers Basel 13 (11) (2021) 2506.
• Q. Wu, S. Siddharth, D. Sharma, Triple negative breast cancer: A mountain yet to be scaled despite the triumphs, Cancers Basel 13 (15) (2021) 3697.
• F. T. Kolligs, Diagnostics and epidemiology of colorectal cancer, Visceral Medicine 32 (3) (2016) 158-64.
• M. P. Pathak, K. Pathak, R. Saikia, U. Gogoi, M. Z. Ahmad, P. Patowary, A. Das, Immunomodulatory effect of mushrooms and their bioactive compounds in cancer: A comprehensive review, Biomedicine and Pharmacotherapy 149 (2022) 112901.
• F. S. Reis, A. Martins, L. Barros, I. C. Ferreira, Antioxidant properties and phenolic profile of the most widely appreciated cultivated mushrooms: A comparative study between in vivo and in vitro samples, Food and Chemical Toxicology 50 (5) (2012) 1201-1227.
• D. Ren, N. Wang, J. Guo, L. Yuan, X. Yang, Chemical characterization of Pleurotus eryngii polysaccharide and its tumor-inhibitory effects against human hepatoblastoma HepG-2 cells, Carbohydrate Polymers 138 (2016) 123-133.