The Effect of Virgin Olive Oil Phenolics Against Oxidative Mitochondrial and Nuclear DNA in Human Peripheral Blood Mononuclear Cells

Year 2020, Volume: 6 Issue: 2, 231 - 238, 29.12.2020

Özlem Erol Tınaztepe

https://doi.org/10.28979/jarnas.844774

Cited By: 1

Abstract

In recent years, evidence has accumulated that the beneficial effects of olive oil are related to not only its high oleic acid content, but also the antioxidant activity of its phenolic components. This study examined the effect of virgin olive oil phenolic extract (VOPE) on basal and oxidative stress-induced DNA damage within nuclear and mitochon-drial genomes of human peripheral blood mononuclear cells (PBMCs). Cells were isolated from the blood samples of the healthy volunteers, 35 year old two male and two female. Cells were pre-treated with VOPE at a dose range that is dietary-relevant and non-cytotoxic. To create oxidative stress, cells were exposed to hydrogen peroxide (H2O2, 200 µM) for 30 minutes. The DNA damages were measured by gene-specific QPCR method. Pretreatment with VOPE did not change the level of basal DNA damage in both mitochondrial DNA (mtDNA) and nuclear DNA (nDNA). H2O2-induced oxidative stress caused two-fold higher DNA damage in mtDNA (2.76 lesions/10kb) than nDNA (1.43 lesions/10kb). However, VOPE treatment before oxidative stress did not show any protective effects on both mtDNA and nDNA. As a result, two different phenolic extract concentrations that could be found in the tissues and vascular system after virgin olive oil consumption do not have a capacity to prevent oxidative DNA damage in human PBMCs. These results indicate that the beneficial effect of olive oil, which is known to have more on the digestive system, may be due to its higher concentrations immediately after consumption.

Keywords

Phenolic compounds, , gene-specific QPCR, oxidative DNA damage, oxidative stress

Sızma Zeytinyağı Fenoliklerinin İnsan Periferik Kan Mononükleer Hücrelerinde Oksidatif Mitokondriyal ve Nükleer DNA Hasarına Karşı Etkisi

Abstract

Son yıllarda, zeytinyağının faydalı etkilerinin sadece yüksek oleik asit içeriğiyle ilişkili değil, aynı zamanda içerdiği fenolik bileşenlerin antioksidan aktivitesiyle de ilişkili olduğu kanıtlanmıştır. Bu çalışmada, sızma zeytinyağı fenolik ekstraktının (ZFE), insan periferik kan mononükleer hücrelerinin (PKMH) nükleer ve mitokondriyal DNA'sındaki bazal DNA hasarı ve oksidatif stres kaynaklı DNA hasarı üzerindeki etkisi incelendi. Hücreler, 35 yaşındaki sağlıklı iki erkek ve iki kadın gönüllünün kan örneklerinden izole edildi. Hücrelere diyetle alınabilecek ve sitotoksik etkisi olmayan ZFE konsantrasyonları ile ön-uygulama yapıldı. Hücreler üzerinde oksidatif stres oluş-turmak için 30 dakika hidrojen peroksit (H2O2, 200 µM) ile uygulama yapıldı. DNA hasarları gene-özgü QPCR yöntemi ile ölçüldü. Hücrelere ZFE ile ön uygulama yapılması her iki genomdaki bazal DNA hasar seviyesi üzerin-de olumsuz bir etkiye neden olmadı. H2O2 ile oluşturulan oksidatif stres, nDNA (nükleer DNA) (1.43 hasar/10kb) ile karşılaştırıldığında mtDNA'da (mitokondriyal DNA) (2.76 hasar/10kb) iki kat daha fazla hasara neden oldu. Bununla birlikte, oksidatif stres öncesi ZFE ile ön-uygulama yapılması hem mtDNA hem de nDNA üzerinde koru-yucu etki göstermedi. Sonuçta, sızma zeytinyağı tüketimi sonrası doku ve vasküler sistemde bulunabilecek iki farklı fenolik ekstrakt konsantrasyonunun, insan PKMH’lerde oksidatif DNA hasarlarını önleyici bir etkiye sahip olmadığı belirlendi. Bu sonuçlar, zeytinyağının sindirim sistemi üzerinde daha fazla sahip olduğu bilinen faydalı etkisinin tüketiminden hemen sonraki daha yüksek konsantrasyonlarından kaynaklanabileceğini işaret etmektedir

Keywords

Fenolik bileşikler, gene-özgü QPCR, oksidatif DNA hasarı, oksidatif stres, zeytinyağı

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