Effects of Pleurotus Eryngii (DC.) Quél. Mushroom Extracts on Cell Proliferation in Breast and Colon Cancer Cell Lines

Year 2024, Volume: 10 Issue: 3, 659 - 668, 30.09.2024

Ferah Cömert Önder , Gülce Davutlar , Alper Önder , Hakan Allı , Mehmet Ay

https://doi.org/10.28979/jarnas.1499726

Abstract

This study was aimed to collect Pleurotus eryngii (DC.) Quél. edible/medicinal mushroom from Çanakkale/Kumkale and to evaluate the antiproliferative effects of alcoholic extracts on breast and colon cancer cell lines. For this purpose, the Soxhlet method prepared methanol and ethanol:H2O (70:30) extracts by gradually increasing solvent polarities. Then, breast (MDA-MB-231, BT-549, BT-20, MCF-7) and colon (HT-29) cancer cell lines were treated with the extracts in increasing concentrations (0.05-0.5 mg/mL) for 48 h. In conclusion, methanol and aqueous ethanol extracts exhibited significant antiproliferative effects in cancer cell lines, according to the MTT assay. The cell viability in the triple-negative breast cancer (TNBC) MDA-MB-231 cell line was highly decreased by methanol extract at a very low concentration (0.1 mg/mL). Furthermore, methanol extract at 0.3 mg/mL reduced the percentage of cell viability in the HT-29 cell line. Aqueous ethanol extract showed antiproliferative activity in breast and colon cancer cell lines at 0.25 mg/mL concentration for 48 h applications. In addition, some bioactive components such as 4-hydroxy flavone, chrysin, and tannic acid of aqueous ethanol extract have been determined by High-Performance Liquid Chromatography (HPLC) analysis. As a result, this study may increase interest in the studies on the anti-cancer activities and the mechanisms of P. eryngii mushroom.

Keywords

Pleurotus eryngii (DC.) Quél., mushroom, antiproliferation, natural products, cancer

Thanks

The cancer cell lines were provided from Erciyes University and Çanakkale Onsekiz Mart University. We thank Ahmet Davutlar for collecting mushroom in Kumkale village Çanakkale, and Çanakkale Onsekiz Mart University Experimental Research Application and Research Center (ÇOMÜDAM) for laboratory facilities, and Iğdır University Research Laboratory Practice and Research Center (ALUM) for HPLC analysis. A representative workflow was generated by the help of https://www.biorender.com/

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