Mating Behavior and Hormone Profile in Melatonin Treatment for Goat Buck

Year 2021, Volume: 4 Issue: 2, 153 - 163, 27.12.2021

Cemil Tölü Nazif Yazgan Hande Işıl Akbağ

https://doi.org/10.51970/jasp.1019391

Abstract

In this study, the effects of melatonin implant treatment before the breeding season on melatonin (M) and testosterone (T) hormone levels, and the mating behavior and performance, were determined in Turkish Saanen goat bucks. Four head of bucks and 50 head of goats were used. The bucks were randomly divided into two groups and they were treated with 3 implants with 18 mg melatonin. The half of female goats were also implanted with melatonin (18 mg/goat). A total of 13 blood samples were collected before the melatonin implant (Day 0) and on the 21st, 42nd, 63rd, 94th, 129th, 157th, 185th, 213rd, 251st, 275th, 307th, and 335th days after the implant. Hormone analyses were performed using ELISA method and in animal species specific hormone kits. Mating was carried out for 10 minutes, and the mating behaviors of the bucks and does were recorded. While the treatment (MI) group had an overall means of melatonin hormone level of 526.3 ng/L, the control (C) group had a melatonin hormone level of 199.0 ng/L in overall (P=0.0224). The testosterone hormone level in the MI group was 12.7 nmol/L on average, and it was 6.13 nmol/L in the C group (P=0.2137). The MI group had significantly higher melatonin hormone levels in the summer, autumn, and spring than the C group, and significantly higher testosterone hormone levels in all seasons (P≤0.05). While M hormone had the highest level of MI and C groups in March, T hormone was highest of MI group in April and C group in March. Mating behaviors and the duration of mating were similar in the groups of bucks (P>0.05). A high statistically significant positive correlation coefficient (r=0.678) was determined between melatonin hormone and testosterone hormone (P<0.0001). In addition, there was a significant and positive correlation coefficient between hormone levels and the frequency of tongue-lapping (P=0.0134), sexual vocalization behavior (P=0.0199) in the MI group. In conclusion, the treatment of melatonin implants before the breeding season significantly increased the levels of M and T hormones and positive effects on frequency of mating behavior in Turkish Saanen goat bucks.

Keywords

Turkish Saanen, Implant, Melatonin, Testosterone, Sexual behavior

Supporting Institution

Çanakkale Onsekiz Mart Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

FHD-2019-2842

Thanks

This work was supported by Çanakkale Onsekiz Mart University, The Scientific Research Coordination Unit, Project number FHD-2019-2842.

Melatonin Uygulanan Tekelerde Aşım Davranışları ve Hormon Profili

Abstract

Bu çalışmada, aşım sezonu öncesinde melatonin uygulamasının Türk Saanen ırkı tekelerde melatonin (M) ve testosteron (T) hormon seviyeleri ile aşım davranışı ve performansına etkisi belirlenmiştir. Çalışmada dört baş teke ve 50 baş keçi kullanılmıştır. Çalışmada tekeler şansa bağlı olarak iki gruba ayrılarak 18 mg melatonin (M) içeren 3 adet implant yapılmıştır. Benzer şekilde keçilerin yarısına da melatonin implantı (18 mg/keçi) ugulanmıştır. Çalışmada melatonin implantı öncesi (0. Gün), implant sonrası 21., 42., 63., 94., 129., 157., 185., 213., 251., 275., 307. ve 335. Günler olmak üzere toplam 13 defa kan örneği alınmıştır. Hormon analizleri ELISA yöntemi kullanılarak ve hayvan türüne özgü hormon kitleriyle yapılmıştır. Çiftleştirmeler 10 dk. süreyle yapılmış, erkek ve dişilerde eşeysel davranışlar gözlenmiştir. Tekelerde muamele (MI) grubu genel ortalamada 526.3 ng/L M hormon seviyesine sahip olurken, kontrol (C) grubu 199.0 ng/L M hormon seviyesine sahip olmuştur (P=0.0224). MI grubunda T hormon seviyesi ortalama 12.7 nmol/L olurken, C grubunda ise 6.13 nmol/L olmuştur (P=0.2137). Tekelerde yaz, sonbahar ve ilkbahar mevsimlerinde M hormonu seviyeleri, T hormonunda ise tüm mevsimlerde MI grubu C grubundan önemli ölçüde daha yüksek hormon seviyelerine sahip olmuştur (P≤0.05). MI ve C gruplarında mart ayında yüksek düzeyde M hormonu olurken, T hormonu MI grubunda en yüksek nisan ayında, C grubunda mart ayında belirlenmiştir. Tekelerde aşım sırasındaki eşeysel davranışlar ile aşım süreleri gruplara göre benzer gerçekleşmiştir (P>0.05). Çalışmada, melatonin hormonu ile testosteron hormonu arasında pozitif yönde yüksek ve istatistiksel olarak önemli korelasyon katsayısı (r=0.678) belirlenmiştir (P<0.0001). Ayrıca MI grubunda hormon seviyeleri ile dil çıkarma sıklığı (P=0.0134) ve seksüel ses sıklığı (P=0.0134) arasında önemli önemli düzeyde ve pozitif yönde korelasyonlar belirlenmiştir. Sonuçta, Türk Saanen keçisi tekelerinde üreme mevsiminden önce melatonin implantı M ve T hormon düzeylerini önemli ölçüde artırmış ve aşım davranışı sıklığı üzerinde olumlu etkiler yapmıştır.

Keywords

Türk Saanen, İmplant, Melatonin, Testosteron, Seksüel davranış

Project Number

FHD-2019-2842

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