DOES THE DISINFECTANT EFFICACY OF THE 2% GLUTERALDEHIDE SOLUTION CHANGE WITH THE AGING OF THE SOLUTION?

Abstract

Purpose:In our study, it was aimed to determine whether the disinfectant efficacy of 2% glutaraldehyde solution changes with the aging of the solution. Methods: In our study, metal obtained from k-wire, plastic obtained from serum sets, and glass balls were used to imitate metal, plastic, and glass materials found in surgical instruments. 20 of each material were contaminated with Klebsiella pneumoniae (K. pneumoniae), Staphylococcus aureus (S. aureus),Pseudomonas aeruginosa (P. aeruginosa), Escherichia coli (E. coli), and Yeast on the 1st, 14th, and 28th days of the solution. 10 of them were thrown into 2% glutaraldehyde solution in different glasses and kept in the solution for 15 minutes. Subsequently, materials were taken from the glasses using separate sterile forceps for each piece. The other 10 were washed with physiological saline solution for 1 minute. After the procedures, they were sent to the microbiology laboratory without waiting and inoculated on routine media, and Gram staining was performed simultaneously. The identification of strains was carried out by conventional methods. Results: No growth was detected on the 1st, 14th, and 28th days in all metal, plastic, and glass materials disinfected with 2% alkaline glutaraldehyde solution. In the control groups, growth rates were 53.3%, 50%, and 63.3%, respectively. Conclusions: If the guidelines and manufacturer's recommendations are followed in the preparation and use of 2% alkaline glutaraldehyde solution, it provides an effective disinfection without being affected by the aging of the solution.

Keywords

• İnfection
• Sterilization
• Disinfection
• Gluteraldehyde
• Arthroscopic instrument

%2 GLUTERALDEHİT SOLUSYONUNUN DEZENFEKTAN ETKİNLİĞİ SOLUSYONUN YAŞLANMASI İLE DEĞİŞİR Mİ?

Abstract

Giriş:Çalışmamızda %2 gluteraldehit solusyonunun dezenfektan etkinliğinin solusyonun yaşlanması ile değişime uğrayıp uğramadığının tespiti amaçlanmıştır. Materyal-metot:Çalışmamızda cerrahi aletlerde bulunan metal, plastik ve cam malzemeleri taklit etmek adına 2 cm uzunluğunda k telinden elde edilen metal, serum setlerinden elde edilen plastik ve cam bilyeler kullanıldı. Her birinden 20’şer adeti solusyonun1.,14. ve 28. gününde Klepsiella Pneumoniae, Staphilococcus Aerius, Pseudomonas Aeruginosa, Escherichia Coli ve Maya ile kontamine edildi. 10’ar tanesi %2 gluteraldehit solusyon havuzundan plastik pet bardaklara alınan gluteraldehit solusyonu içine her bir parça ayrı pet bardaklarda olacak şekilde atıldı ve 15 dakika süre ile solusyon içerisinde bekletildi. Sonrasında malzemeler bardaklardan her bir parça için ayrı steril forseps kullanılarak alındı. Diğer 10’ar adeti 1 dakika boyunca serum fizyolojik solüsyon ile yıkandı. İşlemler sonrasında bekletilmeden mikrobiyoloji laboratuarına gönderilerek EMB agar ve kanlı agarda kültürlendi. Sonuçlar: %2 alkalen gluteral solüsyonu ile dezenfekte edilen metal, plastik ve cam malzemelerde 1.,14. ve 28. günde üreme tespit edilemedi. Kontrol gruplarında ise sırası ile %53.3, %50 ve %63.3 oranlarında kontaminasyonda kullanılan mikroorganizmalardan bir ve ya birkaçında üreme tespit edildi. Çıkarımlar:%2 alkalen gluteral solüsyonu hazırlanması ve kullanılmasında kılavuz ve üretici önerilerine dikkat edilmesi halinde solüsyonun yaşlanmasından etkilenmeden aktif olduğu süre boyunca etkin bir dezenfeksiyon sağlar.

Keywords

• İnfection
• Sterilization
• Disinfection
• Gluteraldehyde
• Arthroscopic instrument

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