Dietilnitrozamin uygulanan sıçanlarda düşük doz Epigallocatechin Gallate'nin biyokimyasal, hematolojik, periferik yayma ve ağırlık değerlendirilmesi

Abstract

Arka plan: Günümüzde teknolojinin gelişmesiyle birlikte başta Dietilnitrozamin (DEN) olmak üzere birçok kimyasalın tarım ve sanayide kullanımı artmıştır. Epigallocatechin gallate'nin (EGCG) polifenolik bileşikleri, yeşil çayın aktif bileşenidir. Yeşil çayın antioksidan etkileri olduğu bildirilmiştir. Bu çalışmada, düşük doz Epigallocatechin gallate (EGCG)'nin Dietilnitrozamin uygulanan sıçanlara maruz kalmaya karşı etkileri incelenmiştir. Yöntemler: Gruplar uygulama için Kontrol, Sham, DEN EGCG ve DEN+EGCG olmak üzere on rattan oluşan beş gruba ayrıldı. Analiz edilen parametreler hemogram, biyokimyasal, periferik yayma ve ağırlıktır. Bulgular: DEN enjeksiyonu, hepatosit yaralanmalarının belirtileri olan LDH, AST, ALT ve ALP değerlerini önemli ölçüde artırdı. EGCG grubunda WBC sayısı arttı. HDL ve TChol düzeyleri açısından DEN+EGCG'nin birlikte uygulandığı grup en yüksek, TG ve LDL düzeyleri ise en düşük bulundu. Mevcut çalışma, düşük doz EGCG'nin DEN uygulanan sıçanlara karşı etkilerini gösteren kapsamlı bir çalışma olacaktır.

Keywords

• Biochemical
• Diethylnitrosamine
• EGCG
• hematological
• peripheral smear
• rat

Biochemical, hematological, peripheral smear and weight evaluation of low dose Epigallocatechin Gallate in Diethylnitrosamine administered rats

Abstract

Background: Currently, with the development of technology, the use of many chemicals especially Diethylnitrosamine (DEN) in agriculture and industry has increased. The polyphenolic compounds of Epigallocatechin gallate (EGCG) is the active ingredient of green tea. It has been reported that green tea has antioxidant effects. In this study, effects of low dose Epigallocatechin gallate (EGCG), against exposure of Diethylnitrosamine administered rats. Methods: As a group, groups were divided into five groups of ten rats for the application as Control, Sham, DEN EGCG and DEN+EGCG. The parameters analyzed are hemogram, biochemical, peripheral smear and weight. Results: DEN injection has significantly increased LDH, AST, ALT and ALP values, Which are signs of hepatocyte injuries. The number of WBCs increased in the EGCG group. In terms of HDL and TChol levels, the group in which DEN+EGCG were applied together was found to be the highest and TG and LDL levels were found to be lowest. The current study will be a comprehensive study demonstrating the effects of low-dose EGCG against DEN-administred rats.

Keywords

• Biochemical
• Diethylnitrosamine
• EGCG
• hematological
• peripheral smear
• rat

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