The Effect of Artemisinin on Oxidative Stress Markers in Mouse Heart and Lung Tissues in an Experimental Model of Epileptic Seizure.

Abstract

Aim: The current study investigated the effects of artemisinin on the heart and lung tissue against pentylenetetrazol-induced seizures in mice. For this purpose, malondialdehyde (MDA), advanced oxidation protein products (AOPP), Catalase (CAT), glutathione (GSH), and glutathione peroxidase (GSH-Px) levels were evaluated in both tissue homogenates.

Material and Method: Swiss albino male mice (n=42) were used in the experiment. Animals were divided into six groups; Control (C), pentylenetetrazol (PTZ), valproate 100 mg/kg (VPA), artemisinin 30 mg/kg (ARS)+PTZ, ARS 60 mg/kg+PTZ, ARS 120 mg/kg+PTZ. On the 26th day of the experiment, the mice were sacrificed and the samples were kept at -80 0C for biochemical analysis.

Results: There were significant differences in the five biochemical parameters analyzed in heart and lung tissues. Heart and lung MDA levels of the PTZ group were found to be significantly higher than the C and ARS-60 groups (p<0.05). Heart and lung MDA levels of the PTZ group were found to be significantly higher than the C and ARS-60 groups. Likewise, heart AOPP levels decreased significantly in the VPA and ARS-60 groups compared to the PTZ group (p<0.05). There was no significant difference between the groups in terms of lung AOPP levels (p>0.05). Heart CAT and GSH levels were decreased in the PTZ group compared to the other groups. However, in terms of Lung CAT levels, the PTZ group had the highest value compared to the other groups, while it had the lowest value in terms of GSH level. The GSH-Px level did not differ significantly between the groups in heart tissue (p>0.05). The lung GSH-Px level was significantly increased in the ARS-30 group when compared to the PTZ group (p<0.05).

Conclusion: Consequently ARS treatment can inhibit PTZ-induced oxidative stress in peripheral tissues. In addition, ARS may provide improvements in decreased antioxidant enzymes. ARS may contribute to the antioxidant defense system.

Keywords

• Artemisinin
• Pentylenetetrazol
• oxidative stress
• Antioxidant
• Heart
• Lung

Deneysel Epileptik Nöbet Modelinde, Artemisinin'in Fare Kalp ve Akciğer Dokularında Oksidatif Stress Belirteçleri Üzerine Etkisi.

Abstract

Amaç: Çalışmamızın amacı, kalp ve akçiğer doku homojenatlarında malondialdehit (MDA) ve advanced oxidation protein products (AOPP), catalase (CAT), glutathione (GSH) ve glutathione peroxidase (GSH-Px) düzeylerinin farelerde deneysel PTZ-kindling modelinde oluşan nöbetlere karşı etkisi araştırılmıştır. Gereç ve Yöntem: Deneyde Swiss albino erkek fareler (n=42) kullanıldı. Hayvanlar Kontrol, pentilenetetrazol 35 mg/kg (PTZ) , valproat 100 mg/kg (VPA), artemisinin 30 mg/kg (ARS),+ PTZ, ARS 60 mg/kg+PTZ, ARS 120 mg/kg+PTZ olmak üzere altı gruba ayrıldı. Deneyin 26. gününde farelrer sakrifiye edilerek, Biyokimyasal analizler için numuneler -80 0C'de muhafaza edildi. Bulgular: Kalp ve akciğer dokularında analiz edilen beş biyokimyasal parametrede anlamlı farklar vardı. PTZ grubunun, Kalp ve akciğer MDA düzeyleri C ve ARS-60 grubuna göre anlamlı olarak yüksek bulundu ( p < 0.05). PTZ grubunun, Kalp ve akciğer MDA düzeyleri C ve ARS-60 grubuna göre anlamlı olarak yüksek bulundu. Aynı şekilde kalp'te AOPP düzeyleri VPA ve ARS-60 gruplarında anlamlı bir azalış sergilerken ( p < 0.05), akçiğerde ise gruplar arasında anlamlı bir fark yoktu ( p > 0.05). PTZ grubunun kalp'te CAT ve GSH düzeyleri diğer gruplara göre azalırken, akciğerde CAT düzeyi artığı, GSH düzeyininde azaldığı bulundu. kalp dokusundaki GSH-Px düzeyinde gruplar arasında anlamlı bir fark bulunmadı (p >0.05). GSH-Px düzeyi, akciğerde sadece ARS-30 grubunda analmlı artış göstermiştir (p < 0.05). Sonuç: Sonuç olarak, PTZ işlem görmüş farelerde oluşan nöbertlerin periferal dokularda oksidatif strese neden olur. ARS tedavisi antioksidan savunma sistemine katkı sağlayabilir.

Keywords

• Artemisinin
• Pentilentetrazol
• Oksidatif stres
• Antioksidan
• Kalp
• Akciğer

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