The Effect of Exercise on Serum Resistin and Leptin Values in Rats Fed with a High Fat Diet

Abstract

Aim: This study was performed to investigate the effect of exercise on serum resistin and leptin values in rats fed with a high-fat diet. Material and Method: 24 Wistar albino male rats were used in the study. They were randomly divided into 4 groups, with 6 rats in each group. The groups were determined as the control group (C), exercise group (E), high-fat diet (HFD) group, and high-fat diet + exercise (HFDE) Group. Results: When the findings obtained in this study were evaluated statistically, it was determined that the resistin values were similar in the C, E and HFDE groups, and higher in the HFD group compared to the other groups. It was observed that resistin value increased with high-fat diet and decreased with exercise. When evaluated in terms of leptin levels, the C and E groups showed similarity, while the HFD and HFDE groups showed similarity with each other. Although exercise decreased the leptin level, which was highly increased with a high-fat diet, it was not statistically significant. Conclusion: It has been determined that feeding with a high-fat diet causes an increase in serum resistin and leptin levels, and exercise provides a significant decrease in resistin values, but is not effective in leptin levels.

Keywords

• Resistin
• Leptin
• Exercise
• High-fat diet

Project Number

Project No: 2015 –VSYO-B256

Yüksek Yağlı Diyetle Beslenen Ratlarda Egzersizin Serum Resistin ve Leptin Değerlerine Etkisi

Abstract

Amaç: Bu çalışma, yüksek yağlı diyetle beslenen ratlarda egzersizin serum resistin ve leptin değerlerine nasıl bir etkisinin olacağını araştırmak için yapıldı. Gereç ve Yöntem: Çalışmada 24 adet Wistar albino ırkı erkek rat kullanıldı. Her grupta 6 adet rat olacak şekilde rastgele 4 gruba ayrıldı. Gruplar kontrol grubu (K), egzersiz grubu (E), yüksek yağlı diyet (YYD) grubu ve yüksek yağlı diyet + egzersiz (YYDE) Grubu olarak belirlendi. Bulgular: Yapılan bu araştırmada elde edilen bulgular istatiksel olarak değerlendirildiğinde K, E ve YYDE gruplarında resistin değerlerinin benzer olduğu, YYD grubunda ise diğer gruplara göre yüksek olduğu tespit edilmiştir. Yüksek yağlı diyet ile artan resistin değerinin egzersiz ile düştüğü görülmüştür. Leptin seviyeleri açısından değerlendirildiğinde K ve E grubu benzerlik gösterirken, YYD ve YYDE grupları da birbiri ile benzerlik göstermektedir. Yüksek yağlı diyet ile oldukça artan leptin seviyesini egzersiz bir miktar düşürse de, istatistiksel açıdan anlamlı bulunmamıştır. Sonuç: Yüksek yağlı diyet ile beslenmenin serum resistin ve leptin düzeylerinde artışa sebep olduğu, egzersizin resistin değerlerinde anlamlı bir düşüş sağladığı fakat leptin düzeylerinde etkili olmadığı tespit edilmiştir.

Keywords

• Resistin
• Leptin
• Exercise
• High-fat diet

Supporting Institution

Van Yüzüncü Yıl Üniversitesi

Project Number

Project No: 2015 –VSYO-B256

References

1. 1. Mosby TT, Cosgrove M, Sarkardei S, et al. Nutrition in adult and childhood cancer: role of carcinogens and anti-carcinogens. Anticancer research 2012; 32(10): 4171-4192.
2. 2. Toumilehto J, Lindström J, Eriksson JG, et al. Prevention of type 2 diabetes mellitus by changes in lifestyle among subjects with impaired glucose tolerance. N Engl J Med 2001; 344: 1343-1350.
3. 3. Swinburn BA, Caterson I, Seidell JC, et al. Diet, nutrition and the prevention of excess weight gain and obesity. Public health nutrition 2004; 7(1a): 123-146.
4. 4. Kodama S, Tanaka S, Heianza Y, et al. Association between physical activity and risk of all-cause mortality and cardiovascular disease in patients with diabetes: a meta-analysis. Diabetes care 2013; 36(2): 471-479.
5. 5. Steppan CM, Bailey ST, Bhat S, et al. The hormone resistin links obesity to diabetes. Nature 2001; 409(6818): 307-312.
6. 6. Ukkola O. Resistin-a mediator of obesity-associated insulin resistance or an innocent bystander?. European journal of endocrinology 2002;147(5): 571-574.
7. 7. Lee GH, Proenca R, Montez JM, et al. Abnormal splicing of the leptin receptor in diabetic mice. Nature 1996; 379(6566):632-635.
8. 8. Fantuzzi G, Faggioni R. Leptin in the regulation of immunity, inflammation, and hematopoiesis. Journal of leukocyte biology 2000;68(4): 437-446.

9. 9. Keleş V, Büyükgüzel K, Büyükgüzel E. Leptin ve Metabolik Düzenlenmedeki Rolü. Türkiye Diyabet ve Obezite Dergisi, 2018; 2(1): 17-22.
10. 10. Yaşar S, Günbatar N, Yüksek S, et al. Effect of exercise on erythrocyte osmotic fragility in high fat diet fed rats during intermittent fasting. Eastern Journal Of Medicine, 2017; 22(4): 176.
11. 11. Günbatar, N, Bayıroğlu, F. The Effect of a Highly Saturated Fat Diet and Intermittent Fasting Diet on Experimental Colon Cancer Development and Some Serum Inflammation Markers in Rats, 1 Adiponectin and Lipid Metabolism . Van Veterinary Journal , 2015; 26 (3): 123-127 .
12. 12. Pagano C, Marin O, Calcagno A, et al. Increased serum resistin in adults with prader-willi syndrome is related to obesity and not to insulin resistance. The Journal of Clinical Endocrinology & Metabolism 2005; 90(7): 4335-4340.
13. 13. Steppan CM, Lazar MA. Resistin and obesity-associated insulin resistance. Trends in endocrinology & Metabolism 2002; 13(1): 18-23.
14. 14. Eminler AT, Aygun C, Konduk T, et al. The relationship between resistin and ghrelin levels with fibrosis in nonalcoholic fatty liver disease. Journal of research in medical sciences: the official journal of Isfahan University of Medical Sciences 2014;19 (11): 1058.
15. 15. Koerner A, Kratzsch J, Kiess W. Adipocytokines: leptin the classical, resistin the controversical, adiponectin—the promising, and more to come. Best practice & research Clinical endocrinology & metabolism 2005; 19(4): 525-546.
16. 16. Jung SH, Park HS, Kim KS, et al. Effect of weight loss on some serum cytokines in human obesity: increase in IL-10 after weight loss. The Journal of nutritional biochemistry 2008; 19(6): 371-375.
17. 17. Kadoglou NP, Perrea D, Iliadis F, et al. Exercise reduces resistin and inflammatory cytokines in patients with type 2 diabetes. Diabetes care 2007; 30(3): 719-721.
18. 18. Rajala MW, Obici S, Scherer PE, et al. Adipose-derived resistin and gut-derived resistin-like molecule–β selectively impair insulin action on glucose production. The Journal of clinical investigation 2003; 111(2): 225-230.
19. 19. Muse ED, Obici S, Bhanot S, et al. Role of resistin in diet-induced hepatic insulin resistance. The Journal of clinical investigation 2004; 114(2): 232-239.
20. 20. Palanivel R, Sweeney G. Regulation of fatty acid uptake and metabolism in L6 skeletal muscle cells by resistin. FEBS letters 2005;579(22): 5049-5054.
21. 21. Kraemer RR, Castracane VD. Exercise and humoral mediators of peripheral energy balance: ghrelin and adiponectin. Experimental biology and medicine 2007; 232(2): 184-194.
22. 22. Lin TC, Huang KW, Liu CW, et al. Leptin signaling axis specifically associates with clinical prognosis and is multifunctional in regulating cancer progression. Oncotarget 2018; 9(24): 17210.
23. 23. Correia ML, Haynes WG. Obesity-related hypertension: is there a role for selective leptin resistance?. Current hypertension reports 2004; 6(3): 230-235.
24. 24. Pagano C, Marzolo M, Granzotto M, et al. Acute Effects of Exercise on Circulating Leptin in Lean and Genetically Obesefa/faRats. Biochemical and biophysical research communications 1999; 255(3): 698-702.
25. 25. Landt M, Lawson GM, Helgeson JM, et al. Prolonged exercise decreases serum leptin concentrations. Metabolism 1997; 46(10): 1109-1112.
26. 26. Essig DA, Alderson NL, Ferguson MA, et al. Delayed effects of exercise on the plasma leptin concentration. Metabolism 2000;49(3): 395-399.
27. 27. Weltman A, Pritzlaff CJ, Wideman L, et al. Intensity of acute exercise does not affect serum leptin concentrations in young men. Medicine and science in sports and exercise 2000; 32(9): 1556-1561.
28. 28. Kraemer R., Acevedo E, Synovitz L, et al. Leptin and steroid hormone responses to exercise in adolescent female runners over a 7-week season. European journal of applied physiology 2001; 86(1): 85-91.
29. 29. Olive JL, Miller GD. Differential effects of maximal-and moderate-intensity runs on plasma leptin in healthy trained subjects. Nutrition 2001; 17(5): 365-369.
30. 30. Gökbel H., et al. "Ratlarda zorlu egzersizde serum leptin düzeylerindeki değişme ve çinko eksikliği/takviyesiyle ilişkisi. I." Leptin Sempozyumu 2003; 20-22.