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New proteins in the differentiation of papillary renal cell carcinoma from clear cell renal cell carcinomas; Importance of DARS2, Reelin and Enkurin

Year 2023, , 326 - 330, 22.03.2023
https://doi.org/10.16899/jcm.1255264

Abstract

Purpose: The purpose of the present study was to examine the roles of new proteins DARS2, Reelin, and Encurin in the differentiation of Clear-Cell Renal Cell Carcinoma (RCC) and Papillary Renal Cell Carcinoma (RCC). Clear-cell RCC is the most common malignancy of the kidney, and papillary RCC is the second most common malignant malignancy in this respect. They are neoplasms and show similarity to each other, both histologically and morphologically, in some cases. Differential diagnosis is important because treatment approaches and prognoses are different. Although careful histopathological examination and specific immunohistochemical markers are important for diagnosis, there are no specific antibodies that can be used reliably and the search for biomarkers continues in this regard.
Materials and Methods: A total of 30 Clear-Cell RCC and 30 Papillary RCC cases were included in the present study. Patients were identified retrospectively by reviewing the hospital database and pathological reports. Pathological data were obtained from hospital medical archives and pathology reports.
Results: It was found that DARS2, Reelin, and Encurin proteins were significantly higher in papillary RCC when compared to clear-cell RCC.
Conclusion: It was concluded that DARS2, Reelin, and Encurin proteins may be potential biomarkers for the differentiation of Papillary RCC and Clear-Cell RCC.

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References

  • 1. Chow WH, Dong LM, Devesa SS. Epidemiology and risk factors for kidney cancer. Nat Rev. Urol 2010; 7:245-257.
  • 2. D'Avella C, Abbosh P, Pal SK, Geynisman DM. Mutations in renal cell carcinoma. Urol Oncol 2020;38(10):763-773.
  • 3.Tretiakova MS, Sahoo S, Takahashi M, Turkyilmaz M, Vogelzang NJ, Lin F, et al. Expression of alphamethylacyl-CoA racemase in papillary renal cell carcinoma. Am J Surg Pathol 2004; 28: 69-76.
  • 4. Leroy X, Zini L, Leteure E, Zerimech F, Porchet N, Aubert JP, et al. Morphologic subtyping of papillary renal cell carcinoma: correlation with prognosis and differential expression of MUC1 between the two subtypes. Mod Pathol 2002; 15: 1126-30.
  • 5. Zong WX, Rabinowitz JD, White E. Mitochondria and cancer. Mole Cell 2016; 61: 667–676.
  • 6. Ucer O, Kocaman N. New candidates in the differential diagnosis of malignant mesothelioma from benign mesothelial hyperplasia and adenocarcinoma; DARS2 and suprabasin. Tissue and Cell 2022;79:101920.
  • 7. Ndoye A, Miskin RP, DiPersio CM. Integrin α3β1 Represses Reelin Expression in Breast Cancer Cells to Promote Invasion. Cancers (Basel) 2021;19:344.
  • 8.Hou R, Li Y, Luo X, et al. ENKUR expression induced by chemically synthesized cinobufotalin suppresses malignant activities of hepatocellular carcinoma by modulating β-catenin/c-Jun/MYH9/USP7/c-Myc axis. Int J Biol Sci 2022;18:2553-2567.
  • 9. Kocaman N, Artaş G. Can novel adipokines, asprosin and meteorin-like, be biomarkers for malignant mesothelioma? Biotech Histochem 2020;95(3):171-175.
  • 10. Dogan SA, Pujol C, Maiti P, et al. Tissue-specific loss of DARS2 activates stress responses independently of respiratory chain deficiency in the heart. Cell Metab 2014;19:458-469.
  • 11. He K, Guo X, Liu Y, Li J. TUFM downregulation induces epithelial-mesenchymal transition and invasion in lung cancer cells through a mechanism involving AMPK-GSK 3β signaling Cell Mol. Life Sci 2016;73:2105 – 2121.
  • 12. Su CY, Chang YC, Yang CJ, et al. The contrasting prognostic effect of NDUFS1 and NDUFS8 in lung cancer reflects the oncojanus role of the mitochondrial complex I. Sci Rep 2016; 6: 31357.
  • 13. Ishii K, Kubo I, Nakajima K. Reelin and Neuropsychiatric Disorders. Front Cell Neurosci 2016; 10:229.
  • 14.Serrano-Morales JM, Vázquez-Carretero MD, García-Miranda P, Carvajal AE, Calonge ML, Ilundain AA, et al. Reelin Protects against Colon Pathology via p53 and May Be a Biomarker for Colon Cancer Progression. Biology (Basel) 2022; 26;11:1406.
  • 15. Ndoye A, Miskin RP, DiPersio CM. Integrin α3β1 Represses Reelin Expression in Breast Cancer Cells to Promote Invasion. Cancers (Basel) 2021;13(2):344.
  • 16. Hou R, Li Y, Luo X, et al. ENKUR expression induced by chemically synthesized cinobufotalin suppresses malignant activities of hepatocellular carcinoma by modulating β-catenin/c-Jun/MYH9/USP7/c-Myc axis. Int J Biol Sci 2022;18(6):2553-2567.
  • 17. Song T, Zhou P, Sun C, He N, Li H, Ran J, et al. Enkurin domain containing 1 (ENKD1) regulates the proliferation, migration and invasion of non-small cell lung cancer cells. Asia Pac J Clin Oncol. 2022;18(2):39–45.
  • 18. Ma Q, Lu Y, Lin J, Gu Y. ENKUR acts as a tumor suppressor in lung adenocarcinoma cells through PI3K/Akt and MAPK/ERK signaling pathways. J Cancer 2019;10(17):3975–3984.
  • 19. Jain K, Basu A. Multifunctional protein kinase C-ε in cancer development and progression. Cancers 2014 6:860 – 878.

Papiller renal hücreli karsinomun berrak hücreli renal hücreli karsinomlardan ayrımında yeni proteinler; DARS2, Reelin ve Enkurin'in Önemi

Year 2023, , 326 - 330, 22.03.2023
https://doi.org/10.16899/jcm.1255264

Abstract

Amaç: Bu çalışma ile berrak hücreli böbrek hücreli karsinomlardan (BHK), papiller böbrek hücreli karsinom (BHK)’un ayırmında yeni proteinler olan DARS2, reelin ve enkurinin rollerinin incelenmesi amaçlandı. Berrak hücreli BHK, böbreğin en yaygın, papiller BHK ise ikinci sıklıkta görülen malign neoplazmları olup bazı durumlarda histolojik ve morfolojik olarak birbirleri ile benzerlik göstermektedir. Tedavi yaklaşımları ve prognozları farklı olduğu için ayırıcı tanıları önemlidir. Dikkatli histopatolojik inceleme ve spesifik immünohistokimyasal belirteçler tanı için önemli olmasına rağmen güvenilir bir şekilde kullanılabilecek spesifik antikorlar yoktur ve biyobelirteç arayışları devam etmektedir.
Gereç ve Yöntem: Bu çalışmaya 30 adet berrak hücreli BHK ve 30 adet de papiller BHK olgusu dahil edildi. Hastalar, patolojik bir veri tabanının gözden geçirilmesiyle geriye dönük olarak tanımlandı. Patolojik veriler hastane tıbbi arşivlerinden ve patoloji raporlarından elde edildi.
Bulgular: DARS2, reelin ve enkurin proteinleri papiller BHK da berrak hücreli BHK ya kıyasla anlamlı derecede yüksek bulundu.
Sonuç: DARS2, reelin ve enkurin proteinlerinin papiller BHK ve berrak hücreli BHK ayrımı için potansiyel birer biyobelirleyiciler olabileceği sonucuna varılmıştır.

References

  • 1. Chow WH, Dong LM, Devesa SS. Epidemiology and risk factors for kidney cancer. Nat Rev. Urol 2010; 7:245-257.
  • 2. D'Avella C, Abbosh P, Pal SK, Geynisman DM. Mutations in renal cell carcinoma. Urol Oncol 2020;38(10):763-773.
  • 3.Tretiakova MS, Sahoo S, Takahashi M, Turkyilmaz M, Vogelzang NJ, Lin F, et al. Expression of alphamethylacyl-CoA racemase in papillary renal cell carcinoma. Am J Surg Pathol 2004; 28: 69-76.
  • 4. Leroy X, Zini L, Leteure E, Zerimech F, Porchet N, Aubert JP, et al. Morphologic subtyping of papillary renal cell carcinoma: correlation with prognosis and differential expression of MUC1 between the two subtypes. Mod Pathol 2002; 15: 1126-30.
  • 5. Zong WX, Rabinowitz JD, White E. Mitochondria and cancer. Mole Cell 2016; 61: 667–676.
  • 6. Ucer O, Kocaman N. New candidates in the differential diagnosis of malignant mesothelioma from benign mesothelial hyperplasia and adenocarcinoma; DARS2 and suprabasin. Tissue and Cell 2022;79:101920.
  • 7. Ndoye A, Miskin RP, DiPersio CM. Integrin α3β1 Represses Reelin Expression in Breast Cancer Cells to Promote Invasion. Cancers (Basel) 2021;19:344.
  • 8.Hou R, Li Y, Luo X, et al. ENKUR expression induced by chemically synthesized cinobufotalin suppresses malignant activities of hepatocellular carcinoma by modulating β-catenin/c-Jun/MYH9/USP7/c-Myc axis. Int J Biol Sci 2022;18:2553-2567.
  • 9. Kocaman N, Artaş G. Can novel adipokines, asprosin and meteorin-like, be biomarkers for malignant mesothelioma? Biotech Histochem 2020;95(3):171-175.
  • 10. Dogan SA, Pujol C, Maiti P, et al. Tissue-specific loss of DARS2 activates stress responses independently of respiratory chain deficiency in the heart. Cell Metab 2014;19:458-469.
  • 11. He K, Guo X, Liu Y, Li J. TUFM downregulation induces epithelial-mesenchymal transition and invasion in lung cancer cells through a mechanism involving AMPK-GSK 3β signaling Cell Mol. Life Sci 2016;73:2105 – 2121.
  • 12. Su CY, Chang YC, Yang CJ, et al. The contrasting prognostic effect of NDUFS1 and NDUFS8 in lung cancer reflects the oncojanus role of the mitochondrial complex I. Sci Rep 2016; 6: 31357.
  • 13. Ishii K, Kubo I, Nakajima K. Reelin and Neuropsychiatric Disorders. Front Cell Neurosci 2016; 10:229.
  • 14.Serrano-Morales JM, Vázquez-Carretero MD, García-Miranda P, Carvajal AE, Calonge ML, Ilundain AA, et al. Reelin Protects against Colon Pathology via p53 and May Be a Biomarker for Colon Cancer Progression. Biology (Basel) 2022; 26;11:1406.
  • 15. Ndoye A, Miskin RP, DiPersio CM. Integrin α3β1 Represses Reelin Expression in Breast Cancer Cells to Promote Invasion. Cancers (Basel) 2021;13(2):344.
  • 16. Hou R, Li Y, Luo X, et al. ENKUR expression induced by chemically synthesized cinobufotalin suppresses malignant activities of hepatocellular carcinoma by modulating β-catenin/c-Jun/MYH9/USP7/c-Myc axis. Int J Biol Sci 2022;18(6):2553-2567.
  • 17. Song T, Zhou P, Sun C, He N, Li H, Ran J, et al. Enkurin domain containing 1 (ENKD1) regulates the proliferation, migration and invasion of non-small cell lung cancer cells. Asia Pac J Clin Oncol. 2022;18(2):39–45.
  • 18. Ma Q, Lu Y, Lin J, Gu Y. ENKUR acts as a tumor suppressor in lung adenocarcinoma cells through PI3K/Akt and MAPK/ERK signaling pathways. J Cancer 2019;10(17):3975–3984.
  • 19. Jain K, Basu A. Multifunctional protein kinase C-ε in cancer development and progression. Cancers 2014 6:860 – 878.
There are 19 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Original Research
Authors

Hilal Balta 0000-0003-3745-9694

Nevin Kocaman 0000-0002-6682-6345

Özlem Üçer 0000-0003-1877-7267

Publication Date March 22, 2023
Acceptance Date March 15, 2023
Published in Issue Year 2023

Cite

AMA Balta H, Kocaman N, Üçer Ö. New proteins in the differentiation of papillary renal cell carcinoma from clear cell renal cell carcinomas; Importance of DARS2, Reelin and Enkurin. J Contemp Med. March 2023;13(2):326-330. doi:10.16899/jcm.1255264