Evaluation of the relationship between vitamin B12 levels and whole blood parameters in children

Abstract

Introduction: Vitamin B12 is a vitamin that can not be produced in the human body, and it could be taken only with animal-sourced foods. Vitamin B12 plays an important role in DNA synthesis, and in its deficiency, DNA synthesis is disrupted. As a result of this, megaloblastic anemia develops. Then later, conditions such as psychiatric, neurological and hematological disorders may occur. In our study, we aimed to determine the relationship between vitamin B12 levels and whole blood parameters in patients between 2-12 years old who have no known chronic disease, and no history of chronic drug use, who applied to our clinic. Methods: This study included 98 patients who were evaluated for vitamin B12 level between 2-12 years olds, and it was carried out in Gülhane Training and Research Hospital pediatric hematology clinic between January 2016 and December 2019. The patients were divided into two groups as normal vitamin B12 level (vitamin B12 level ≥200 pg/ml; Group 1) and vitamin B12 deficiency (serum vitamin B12 level <200 pg/ml; Group 2). Results: Vitamin B12 levels of Group 1 and 2 were found as 311±43.5 and 104.8±15.7, respectively. (p <0.05), (Table). No statistically significant difference was found between white blood cell and platelet counts of groups 1 and 2. White blood cell counts of groups are 7.6±4.1; 6.9±3.5, respectively, and platelet counts of groups are 273.5±74.6; 267.6±95.2, respectively (p >0.05), (Table). Discussion and Conclusion: As a result, vitamin B12 deficiency is frequently encountered in our society. White blood cells, platelet, RDW, MPV, and MCV values, which are full blood parameters, are not sufficient in diagnosis in many cases. In patients suspected of vitamin B12 deficiency, the diagnosis should be checked by measuring the serum vitamin B12 level.

Keywords

• MCV,MPV,RDW,vitamin B12,whole blood parameters

Çocuklarda vitamin B12 düzeyleri ile tam kan parametreleri arasındaki ilişkinin değerlendirilmesi

Abstract

Amaç: Vitamin B12 insan vücudunda üretilemeyen ve sadece hayvansal gıdalar ile alınan bir vitamindir. Vitamin B12 DNA sentezinde önemli rol oynar ve eksikliğinde DNA sentezi bozulur. Sonuç olarak megaloblastik anemi gelişir. Bunun sonucunda psikiyatrik, nörolojik ve hematolojik birçok bozukluk meydana gelebilir. Çalışmamızda kliniğimize başvuran bilinen kronik hastalığı ve herhangi bir kronik ilaç kullanım öyküsü olmayan 2-12 yaş arası hastalarda vitamin B12 düzeyleri ile tam kan parametreleri arasındaki ilişkiyi belirlemeyi amaçladık. Gereç ve Yöntem: Çalışmaya Ocak 2016 ile Aralık 2019 tarihleri arasında Gülhane Eğitim ve Araştırma Hastanesi çocuk hematolojisi polikliniğine başvuran, vitamin B12 düzeyi bakılmış 2-12 yaş arası 98 hasta dahil edildi. Hastalar vitamin B12 düzeyi normal (vitamin B12 düzeyi ≥200 pg/ml; Grup 1) olan ve vitamin B12 eksikliği (serum vitamin B12 düzeyi <200 pg/ml; Grup 2) olan olarak iki gruba ayrıldı. Bulgular: Grup 1 ve 2 nin vitamin B12 düzeyi sıra ile 311±43.5; 104.8±15.7 olarak bulundu (p <0.05), (Tablo). Grup 1 ve 2 nin beyaz küre ve trombosit sayıları arasında istatistisel olarak bir fark bulunmadı, sıra ile beyaz küre sayıları 7.6±4.1; 6.9±3.5, sıra ile trombosit sayıları 273.5±74.6; 267.6±95.2 (p >0.05), (Tablo). Sonuç: Sonuç olarak vitamin B12 eksikliğine toplumumuzda sık olarak karşılaşılmaktadır. Tam kan parametreleri olan beyaz küre, trombosit, RDW, MPV ve MCV değerleri birçok durumda tanıda yeterli olmamaktadır. Vitamin B12 düşüklüğünden şüphelenilen hastalarda mutlaka tanı serum vitamin B12 düzeyi ölçülerek kontrol edilmelidir.

Keywords

• MCV,MPV,RDW,tam kan parametreleri,vitamin B12

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