Research Article
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Komplikasyonsuz Kronik Otitis Media’da Thiol/Disülfit Dengesi

Year 2019, Volume: 9 Issue: 2, 119 - 122, 30.06.2019
https://doi.org/10.16899/jcm.571633

Abstract

Giriş:
Kronik mukozal
inflamatuar bir hastalık olan kronik otitis media patogenezinde oksidatif
stresin rol oynayabileceğini düşündük. Bu amaçla yeni bir oksidatif stres
belirteci ile bu durumu araştırmayı amaçladık.

Gereç
ve yöntemler:
Kronik
ototis media tanısı konulan 30 hasta ve sağlıklı gönüllülerden oluşan 30 birey
kontrol grubu olarak çalışmaya dahil edildi. Kan örnekleri hastaneye yatışta
ameliyat öncesinde alındı. Kontrol grubundaki kan örnekleri polikliniğimize
başvuru esnasında alındı. Thiol/disülfit düzeyleri Erel ve Neşelioğlu
tarafından geliştirilen yeni bir yöntemle analiz edildi.

Sonuçlar:
Kronik otitis
media grubuna 30 birey (20 kadın, 10 erkek) dahil edildi. Konrol grubu olarak da
30 birey (8 kadın, 22 erkek) alındı. Gruplar içerindeki cinsiyet dağılımındaki
farklılık istatistiksel olarak anlamlı (p=0.004) iken yaş dağılımı açısından
anlamlı farklılık yoktu(p=0.072). Ölçülen native thiol disülfit ve total thiol
değerleri açısından gruplar arasında anlamlı farlılık yoktu.







Sonuç:Bu çalışma, kronik otitis medialı
hastalarda thiol oksidasyonunun bir sonucu olarak dinamik thiol disülfit
dengesi disülfit formasyonuna doğru kaydığını göstermesine rğmen gruplar
arasında anlamlı bir farklılık bulunmadı.

References

  • 1. Semaan MT, and Megerian CA. The pathophysiology of cholesteatoma. Otolaryngol Clin North Am 2006; 39:1143-1159.
  • 2. Stool SE BA, Berman S, et al. Otitis Media with Effusion in Young Children. Clinical Practice Guideline. In Rockville, MD: Agency for Health Care Policy and Research, Public Health Service, US Department of Health and Human Services, 1994.
  • 3. Paap CM. Management of otitis media with effusion in young children. Ann Pharmacother 1996; 30:1291-1297.
  • 4. Yilmaz T, Kocan EG, Besler HT, et al. The role of oxidants and antioxidants in otitis media with effusion in children. Otolaryngol Head Neck Surg 2004; 131:797-803.
  • 5. Baysal E, Aksoy N, Kara F, et al. Oxidative stress in chronic otitis media. Eur Arch Otorhinolaryngol 2013; 270:1203-1208.
  • 6. Sen CK, and Packer L. Thiol homeostasis and supplements in physical exercise. Am J Clin Nutr 2000; 72:653S-669S.
  • 7. Cremers CM, and Jakob U. Oxidant sensing by reversible disulfide bond formation. J Biol Chem 2013; 288:26489-26496.
  • 8. Jones DP, and Liang Y. Measuring the poise of thiol/disulfide couples in vivo. Free Radic Biol Med 2009; 47:1329-1338.
  • 9. Biswas S, Chida AS, and Rahman I. Redox modifications of protein-thiols: emerging roles in cell signaling. Biochem Pharmacol 2006; 71:551-564.
  • 10. Circu ML, and Aw TY. Reactive oxygen species, cellular redox systems, and apoptosis. Free Radic Biol Med 2010; 48:749-762.
  • 11. Erel O, and Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem 2014; 47:326-332.
  • 12. Matteucci E, and Giampietro O. Thiol signalling network with an eye to diabetes. Molecules 2010; 15:8890-8903.
  • 13. Prabhu A, Sarcar B, Kahali S, et al. Cysteine catabolism: a novel metabolic pathway contributing to glioblastoma growth. Cancer Res 2014; 74:787-796.
  • 14. Rodrigues SD, Batista GB, Ingberman M, et al. Plasma cysteine/cystine reduction potential correlates with plasma creatinine levels in chronic kidney disease. Blood Purif 2012; 34:231-237.
  • 15. Kundi H, Ates I, Kiziltunc E, et al. A novel oxidative stress marker in acute myocardial infarction; thiol/disulphide homeostasis. Am J Emerg Med 2015; 33:1567-1571.
  • 16. Winther JR, and Thorpe C. Quantification of thiols and disulfides. Biochim Biophys Acta 2014; 1840:838-846.
  • 17. Ellman G, and Lysko H. A precise method for the determination of whole blood and plasma sulfhydryl groups. Anal Biochem 1979; 93:98-102.
  • 18. P M. Chronic suppurative otitis media. Am Fam Physician 2013; 88:694-696,.
  • 19. Dayasena R, Dayasiri M, Jayasuriya C, et al. Aetiological agents in chronic suppurative otitis media in Sri Lanka. Australas Med J 2011; 4:101-104.
  • 20. Garca MF, Turan M, Avsar B, et al. The evaluation of oxidative stress in the serum and tissue specimens of patients with chronic otitis media. Clin Exp Otorhinolaryngol 2015; 8:97-101.

Thiol-disulphide homeostasis in noncomplicated chronic otitis media

Year 2019, Volume: 9 Issue: 2, 119 - 122, 30.06.2019
https://doi.org/10.16899/jcm.571633

Abstract

Introduction: We
hypothesized that oxidative stress plays a role in the pathogenesis of chronic
otitis media, chronic mucosal inflammatory disease. We aimed to investigate a
novel oxidative stress marker in this study.

Materıals and methods: Thirty
patient with chronic otitis media as the patient group and 30 healthy volunteer
subjects as the control group were admitted to the study. Blood samples were taken when they admitted to our clinic
before surgical intervention. In healthy volunteers, blood samples were taken
when they were admitted to our policlinic.   
Thiol/disulphide levels were analyzed with a newly developed method by
Erel and Neselioglu.

Results: 30 subjects were included in chronic otitis media
group (20 females, 10 males).  30
subjects were included in the control group (8 females and 22 males). Sex distribution
within the groups was significantly different (p=0.004). There was no
significant difference between groups with respect to the age distribution
(p=0.072). Measured native thiol, disulphide and total thiol values of the
groups were not significantly different from each other.







Conclusion: This study
demonstrated that although dynamic thiol/disulphide homeostasis was shifted
towards disulphide formation as a result of thiol oxidation in patients with
chronic otitis media. But we could not find any significant difference between
groups.

References

  • 1. Semaan MT, and Megerian CA. The pathophysiology of cholesteatoma. Otolaryngol Clin North Am 2006; 39:1143-1159.
  • 2. Stool SE BA, Berman S, et al. Otitis Media with Effusion in Young Children. Clinical Practice Guideline. In Rockville, MD: Agency for Health Care Policy and Research, Public Health Service, US Department of Health and Human Services, 1994.
  • 3. Paap CM. Management of otitis media with effusion in young children. Ann Pharmacother 1996; 30:1291-1297.
  • 4. Yilmaz T, Kocan EG, Besler HT, et al. The role of oxidants and antioxidants in otitis media with effusion in children. Otolaryngol Head Neck Surg 2004; 131:797-803.
  • 5. Baysal E, Aksoy N, Kara F, et al. Oxidative stress in chronic otitis media. Eur Arch Otorhinolaryngol 2013; 270:1203-1208.
  • 6. Sen CK, and Packer L. Thiol homeostasis and supplements in physical exercise. Am J Clin Nutr 2000; 72:653S-669S.
  • 7. Cremers CM, and Jakob U. Oxidant sensing by reversible disulfide bond formation. J Biol Chem 2013; 288:26489-26496.
  • 8. Jones DP, and Liang Y. Measuring the poise of thiol/disulfide couples in vivo. Free Radic Biol Med 2009; 47:1329-1338.
  • 9. Biswas S, Chida AS, and Rahman I. Redox modifications of protein-thiols: emerging roles in cell signaling. Biochem Pharmacol 2006; 71:551-564.
  • 10. Circu ML, and Aw TY. Reactive oxygen species, cellular redox systems, and apoptosis. Free Radic Biol Med 2010; 48:749-762.
  • 11. Erel O, and Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem 2014; 47:326-332.
  • 12. Matteucci E, and Giampietro O. Thiol signalling network with an eye to diabetes. Molecules 2010; 15:8890-8903.
  • 13. Prabhu A, Sarcar B, Kahali S, et al. Cysteine catabolism: a novel metabolic pathway contributing to glioblastoma growth. Cancer Res 2014; 74:787-796.
  • 14. Rodrigues SD, Batista GB, Ingberman M, et al. Plasma cysteine/cystine reduction potential correlates with plasma creatinine levels in chronic kidney disease. Blood Purif 2012; 34:231-237.
  • 15. Kundi H, Ates I, Kiziltunc E, et al. A novel oxidative stress marker in acute myocardial infarction; thiol/disulphide homeostasis. Am J Emerg Med 2015; 33:1567-1571.
  • 16. Winther JR, and Thorpe C. Quantification of thiols and disulfides. Biochim Biophys Acta 2014; 1840:838-846.
  • 17. Ellman G, and Lysko H. A precise method for the determination of whole blood and plasma sulfhydryl groups. Anal Biochem 1979; 93:98-102.
  • 18. P M. Chronic suppurative otitis media. Am Fam Physician 2013; 88:694-696,.
  • 19. Dayasena R, Dayasiri M, Jayasuriya C, et al. Aetiological agents in chronic suppurative otitis media in Sri Lanka. Australas Med J 2011; 4:101-104.
  • 20. Garca MF, Turan M, Avsar B, et al. The evaluation of oxidative stress in the serum and tissue specimens of patients with chronic otitis media. Clin Exp Otorhinolaryngol 2015; 8:97-101.
There are 20 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Original Research
Authors

Kazım Bozdemir

Bülent Ulusoy

Arife Sezgin This is me

Ahmet Akkoz This is me

Özcan Erel

Mehmet Hakan Korkmaz This is me

Publication Date June 30, 2019
Acceptance Date May 10, 2019
Published in Issue Year 2019 Volume: 9 Issue: 2

Cite

AMA Bozdemir K, Ulusoy B, Sezgin A, Akkoz A, Erel Ö, Korkmaz MH. Thiol-disulphide homeostasis in noncomplicated chronic otitis media. J Contemp Med. June 2019;9(2):119-122. doi:10.16899/jcm.571633