Does the Covid-19 pandemic have an effect on wound culture in patients undergoing appendectomy? A Case Control Study

Year 2022, Volume: 12 Issue: 2, 332 - 338, 15.03.2022

Hacı Bolat , Tuğba Avan Mutlu

https://doi.org/10.16899/jcm.1075112

Abstract

Aim: The aim of this study is to examine the effect of the changing microbiota structure during the pandemic period on the bacterial aerobic culture profile of the wound taken from patients operated for acute appendicitis, together with clinical variables.
Materials and Methods: Our study included 125 patients who underwent an appendectomy in the General Surgery Clinic between 01.03.2019-01.02.2021 and whose wound culture was taken during an appendectomy. The patients were divided into two groups; group1 (pre-pandemic) and group2 (during a pandemic). Both groups were compared in terms of age, gender, clinical, laboratory and wound culture data.
Results: There was no significant difference between the groups in terms of age, clinical symptoms, surgical method, laboratory and radiological data (p>0.05). In the pre-pandemic group, there was growth of E. coli in 35 (53%) patients, K. pneumoniae in 3 (4.5%) patients, and P. aeruginosae in 2 (3) patients from Gram-negative bacteria while Strep. anginosus and Strep. constellatus growth were most common from Gram-positive bacteria. In the pandemic group, there was the growth of E. coli in 29 (49.2%) patients, P. aeruginosae in 5 (8.5%) patients, K. pneumoniae in 2 (3.4%) patients from Gram-negative bacteria, Citrobacterfreundii and Streptococcusanginosus growth were the most common from Gram-positive bacteria. Escherichiacoli is the most common bacteria in both groups.
Conclusion: Although E. coli was found to be the most frequently identified microorganism in patients with acute appendicitis, an increase in the density and resistance of Pseudomonas group bacteria were detected, possibly due to the effect of the COVID-19 pandemic.

Keywords

appendectomy, COVID-19 pandemic, wound culture, antibiotic resistance

Covid-19 Pandemisinin Apendektomi Yapılan Hastalarda Yara Yeri Kültürüne Etkisi Var Mı? Vaka Kontrol Çalışması

Abstract

Amaç: Yaptığımız bu çalışmada amaçpandemi döneminde değişen mikrobiyota yapısının akut apandisit nedeni ile opere edilen hastalardan alınan yara yeri bakteriyel aerobik kültür profilineolan etkisini klinik değişkenlerle birlikte incelemektir.
Gereç ve Yöntem: Çalışmamıza 01.03.2019-01.02.2021 tarihleri arasında Genel Cerrahi Kliniğinde apendektomi yapılan ve apendektomi esnasında yara yeri kültürü alınan 125 hasta dahil edildi. Hastalar iki gruba ayrıldı; grup1(pandemi öncesi) ve grup2 (pandemi süreci). Her iki grup; yaş, cinsiyet, klinik, laboratuvar ve yara yeri kültür verileri açısından karşılaştırıldı.
Bulgular: Gruplar arasında yaş, klinik semptomlar, ameliyat yöntemi, laboratuvar ve radyolojik verileriler açısından anlamlı fark yoktu(p>0,05). Pandemi sürecindeki grupta perfora apendisit, apendokolit, hastanede kalış ve antibiyotik kullanım süresi daha fazla oduğu tespit edildi(p<0,05). Pandemi öncesinde Gram negatif bakterilerden 35 (%53) hastada E. coli, 3 (%4,5) hastada K. pneumoniae, 2 (3) hastada P. aeruginosae üremesi olurken Gram pozitif bakterilerden en çok Strep.anginosusveStrep.constellatusüremesiolmuştur. Pandemidönemindeise Gram negative bakterilerden 29 (%49,2) hastada E. coli, 5 (%8,5) hastada P. aeruginosae, 2 (%3,4) hastada K. pneumoniae üremesi olurken Gram pozitif bakterilerden en çok CitrobacterfreundiiveStreptococcusanginosusüremesiolmuştur. Her ikigrupta da enyaygınüreyenbakteriEscherichiacoli ‘dir.
Sonuç:Akut apandisitli hastalarda en sık tanımlanan mikroorganizma E. coli olarak bulunmuş olsa da muhtemel olarak COVID-19 pandemi sürecinin etkisi ile Pseudomonas gurubu bakterilerin yoğunluğunda ve direnç durumunda artış tespit edilmiştir.

Keywords

COVID-19 Pandemisi, apandektomi, yara yeri kültürü, antibiyotik direnci

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