Western-blot, PCR and immunofluorescence analysis in mitochondrial biogenesis studies

Abstract

Mitochondria are providing an essential amount of energy to the cell, to achieve in homeostasis, metabolic increases, proliferation and differentiation processes. Also, mitochondrial deficiencies have severe or lethal impacts on cell viability. Among the 3000 proteins involved in mitochondrial activities, ATAD3 is a major one as essential for mitochondrial biogenesis, vital as early as embryonic implantation. In order to see its  impact at animal level, we have used ATAD3+/- mice to investigate its role in running training and in high calorie diet. We found here that ATAD3 expression level avoids running capacity improvement and has a strong effect on weight increase, underlying its important role in mitochondrial mass regulations. Prior to this presentation we will emphasize on the potential of Western-blot, PCR and immunofluorescence analysis in biomedical researches.

Keywords

• Mitochondria,ATAD3,Western-blot,PCR,immunofluorescence

References

1. Li S, Bouzar C, Cottet-Rousselle C, Zagotta I, Lamarche F, Wabitsch M, Tokarska-Schlattner M, Fischer-Posovszky P, Schlattner U, Rousseau D.Biochim Biophys Acta. 2016 Jun;1857(6):643-52. doi: 10.1016/j.bbabio.2016.03.009. Epub 2016 Mar 8.