EFFECTS OF OCCUPATIONAL EXPOSURE TO IONIZING RADIATION ON OXIDATIVE STRESS AND INFLAMMATORY MARKERS IN HEALTHCARE WORKERS OF A UNIVERSITY HOSPITAL IN KONYA, TURKEY

Year 2021, Volume: 13 Issue: 2, 994 - 1003, 11.02.2022

Zehra Ardıç , Tahir Kemal Şahin , Mehmet Uyar , Hasan Küçükkendirci , İbrahim Kılınc , Elif Nur Yıldırım Öztürk

https://doi.org/10.37212/jcnos.1012615

Cited By: 2

Abstract

OBJECTIVE Ionizing radiation (IR) has a wide area of use and its effects on human health have been discussed since its discovery. This study aimed to show oxidative stress and inflammation due to ionizing radiation exposure based on biomarkers in healthcare workers.
METHOD This study was conducted with 172 people, who were exposed to IR in the work environment and those who did not have exposure to radiation. In this cross-sectional study, a data collection form was used to obtain data from the participants. In addition, 6 ml of blood was taken to measure their tumor necrosis factor (TNF)-alpha, total oxidant status (TOS), interleukin (IL)-10 and total antioxidant status (TAS) levels, and calculate their oxidative stress index (OSI) values.
RESULTS In the ionizing radiation group, 50% of the participants were men, the mean age was 35.91±7.07 years, and the mean duration of employment was 9.80±7.1 years. The TOS, OSI, TNF-α and IL-10 values were higher and TAS was lower in the ionizing radiation group compared to the participants without exposure to ionizing radiation. Gender, smoking, alcohol use, presence of chronic diseases, regular medication use, antioxidant supplement use, and exposure to radiation for medical diagnosis and treatment within the last year did not affect oxidative stress and inflammation in the radiation workers. The cut-off values of the TOS, TAS, OSI, TNF-α and IL-10 biomarkers were also determined.
CONCLUSION Occupational low-dose long-term exposure to ionizing radiation was found to increase oxidative stress and inflammation.

Keywords

ionizing radiation, oxidative stress, TNF-α, interleukin-10, occupational exposure

Supporting Institution

Necmettin Erbakan University Scientific Research Projects Coordinatorship

Project Number

201518006

References

• 1. Ahmad IM, Abdalla MY, Moore TA, Bartenhagen L, Case AJ, Zimmerman MC. Healthcare Workers Occupationally Exposed to Ionizing Radiation Exhibit Altered Levels of Inflammatory Cytokines and Redox Parameters. Antioxidants (Basel). 2019;8(1):12.
• 2. Ahmad IM, Temme JB, Abdalla MY, Zimmerman MC. Redox status in workers occupationally exposed to long-term low levels of ionizing radiation: A pilot study. Redox Rep. 2016;21(3):139-45.
• 3. Akköse A, Ömer B, Yiğitbaşı A. DNA damage and glutathione content in radiology technicians. ClinicaChimicaActa. 2003;336:13-18.
• 4. Aneva N, Zaharieva E, Katsarska O, Savova G, Stankova K, Djounova J, Boteva R. Inflammatory profile dysregulation in nuclear workers occupationally exposed to low-dose gamma radiation. Journal of radiation research. 2019;60(6):768-779.
• 5. Arslan N. The Effects of Radiation on Biological Systems.Nucl Med Semin. 2017;3:178-183.
• 6. Azzam EI, Jay-Gerin JP, Pain D. Ionizing radiation-induced metabolic oxidative stress and prolonged cell injury. Cancer Lett. 2012;327(1-2):48-60.
• 7. Çelik S. Preparation of the radiation safety programme - optimization of the radiation protection of the nuclear sciences institute of University of Ankara (master’s thesis). Ankara, University of Ankara, 2013.
• 8. Chen B, Dai Q, Zhang Q, Yan P, Wang A, Qu L, Jin Y, Zhang D. The relationship among occupational irradiation, DNA methylation status, and oxidative damage in interventional physicians.Medicine (Baltimore). 2019;98(39):e17373.
• 9. Dawson-Saunders B, Trapp RG. Basic and Clinical Biostatistics. Norwalk, Appleton & Lange, 1990.
• 10. Durović B, Spasić-Jokić V, Durović B. Influence of occupational exposure to low-dose ionizing radiation on the plasma activity of superoxide dismutase and glutathione level. Vojnosanit Pregl. 2008;65(8):613-8.
• 11. Eken A, Aydin A, Erdem O, Akay C, Sayal A, Somuncu I. Induced antioxidant activity in hospital staff occupationally exposed to ionizing radiation. Int J Radiat Biol. 2012;88(9):648-53.
• 12. El-Benhawy SA, El-Tahan RA, Nakhla SF. Exposure to Radiation During Work Shifts and Working at Night Act as Occupational Stressors Alter Redox and Inflammatory Markers. Arch Med Res. 2020;7:S0188-4409(19)30919-1.
• 13. Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. ClinBiochem. 2004;37:112-9.
• 14. Erel O. A new automated colorimetric method for measuring total oxidant status. ClinBiochem. 2005;38:1103-11.
• 15. Faul F, Erdfelder E, Buchner A, Lang AG. Statistical power analyses using G* Power 3.1: Tests for correlation and regression analyses. Behavior research methods. 2009;41(4):1149-1160.
• 16. Faul F, Erdfelder E, Lang AG, Buchner A. G* Power 3: A flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behavior research methods. 2007;39(2):175-191.
• 17. Fazel R, Einstein AJ. Radiation risk to healthcare workers from diagnostic and interventional imaging procedures. Cutlip D, Windecker S, Estes NAM, Saperia GM, ed. UpToDate. Waltham, MA: UpToDate Inc.https://www.uptodate.com (accessed 2020 July 21)
• 18. Gao J, Dong X, Liu T, Zhang L, Ao L. Antioxidant status and cytogenetic damage in hospital workers occupationally exposed to low dose ionizing radiation. Mutat Res. 2020;850-851:503152.
• 19. Heidari S, Taheri M, Ravan AP, Moghimbeigi A, Mojiri M, NaderiKhojastehfar Y, Paydari-Banyarani S, Hassanpour Z, Eftekharian MM. Assessment of some immunological and hematological factors among radiation workers. J Biol Today's World. 2016;5:113–9.
• 20. Katsarska O, Zaharieva E, Aneva N, Savova G, Stankova K, Boteva R. The soluble receptor ST2 is positively associated with occupational exposure to radiation. Int J Radiat Biol. 2016;92(2): 87-93.
• 21. Kayan M, Nazıroğlu M, Çelik Ö, Yalman K, Köylü H. Vitamin C and E combination modulates oxidative stress induced by X-ray in blood of smoker and nonsmoker radiology technicians. Cell Biochemistry and Function. 2009;27:424–429.
• 22. Kłuciński P, Wójcik A, Grabowska-Bochenek R, Gmiński J, Mazur B, Hrycek A, Cieślik P, Martirosian G. Erythrocyte antioxidant parameters in workers occupatıonally exposed to low levels of ionizing radiatıon. Ann Agric Environ Med. 2008;15:9–12.
• 23. Kumar D, Kumari S, Salian SR, Uppangala S, Kalthur G, Challapalli S, Chandraguthi SG, Kumar P, Adiga SK. Genetic Instability in Lymphocytes is Associated With Blood Plasma Antioxidant Levels in Health Care Workers Occupationally Exposed to Ionizing Radiation. Int J Toxicol. 2016;35(3): 327-35.
• 24. Malekirad AA, Ranjbar A, Rahzani K, Pilehvarian AA, Rezaie A, Zamani MJ, Abdollahi M. Oxidative stress in radiology staff. Environ ToxicolPharmacol. 2005;20(1): 215-8.
• 25. Mons U, Muscat JE, Modesto J, Richie JP Jr, Brenner H. Effect of smoking reduction and cessation on the plasma levels of the oxidative stress biomarker glutathione Post-hoc analysis of data from a smoking cessation trial. Free Radic Biol Med. 2016;91:172-7.
• 26. Najafi M, Motevaseli E, Shirazi A, Geraily G, Rezaeyan A, Norouzi F, Rezapoor S, Abdollahi H. Mechanisms of inflammatory responses to radiation and normal tissues toxicity: clinical implications. International Journal of Radiation Biology. 2018;94(4):335-356.
• 27. Pisoschi AM, Pop A. The role of antioxidants in the chemistry of oxidative stress: A review. Eur J Med Chem. 2015;5(97):55-74.
• 28. Radiation: effects and sources. United Nations Environment Programme (UNEP). ISBN: 978-92-807-3517-8. Job No.: DEW/1937/NA, 2016.
• 29. Roh JS, Sohn DH. Damage-associated molecular patterns in ınflammatory diseases. Immune Netw. 2018;18(4):e27.
• 30. Sebastià N, Olivares-González L, Montoro A, Barquinero JF, Canyada-Martinez AJ, HervásD,Gras P, Villaescusa JI, Marti-Bonmati L, Muresan BT,et al. Redox status, dose and antioxidant ıntake in healthcare workers occupationally exposed to ıonizing radiation. Antioxidants (Basel). 2020;9(9):778.
• 31. Selek S, Aslan M, Horoz M, Gur M, Erel O. Oxidative status and serum PON1 activity in beta-thalassemia minor.ClinBiochem. 2007;40: 287-91.
• 32. Siama Z, Zosang-Zuali M, Vanlalruati A, Jagetia GC, Pau KS, Kumar NS. Chronic low dose exposure of hospital workers to ionizing radiation leads to increased micronuclei frequency and reduced antioxidants in their peripheral blood lymphocytes. Int J Radiat Biol. 2019;95(6):697-709.
• 33. Tokaç D. Assessment of changes in the oxidative stress parameters induced by occupational exposure in welders [dissertation]. Ankara, University of Hacettepe, 2018.
• 34. Yahyapour R, Amini P, Rezapour S, Cheki M, Rezaeyan A, Farhood B, Shabeeb D, Musa AE, Fallah H, Najafi M. Radiation-induced inflammation and autoimmune diseases. Mil Med Res. 2018;5(1): 9.
• 35. Yanbaeva DG, Dentener MA, Creutzberg EC, Wesseling G, Wouters EF. Systemic effects of smoking. Chest. 2007,131(5):1557-66.
• 36. Zakeri F, Hirobe T, AkbariNoghabi K. Biological effects of low-dose ionizing radiation exposure on interventional cardiologists .Occup Med (Lond). 2010;60(6):464-9.