ANTIBACTERIAL EFFECTS OF PARMELIA SULCATA AND HYPOGYMNIA TUBULOSA ACETONE EXTRACTS AGAINST ISOLATES FROM SOAK LIQUORS

Year 2020, , 251 - 257, 01.09.2020

Didem Berber

https://doi.org/10.7240/jeps.633056

Abstract

Abstract

It is well known that there are halophilic
or non-halophilic bacteria in salt, soak liquors, salted and soaked hides/skins
in high numbers in leather industry. These bacteria have several hydrolytic
enzymes which cause irreversible defects on finished leather product. However,
antimicrobial agents are utilized to control bacterial population in soaking
process, these agents have not sufficient efficacy due to inadequate
application of these agents or the presence of antimicrobial-resistant
bacterial strains in soak liquors. In this respect, alternative agents or
strategies may be helpful for controlling bacterial population. For this
purpose, ten isolates were obtained from different soak liquor samples and the
antibacterial effects of acetone extracts of Parmelia sulcata and Hypogymnia
tubulosa lichen species were evaluated on these isolates. Colony
morphologies on agar plates, Gram staining, oxidase and catalase tests,
bacterial growth on several selective media of these isolates were
investigated. They were Gram-positive,
rod-shaped and catalase positive. While bacterial growth was observed on
Manntiol Salt Agar, Baird Parker agar, 5% sheep blood agar in most isolates, no
growth was detected on Eosine-methylene blue agar and Cetrimide agar.
Additionally, antibacterial activities for acetone
extracts of Parmelia sulcata and Hypogymnia tubulosa against these
isolates were tested at certain concentrations of 240, 120, 60 and 30 µg/ml. Hypogymnia tubulosa extracts were found to be more successful in
comparison to the extracts of Parmelia
sulcata on tested isolates. These tested lichen species can be used
to control the population of bacteria in the soaking process and also to
prevent potential defects on the skin that may be seen in subsequent tanning
processes due to the development of these bacteria.

Keywords

soak liquor, lichen, Parmelia sulcata, Hypogymnia tubulosa, acetone extract, antibacterial

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