Year 2020, Volume 32 , Issue 4, Pages 499 - 506 2020-11-01

Infusion or Decoction Extracts of Helianthus annuus Leaves: Potential Inhibitors for QS system and Biofilm Formation in Pseudomonas aeruginosa
Helianthus annuus Yapraklarının İnfüzyon veya Dekoksiyon Özütleri: Pseudomonas aeruginosa’nın QS Sistemi ve Biyofilm Oluşumu Üzerine Potansiyel İnhibitörler

Arhun Ali BALKAN [1] , Ayla YILDIZ [2] , Didem BERBER [3] , Barış GÖKALSIN [4] , Cenk SESAL [5]


Pseudomonas aeruginosa is one of the drug-resistant opportunistic pathogens with the ability to form biofilm and to produce a number of virulence factors via Quorum Sensing (QS) regulation. Most researchers have focused on QS inhibition to overcome the drug resistance problem. QS inhibitor molecules are investigated from natural resources. In the present study, anti-QS activities of ethyl acetate extracts of decoction and infusion samples from Helianthus annuus leaves were tested on biosensor strains of P. aeruginosa (lasB-gfp, rhlA-gfp and pqsA-gfp), as well as anti-biofilm activities on PAO1 wild type. H. annuus leaf samples were firstly infused or decocted and then extracted with ethyl acetate. The efficacies of infusion or decoction extracts were examined at the concentrations of 240, 120, and 60 μg/ml in 96-well microplates and evaluated in Citation 3 multimode microplate reader (Biotek). The inhibition rates of decoction extracts were recorded as 70.61% for las, 44.09% for rhl and 83.77% pqs system at 240 μg/ml. The biofilm inhibition percentages of the extracts were determined to be 50.82% (±1.36). Moreover, inhibition rates for infusion extracts were detected as 62.08% for las, 45.15% for rhl and 77.79% for pqs, and 53.88% (±3.94) for biofilm formation. In conclusion, the potential efficacies of the extracts of decocted or infused H.annuus leaves were demonstrated on QS system and biofilm formation of P. aeruginosa. However, there is a need for more detailed investigations and determination of the active substances that have QSI and anti-biofilm effect.
Pseudomonas aeruginosa is one of the drug-resistant opportunistic pathogens with the ability to form biofilm and to produce a number of virulence factors via Quorum Sensing (QS) regulation. Most researchers have focused on QS inhibition to overcome the drug resistance problem. QS inhibitor molecules are investigated from natural resources. In the present study, anti-QS activities of ethyl acetate extracts of decoction and infusion samples from Helianthus annuus leaves were tested on biosensor strains of P. aeruginosa (lasB-gfp, rhlA-gfp and pqsA-gfp), as well as anti-biofilm activities on PAO1 wild type. H. annuus leaf samples were firstly infused or decocted and then extracted with ethyl acetate. The efficacies of infusion or decoction extracts were examined at the concentrations of 240, 120, and 60 μg/ml in 96-well microplates and evaluated in Citation 3 multimode microplate reader (Biotek). The inhibition rates of decoction extracts were recorded as 70.61% for las, 44.09% for rhl and 83.77% pqs system at 240 μg/ml. The biofilm inhibition percentages of the extracts were determined to be 50.82% (±1.36). Moreover, inhibition rates for infusion extracts were detected as 62.08% for las, 45.15% for rhl and 77.79% for pqs, and 53.88% (±3.94) for biofilm formation. In conclusion, the potential efficacies of the extracts of decocted or infused H.annuus leaves were demonstrated on QS system and biofilm formation of P. aeruginosa. However, there is a need for more detailed investigations and determination of the active substances that have QSI and anti-biofilm effect.
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Primary Language en
Subjects Science, Basic Sciences
Journal Section Research Articles
Authors

Orcid: 0000-0002-8836-7469
Author: Arhun Ali BALKAN
Institution: MARMARA ÜNİVERSİTESİ, FEN BİLİMLERİ ENSTİTÜSÜ, BİYOLOJİ (YL) (TEZLİ)
Country: Turkey


Orcid: 0000-0002-8017-4080
Author: Ayla YILDIZ
Institution: MARMARA ÜNİVERSİTESİ, FEN BİLİMLERİ ENSTİTÜSÜ, BİYOLOJİ (YL) (TEZLİ)
Country: Turkey


Orcid: 0000-0001-5813-160X
Author: Didem BERBER
Institution: MARMARA ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ
Country: Turkey


Orcid: 0000-0001-5060-6834
Author: Barış GÖKALSIN
Institution: MARMARA ÜNİVERSİTESİ, FEN BİLİMLERİ ENSTİTÜSÜ, BİYOLOJİ (DR)
Country: Turkey


Orcid: 0000-0002-0737-0122
Author: Cenk SESAL (Primary Author)
Institution: MARMARA ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ
Country: Turkey


Dates

Publication Date : November 1, 2020

Bibtex @research article { jeps741137, journal = {International Journal of Advances in Engineering and Pure Sciences}, issn = {}, eissn = {2636-8277}, address = {fbedergi@marmara.edu.tr}, publisher = {Marmara University}, year = {2020}, pages = {499 - 506}, doi = {10.7240/jeps.741137}, title = {Infusion or Decoction Extracts of Helianthus annuus Leaves: Potential Inhibitors for QS system and Biofilm Formation in Pseudomonas aeruginosa}, key = {cite}, author = {Balkan, Arhun Ali and Yıldız, Ayla and Berber, Didem and Gökalsın, Barış and Sesal, Cenk} }
APA Balkan, A , Yıldız, A , Berber, D , Gökalsın, B , Sesal, C . (2020). Infusion or Decoction Extracts of Helianthus annuus Leaves: Potential Inhibitors for QS system and Biofilm Formation in Pseudomonas aeruginosa . International Journal of Advances in Engineering and Pure Sciences , 32 (4) , 499-506 . DOI: 10.7240/jeps.741137
MLA Balkan, A , Yıldız, A , Berber, D , Gökalsın, B , Sesal, C . "Infusion or Decoction Extracts of Helianthus annuus Leaves: Potential Inhibitors for QS system and Biofilm Formation in Pseudomonas aeruginosa" . International Journal of Advances in Engineering and Pure Sciences 32 (2020 ): 499-506 <https://dergipark.org.tr/en/pub/jeps/article/741137>
Chicago Balkan, A , Yıldız, A , Berber, D , Gökalsın, B , Sesal, C . "Infusion or Decoction Extracts of Helianthus annuus Leaves: Potential Inhibitors for QS system and Biofilm Formation in Pseudomonas aeruginosa". International Journal of Advances in Engineering and Pure Sciences 32 (2020 ): 499-506
RIS TY - JOUR T1 - Infusion or Decoction Extracts of Helianthus annuus Leaves: Potential Inhibitors for QS system and Biofilm Formation in Pseudomonas aeruginosa AU - Arhun Ali Balkan , Ayla Yıldız , Didem Berber , Barış Gökalsın , Cenk Sesal Y1 - 2020 PY - 2020 N1 - doi: 10.7240/jeps.741137 DO - 10.7240/jeps.741137 T2 - International Journal of Advances in Engineering and Pure Sciences JF - Journal JO - JOR SP - 499 EP - 506 VL - 32 IS - 4 SN - -2636-8277 M3 - doi: 10.7240/jeps.741137 UR - https://doi.org/10.7240/jeps.741137 Y2 - 2020 ER -
EndNote %0 International Journal of Advances in Engineering and Pure Sciences Infusion or Decoction Extracts of Helianthus annuus Leaves: Potential Inhibitors for QS system and Biofilm Formation in Pseudomonas aeruginosa %A Arhun Ali Balkan , Ayla Yıldız , Didem Berber , Barış Gökalsın , Cenk Sesal %T Infusion or Decoction Extracts of Helianthus annuus Leaves: Potential Inhibitors for QS system and Biofilm Formation in Pseudomonas aeruginosa %D 2020 %J International Journal of Advances in Engineering and Pure Sciences %P -2636-8277 %V 32 %N 4 %R doi: 10.7240/jeps.741137 %U 10.7240/jeps.741137
ISNAD Balkan, Arhun Ali , Yıldız, Ayla , Berber, Didem , Gökalsın, Barış , Sesal, Cenk . "Infusion or Decoction Extracts of Helianthus annuus Leaves: Potential Inhibitors for QS system and Biofilm Formation in Pseudomonas aeruginosa". International Journal of Advances in Engineering and Pure Sciences 32 / 4 (November 2020): 499-506 . https://doi.org/10.7240/jeps.741137
AMA Balkan A , Yıldız A , Berber D , Gökalsın B , Sesal C . Infusion or Decoction Extracts of Helianthus annuus Leaves: Potential Inhibitors for QS system and Biofilm Formation in Pseudomonas aeruginosa. JEPS. 2020; 32(4): 499-506.
Vancouver Balkan A , Yıldız A , Berber D , Gökalsın B , Sesal C . Infusion or Decoction Extracts of Helianthus annuus Leaves: Potential Inhibitors for QS system and Biofilm Formation in Pseudomonas aeruginosa. International Journal of Advances in Engineering and Pure Sciences. 2020; 32(4): 499-506.
IEEE A. Balkan , A. Yıldız , D. Berber , B. Gökalsın and C. Sesal , "Infusion or Decoction Extracts of Helianthus annuus Leaves: Potential Inhibitors for QS system and Biofilm Formation in Pseudomonas aeruginosa", International Journal of Advances in Engineering and Pure Sciences, vol. 32, no. 4, pp. 499-506, Nov. 2020, doi:10.7240/jeps.741137