Kemik defektlerinin tedavisi için β-trikalsiyum fosfat ile birleştirilmiş aljinat ve yumurta kabuğu zarı içeren biyomateryalin değerlendirilmesi

Year 2025, Volume: 37 Issue: 3, 293 - 298, 24.09.2025

Semra Unal

https://doi.org/10.7240/jeps.1703374

Abstract

Kemik defektlerinin tedavisi için tasarlanan iskele, dondurarak kurutma yöntemi kullanılarak üretilmiştir. İskelelerin karakterizasyonu fourier transform infrared (FTIR) spektroskopisi ve X-ışını kırınım analizi, şişme ve bozunma oranı, taramalı elektron mikroskobu (SEM) testleri kullanılarak gerçekleştirilmiştir. İskelelerin biyouyumluluğunu analiz etmek için osteoblast hücreleri ile in vitro sitotoksisite deneyi yapılmıştır. Karakterizasyon sonuçları aljinat, yumurta kabuğu membranı ve β-TCP arasında güçlü iyonik etkileşimler ve birbirine bağlı gözenekli yapı olduğunu göstermiştir. Hücre canlılığının %80'den fazla olması iskelelerin toksik olmadığını göstermektedir. Tüm sonuçlar birlikte değerlendirildiğinde, aljinat/yumurta kabuğu zarı içeren β-TCP'nin kemik tedavisi uygulamaları için uygun olduğu düşünülebilir.

Keywords

aljinat , yumurta kabuğu zarı , beta-trikalsiyum fosfat , doku mühendisliği

Evaluation of biomaterial containing alginate and egg-shell membrane incorporated with β-tricalcium phosphate for treatment of bone defects

Abstract

The freeze-drying procedure was used to create the scaffold that was meant to cure bone deformities. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction analysis, swelling and degradation ratio, and scanning electron microscopy (SEM) tests were used to characterize the scaffolds. Osteoblast cells were used in an in vitro cytotoxicity test to evaluate the scaffolds' biocompatibility. The results of the characterization revealed an interconnected porous structure and significant ionic connections between the alginate, egg-shell membrane, and β-tricalcium phosphate (β-TCP). The scaffolds are regarded as nontoxic because the cell viability was more than 80%. When all the findings are evaluated, β-TCP with alginate/egg-shell membrane can be regarded as appropriate for bone treatment applications.

Keywords

alginate , egg shell membrane , beta-tricalcium phosphate , tissue engineering

References

• Cakmak, A.M., Unal, S., Sahin, A., Oktar, F.N., Sengor, M., Ekren, N., Gunduz, O., & Kalaskar, D.M. (2020). 3D printed polycaprolactone/gelatin/bacterial cellulose/hydroxyapatite composite scaffold for bone tissue engineering. Polymers (Basel), 12, 1–14. doi:10.3390/polym12091962.
• Silva-Barroso, A.S., Cabral, C.S.D., Ferreira, P., Moreira, A.F., & Correia, I.J. (2023). Lignin-enriched tricalcium phosphate/sodium alginate 3D scaffolds for application in bone tissue regeneration. International Journal of Biological Macromolecules, 239. doi:10.1016/j.ijbiomac.2023.124258.
• Lin, X., Patil, S., Gao, Y.G., & Qian, A. (2020). The Bone Extracellular Matrix in Bone Formation and Regeneration. Frontiers in Pharmacology, 11, 1–15. doi:10.3389/fphar.2020.00757.
• Garimella, A., Ghosh, S.B., & Bandyopadhyay-Ghosh, S. (2024). Biomaterials for bone tissue engineering: achievements to date and future directions. Biomedical Materials, 20. doi:10.1088/1748-605X/ad967c.
• Jia, J., Duan, Y.Y., Yu, J., & Lu, J.W. (2008). Preparation and immobilization of soluble eggshell membrane protein on the electrospun nanofibers to enhance cell adhesion and growth. Journal of Biomedical Materials Research - Part A, 86, 364–373. doi:10.1002/jbm.a.31606.
• Farjah, G.H., Mohammdzadeh, S., & Javanmard, M.Z. (2020). The effect of lycopene in egg shell membrane guidance channel on sciatic nerve regeneration in rats. Iranian Journal of Basic Medical Sciences, 23, 527–533. doi:10.22038/ijbms.2020.40228.9525.
• Adali, T., Kalkan, R., & Karimizarandi, L. (2019). The chondrocyte cell proliferation of a chitosan/silk fibroin/egg shell membrane hydrogels. International Journal of Biological Macromolecules, 124, 541–547. doi:10.1016/j.ijbiomac.2018.11.226.
• Li, X., Cai, Z., Ahn, D.U., & Huang, X. (2019). Development of an antibacterial nanobiomaterial for wound-care based on the absorption of AgNPs on the eggshell membrane. Colloids and Surfaces B: Biointerfaces, 183, 110449. doi:10.1016/j.colsurfb.2019.110449.
• Ocando, C., Dinescu, S., Samoila, I., Ghitulica, C.D., Cucuruz, A., Costache, M., & Averous, L. (2021). Fabrication and properties of alginate-hydroxyapatite biocomposites as efficient biomaterials for bone regeneration. European Polymer Journal, 151, 110444. doi:10.1016/j.eurpolymj.2021.110444.
• Afriani, F., Dahlan, K., Nikmatin, S., & Zuas, O. (2015). Alginate Affecting The Characteristics Of Porous Beta-Tcp/Alginate Composite Scaffolds. Journal of Optoelectronics and Biomedical Materials. https://publons.com/publon/31759775/.
• Liang, L., Hou, T., Ouyang, Q., Xie, L., Zhong, S., Li, P., Li, S., & Li, C. (2020). Antimicrobial sodium alginate dressing immobilized with polydopamine-silver composite nanospheres. Composites Part B: Engineering, 188, 107877. doi:10.1016/j.compositesb.2020.107877.
• Mohammadzadeh, L., Rahbarghazi, R., Salehi, R., & Mahkam, M. (2019). A novel egg-shell membrane based hybrid nanofibrous scaffold for cutaneous tissue engineering. Journal of Biological Engineering, 13, 1–15. doi:10.1186/s13036-019-0208-x.
• Guler, E., Baripoglu, Y.E., Alenezi, H., Arikan, A., Babazade, R., Unal, S., Duruksu, G., Alfares, F.S., Yazir, Y., Oktar, F.N., Gunduz, O., Edirisinghe, M., & Cam, M.E. (2021). Vitamin D3/vitamin K2/magnesium-loaded polylactic acid/tricalcium phosphate/polycaprolactone composite nanofibers demonstrated osteoinductive effect by increasing Runx2 via Wnt/B-catenin pathway. International Journal of Biological Macromolecules. doi:10.1016/j.ijbiomac.2021.08.196.
• Algul, D., Sipahi, H., Aydin, A., Kelleci, F., Ozdatli, S., & Yener, F.G. (2015). Biocompatibility of biomimetic multilayered alginate-chitosan/β-TCP scaffold for osteochondral tissue. International Journal of Biological Macromolecules, 79, 363–369. doi:10.1016/j.ijbiomac.2015.05.005.
• Ghaffari, R., Salimi-Kenari, H., Fahimipour, F., Rabiee, S.M., Adeli, H., & Dashtimoghadam, E. (2020). Fabrication and characterization of dextran/nanocrystalline β-tricalcium phosphate nanocomposite hydrogel scaffolds. International Journal of Biological Macromolecules, 148, 434–448. doi:10.1016/j.ijbiomac.2020.01.112.