PRODUCTION AND CHARACTERIZATION OF BACTERIAL CELLULOSE BIOCOMPOSITES BASED ON THYMUS SIPYLEUS BOISS. EXTRACT

Abstract

The aim of this study was to improve the antibacterial properties of bacterial cellulose (BC) produced by ex situ modification in Kombucha culture medium. Bioactive methanol extract from the plant Thymus sipyleus Boiss. was added to BC by ex situ modification to obtain a bioactive and cost-effective biocomposite. SEM and FTIR analyses confirmed the nanofibers, porous structure and chemical bonding of the extract with the BC nanofibers and revealed that the biocomposites were successfully produced. A significant decrease in water retention and swelling behavior was observed in the biocomposites. The antibacterial activity of the biocomposites was determined according to the AATC100-2004 method. The bactericidal activities of the biocomposites produced were compared with those of the gram-positive bacteria Staphylococcus aureus ATCC 25923 and the gram-negative bacteria Escherichia coli ATCC 25922. Biocomposite T010 (0.10% extract) showed strong antibacterial activity, reducing E. coli by 84.6% and S. aureus by 97.54%. The results of this study show that the T. sipyleus extract can be used as an effective antibacterial agent at appropriate concentrations and that the BC biocomposite produced by ex situ modification has excellent antibacterial properties.

Keywords

• Bacterial Cellulose
• Ex situ Modification
• Thymus sipyleus Boiss.
• Sustainable Biomaterials
• Natural Antibacterial Agents

THYMUS SIPYLEUS BOISS. EKSTRAKTI BAZLI BAKTERIYEL SELÜLOZ BIYOKOMPOZITLERININ ÜRETIMI VE KARAKTERIZASYONU

Abstract

Bu çalışmanın amacı Kombucha kültür ortamında ex situ modifikasyon ile üretilen bakteriyel selülozun (BC) antibakteriyel özelliklerini iyileştirmektir. Thymus sipyleus Boiss. bitkisinden elde edilen biyoaktif metanol özütü, BC'ye ex situ modifikasyonla eklenerek biyoaktif ve uygun maliyetli bir biyokompozit elde edilmiştir. SEM ve FTIR analizleri, nanofiberleri, gözenekli yapıyı ve özütün BC nanofiberleri ile kimyasal bağlanmasını doğruladı ve biyokompozitlerin başarıyla üretildiği kanıtlanmıştır. Biyokompozitlerde su tutma ve şişme davranışında önemli bir azalma gözlenmiştir. Biyokompozitlerin antibakteriyel aktivitesi AATC100-2004 yöntemine göre belirlenmiştir. Üretilen biyokompozitlerin bakterisidal aktiviteleri, gram pozitif bakteri Staphylococcus aureus ATCC 25923 ve gram negatif bakteri Escherichia coli ATCC 25922 ile karşılaştırılmıştır. Biyokompozit T010 (%0,10 ekstrakt) güçlü antibakteriyel aktivite göstererek E. coli'yi %84,6, S. aureus'u ise %97,54 oranında azaltmıştır. Bu çalışmanın sonuçları, T. sipyleus ekstraktının uygun konsantrasyonlarda etkili bir antibakteriyel ajan olarak kullanılabileceğini ve ex situ modifikasyonla üretilen BC biyokompozitinin mükemmel antibakteriyel özelliklere sahip olduğunu göstermektedir.

Keywords

• Bakteriyel Selüloz
• Ex situ Modifikasyon
• Thymus sipyleus Boiss.
• Sürdürülebilir Biyomalzemeler
• Doğal Antibakteriyel Ajanlar

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