CONSOLIDA THIRKEANA EKSTRESİNİN GAZ KROMATOGRAFİSİ-KÜTLE SPEKTROMETRESİ (GC-MS) ANALİZİ

Abstract

Amaç: Consolida thirkeana (Boiss.) Bornm. (Ranunculaceae) Türkiye için endemik olarak kabul edilir ve soluk leylak çiçekleri, sapsız foliküller ve linear lasiniat yaprakları ile tanımlanır. Consolida cinsi genellikle morfolojik olarak Delphinium ile karıştırılabilir, bu bakımdan Consolida cinsinin üyelerinin kemotaksonomik olarak araştırılmasına ihtiyaç duyulur. Bu çalışmada, C. thirkeana'nın fitokimyasal içeriği GC/MS analizi ile aydınlatılmıştır.

Gereç ve Yöntem: Bitki materyali Ayaş'tan (Ankara/Türkiye) toplanmıştır. Bir herbaryum örneği Ankara Üniversitesi Eczacılık Fakültesi Herbaryumu'na kaydedilmiştir. Bitkinin herbası bir öğütücüde toz haline getirildi. Toz haline getirilmiş bitki parçaları metanol kullanılarak maserasyon yöntemi ile ekstre edildi. Ekstrelerin GC-MS analizi, bir Agilent 5973N dört kutuplu kütle spektrometresi (Agilent, ABD) ile donatılmış bir Agilent 6890 gaz kromatografı kullanılarak yapıldı. Bileşiklerin belirlenmesi ve tanımlanması için Mass Hunter yazılımı (Qualitative Analysis B.07.00) ve NIST Mass Spectral Library (2014) kullanıldı.

Sonuç ve Tartışma: Bu çalışmanın analiz sonuçları, C. thirkeana metanol ekstresinde 15 bileşiğin varlığını göstermiştir. Ekstre %0.65 Glikozit, %0.68 Yağ asidi, %0.91 Açilgliserol, %2.41 Karboksilik asit, %38.5 Şeker, %56.86 Şeker alkolü içerir.

Keywords

• Consolida thirkeana
• endemik
• fitokimya
• GC/MS
• herba

GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) ANALYSIS OF CONSOLIDA THIRKEANA EXTRACT

Abstract

Objective: Consolida thirkeana (Boiss.) Bornm. (Ranunculaceae) is considered endemic to Turkey and identified by pale lilac flowers, sessile follicles, and laciniae linear leaves. The genus Consolida can often be confused morphologically with Delphinium, in this respect, members of the genus Consolida need chemotaxonomic interest. In this study, the phytochemical content of C. thirkeana was clarified by GC/MS analysis.

Material and Method: The plant material was collected from Ayaş (Ankara/Turkey). A voucher specimen was deposited in the Ankara University Faculty of Pharmacy Herbarium. The aerial part of the plant was powdered in a grinder. The powdered plant parts were macerated with methanol. The GC-MS analysis of extracts was performed using an Agilent 6890 gas chromatograph equipped with an Agilent 5973N quadrupole mass spectrometer (Agilent, USA). Mass Hunter software (Qualitative Analysis B.07.00) and the NIST Mass Spectral Library (2014) were used for determining and identifying compounds.

Result and Discussion: The analysis results of this study showed the presence of 15 compounds in C. thirkeana methanol extract. The extract contains 0.65% Glycoside, 0.68% Fatty acid, 0.91% Acylglycerol, 2.41% Carboxylic acid, 38.5% Sugar, 56.86% Sugar alcohol.

Keywords

• Consolida thirkeana
• endemic
• GC/MS
• herba
• phytochemistry

Thanks

The authors thank Dr. M. Mesud HÜRKUL for the collection of plant materials.

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