CONSOLIDA THIRKEANA EKSTRESİNİN GAZ KROMATOGRAFİSİ-KÜTLE SPEKTROMETRESİ (GC-MS) ANALİZİ

Öz

Amaç: Consolida thirkeana (Boiss.) Bornm. (Ranunculaceae) Türkiye için endemik olarak kabul edilir ve soluk leylak çiçekleri, sapsız foliküller ve linear lasiniat yaprakları ile tanımlanır. Consolida cinsi genellikle morfolojik olarak Delphinium ile karıştırılabilir, bu bakımdan Consolida cinsinin üyelerinin kemotaksonomik olarak araştırılmasına ihtiyaç duyulur. Bu çalışmada, C. thirkeana'nın fitokimyasal içeriği GC/MS analizi ile aydınlatılmıştır.

Gereç ve Yöntem: Bitki materyali Ayaş'tan (Ankara/Türkiye) toplanmıştır. Bir herbaryum örneği Ankara Üniversitesi Eczacılık Fakültesi Herbaryumu'na kaydedilmiştir. Bitkinin herbası bir öğütücüde toz haline getirildi. Toz haline getirilmiş bitki parçaları metanol kullanılarak maserasyon yöntemi ile ekstre edildi. Ekstrelerin GC-MS analizi, bir Agilent 5973N dört kutuplu kütle spektrometresi (Agilent, ABD) ile donatılmış bir Agilent 6890 gaz kromatografı kullanılarak yapıldı. Bileşiklerin belirlenmesi ve tanımlanması için Mass Hunter yazılımı (Qualitative Analysis B.07.00) ve NIST Mass Spectral Library (2014) kullanıldı.

Sonuç ve Tartışma: Bu çalışmanın analiz sonuçları, C. thirkeana metanol ekstresinde 15 bileşiğin varlığını göstermiştir. Ekstre %0.65 Glikozit, %0.68 Yağ asidi, %0.91 Açilgliserol, %2.41 Karboksilik asit, %38.5 Şeker, %56.86 Şeker alkolü içerir.

Anahtar Kelimeler

• Consolida thirkeana
• endemik
• fitokimya
• GC/MS
• herba

GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) ANALYSIS OF CONSOLIDA THIRKEANA EXTRACT

Öz

Objective: Consolida thirkeana (Boiss.) Bornm. (Ranunculaceae) is considered endemic to Turkey and identified by pale lilac flowers, sessile follicles, and laciniae linear leaves. The genus Consolida can often be confused morphologically with Delphinium, in this respect, members of the genus Consolida need chemotaxonomic interest. In this study, the phytochemical content of C. thirkeana was clarified by GC/MS analysis.

Material and Method: The plant material was collected from Ayaş (Ankara/Turkey). A voucher specimen was deposited in the Ankara University Faculty of Pharmacy Herbarium. The aerial part of the plant was powdered in a grinder. The powdered plant parts were macerated with methanol. The GC-MS analysis of extracts was performed using an Agilent 6890 gas chromatograph equipped with an Agilent 5973N quadrupole mass spectrometer (Agilent, USA). Mass Hunter software (Qualitative Analysis B.07.00) and the NIST Mass Spectral Library (2014) were used for determining and identifying compounds.

Result and Discussion: The analysis results of this study showed the presence of 15 compounds in C. thirkeana methanol extract. The extract contains 0.65% Glycoside, 0.68% Fatty acid, 0.91% Acylglycerol, 2.41% Carboxylic acid, 38.5% Sugar, 56.86% Sugar alcohol.

Anahtar Kelimeler

• Consolida thirkeana
• endemic
• GC/MS
• herba
• phytochemistry

Kaynakça

1. Brummitt, R.K. (1992). Vascular plant families and genera. Richmond, UK: Royal Botanic Gardens, Kew.
2. Heywood, V.H., Brummitt, R.K., Culham, A., Seberg, O. (2007). Flowering plant families of the world. Ontario: Firefly Books.
3. Davis, P.H. (1965). Flora of Turkey and the East Aegean Islands, (Vol. 1, pp.119-134). Edinburgh, UK: Edinburgh University Press.
4. Hürkul, M.M. (2021). Leaf, stem and root anatomy of Consolida thirkeana (Boiss.) Bornm. (Ranunculaceae). Journal of research in pharmacy, 25(4), 415-419. [CrossRef]
5. Gümüşok, S., Hürkul, M.M. (2021). Anatomy of Consolida orientalis (Gay) Schröd. (Ranunculaceae): Root, stem and leaf. İstanbul Journal of Pharmacy, 51(3), 3-4. [CrossRef]
6. POWO. (2022). Plants of the World Online. Facilitated by the Royal Botanic Gardens, Kew. http://www.plantsoftheworldonline.org Accessed: 10.04.2022.
7. Evans, W. (2002). Trease and Evans pharmacognosy (15th ed.). WB Saunders.
8. Metcalfe, C.R., Chalk, L. (1965). Anatomy of dicotyledones. (Vol. 2). Oxford, UK: Clarendon Press.

9. Hao, D.C. (2018). Ranunculales medicinal plants: Biodiversity, chemodiversity and pharmacotherapy. London, UK: Academic Press.
10. Yin, T., Cai, L., Ding, Z. (2020). A systematic review on the chemical constituents of the genus Consolida (Ranunculaceae) and their biological activities. RSC Advances, 10(58), 35072-35089. [CrossRef]
11. Güner, A., Aslan, S., Ekim, T., Vural, M., Babaç, M. T. (2012). Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyigit Botanik Bahçesi Yayınları, Flora Dizisi I.
12. Kıymacı, M.E., Tok, K.C., Hürkul, M.M. (2022). A study on phytochemical analysis and antibacterial activity of Quercus macranthera subsp. syspirensis (K. Koch) Menitsky branch and leaf extracts. Journal of Faculty of Pharmacy of Ankara University, 46(1), 160-169. [CrossRef]
13. Blau, K., Halket, J.M. (1993). Handbook of derivatives for chromatography. Wiley.
14. Awuchi, C.G., Echeta, K.C. (2019). Current developments in sugar alcohols: Chemistry, nutrition, and health concerns of sorbitol, xylitol, glycerol, arabitol, inositol, maltitol, and lactitol. International Journal of Advanced Academic Research, 5, 1-33.
15. Saraiva, A., Carrascosa, C., Raheem, D., Ramos, F., Raposo, A. (2020). Natural sweeteners: The relevance of food naturalness for consumers, food security aspects, sustainability and health impacts. International Journal of Environmental Research and Public Health, 17(17), 6285. [CrossRef]
16. Kroger, M., Meister, K., Kava, R. (2006). Low‐calorie sweeteners and other sugar substitutes: a review of the safety issues. Comprehensive reviews in food science and food safety, 5(2), 35-47. [CrossRef]
17. Ruiz-Ojeda, F.J., Plaza-Díaz, J., Sáez-Lara, M.J., Gil, A. (2019). Effects of sweeteners on the gut microbiota: a review of experimental studies and clinical trials. Advances in Nutrition, 10, 31-48. [CrossRef]
18. Mericli, A.H., Yazici, S., Eroglu-Ozkan, E., Sen, B., Kurtoglu, S., Ozcelik, H., Mericli, F. (2012). Norditerpenoid alkaloids from Consolida thirkeana and Consolida sulphurea. Chemistry of Natural Compounds, 48(3), 525-526.