TÜRKİYE’DE YETİŞEN BAZI CENTAURIUM HILL TAKSONLARININ ANTİMİKROBİYAL AKTİVİTESİ

Abstract

Amaç: Bu çalışmada, Türkiye’de yetişen beş Centaurium türünün (Centaurium erythraea subsp. rhodense, C. erythraea subsp. turcicum, C. maritimum, C. spicatum ve C. tenuiflorum subsp. acutiflorum) toprak üstü kısımlarından elde edilen etil asetat ve metanol ekstrelerinin seçilen Gram pozitif ve Gram negatif bakteriler ve Candida albicans mayasına karşı in vitro antimikrobiyal etkinliğinin test edilmesi amaçlanmıştır.

Gereç ve Yöntem: Ege (Muğla, Denizli, İzmir) ve Akdeniz (Antalya) bölgelerinden toplanan bitki örneklerinin toprak üstü kısımları kullanılarak elde edilen metanol ve etil asetat ekstrelerinin, Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 13883, Pseudomonas aeruginosa ATCC 27853 bakterileri ve Candida albicans ATCC 10231 mayasına karşı in vitro antimiktobiyal aktivitesi sıvı mikrodilüsyon yöntemi kullanılarak araştırılmıştır.

Sonuç ve Tartışma: Üzerinde çalışılan Centaurium türlerinin metanol ekstrelerinin S. aureus, E. faecalis, E. coli, K. pneumoniae, P. aeruginosa bakterileri ve C. albicans mayasına karşı orta düzeyde antimikrobiyal aktivite gösterdiği, etil asetat ile hazırlanan ekstrelerin ise etkinliğinin olmadığı tespit edilmiştir

Keywords

• Antimikrobiyal aktivite
• Centaurium erythraea subsp. rhodense
• C. erythraea subsp. turcicum
• C. maritimum
• C. spicatum
• C. tenuiflorum subsp. acutiflorum

ANTIMICROBIAL ACTIVITY OF SOME CENTAURIUM HILL TAXA GROWING IN TURKEY

Abstract

Objective: In this study, in vitro antimicrobial activity of ethyl acetate and methanol extracts of the aerial parts of five Centaurium species (Centaurium erythraea subsp. rhodense, C. erythraea subsp. turcicum, C. maritimum, C. spicatum, and C. tenuiflorum subsp. acutiflorum), which are naturally grown in Turkey were intended to be tested against selected Gram positive and Gram negative bacteria; and a yeast Candida albicans by using broth microdilution method.

Material and Method: Methanol and ethyl acetate extracts of aerial parts were obtained from each of the Centaurium plants collected from Ege (Muğla, Denizli, İzmir) and Akdeniz (Antalya) Regions in Turkey tested on S. aureus ATCC 29213, E. faecalis ATCC 29212, E. coli ATCC 25922, K. pneumoniae ATCC 13883, P. aeruginosa ATCC 27853 as bacteria and C. albicans ATCC10231 as yeast by using broth microdilution method. 

Result and Discussion: The methanol extracts of all Centaurium species have indicated moderate antimicrobial activities against S. aureus, E. faecalis, E. coli, K. pneumoniae, P. aeruginosa and C. albicans, but ethyl acetate extract have no activity.

Keywords

• Antimicrobial activity
• Centaurium erythraea subsp. rhodense
• C. erythraea subsp. turcicum
• C. maritimum
• C. spicatum
• C. tenuiflorum subsp. acutiflorum

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