TÜRKİYE’DE YETİŞEN BAZI CENTAURIUM HILL TAKSONLARININ ANTİMİKROBİYAL AKTİVİTESİ

Yıl 2020, , 415 - 423, 30.09.2020

Gülderen Yılmaz , Duygu Şimşek Gülnur Ekşi Nurten Altanlar Mehmet Çiçek A. Emre Yaprak

https://doi.org/10.33483/jfpau.756312

Cited By: 1

Öz

Amaç: Bu çalışmada, Türkiye’de yetişen beş Centaurium türünün (Centaurium erythraea subsp. rhodense, C. erythraea subsp. turcicum, C. maritimum, C. spicatum ve C. tenuiflorum subsp. acutiflorum) toprak üstü kısımlarından elde edilen etil asetat ve metanol ekstrelerinin seçilen Gram pozitif ve Gram negatif bakteriler ve Candida albicans mayasına karşı in vitro antimikrobiyal etkinliğinin test edilmesi amaçlanmıştır.

Gereç ve Yöntem: Ege (Muğla, Denizli, İzmir) ve Akdeniz (Antalya) bölgelerinden toplanan bitki örneklerinin toprak üstü kısımları kullanılarak elde edilen metanol ve etil asetat ekstrelerinin, Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 13883, Pseudomonas aeruginosa ATCC 27853 bakterileri ve Candida albicans ATCC 10231 mayasına karşı in vitro antimiktobiyal aktivitesi sıvı mikrodilüsyon yöntemi kullanılarak araştırılmıştır.

Sonuç ve Tartışma: Üzerinde çalışılan Centaurium türlerinin metanol ekstrelerinin S. aureus, E. faecalis, E. coli, K. pneumoniae, P. aeruginosa bakterileri ve C. albicans mayasına karşı orta düzeyde antimikrobiyal aktivite gösterdiği, etil asetat ile hazırlanan ekstrelerin ise etkinliğinin olmadığı tespit edilmiştir

Anahtar Kelimeler

Antimikrobiyal aktivite, Centaurium erythraea subsp. rhodense, C. erythraea subsp. turcicum, C. maritimum, C. spicatum, C. tenuiflorum subsp. acutiflorum

ANTIMICROBIAL ACTIVITY OF SOME CENTAURIUM HILL TAXA GROWING IN TURKEY

Öz

Objective: In this study, in vitro antimicrobial activity of ethyl acetate and methanol extracts of the aerial parts of five Centaurium species (Centaurium erythraea subsp. rhodense, C. erythraea subsp. turcicum, C. maritimum, C. spicatum, and C. tenuiflorum subsp. acutiflorum), which are naturally grown in Turkey were intended to be tested against selected Gram positive and Gram negative bacteria; and a yeast Candida albicans by using broth microdilution method.

Material and Method: Methanol and ethyl acetate extracts of aerial parts were obtained from each of the Centaurium plants collected from Ege (Muğla, Denizli, İzmir) and Akdeniz (Antalya) Regions in Turkey tested on S. aureus ATCC 29213, E. faecalis ATCC 29212, E. coli ATCC 25922, K. pneumoniae ATCC 13883, P. aeruginosa ATCC 27853 as bacteria and C. albicans ATCC10231 as yeast by using broth microdilution method. 

Result and Discussion: The methanol extracts of all Centaurium species have indicated moderate antimicrobial activities against S. aureus, E. faecalis, E. coli, K. pneumoniae, P. aeruginosa and C. albicans, but ethyl acetate extract have no activity.

Anahtar Kelimeler

Antimicrobial activity, Centaurium erythraea subsp. rhodense, C. erythraea subsp. turcicum, C. maritimum, C. spicatum, C. tenuiflorum subsp. acutiflorum

Kaynakça

• 1. Carmona F., Pereira A.M.S. (2013). Herbal medicines: old and new concepts, truths and misunderstandings, Braz J Pharmacogn, 23(2): 379-385.
• 2. Shakya A.K. (2016). Medicinal plants: Future source of new drugs, International Journal of Herbal Medicine, 4(4): 59-64.
• 3. Srivastava J., Lambert J., Vietmeyer N. (1996). Medicinal plants: An expanding role in development. World Bank Technical Paper Number 320.
• 4. Mahesh B., Satish S. (2008). Antimicrobial Activity of Some Important Medicinal Plant Against Plant and Human Pathogens. World J. Agric. Sci, 4 (S): 839-843.
• 5. Compean K.L., Ynalvez R.A. (2014), Antimicrobial Activity of Plant Secondary Metabolites: A Review. Research Journal of Medicinal Plants, 8: 204-213.
• 6. Gokhale M., Wadhwani M. (2015). Antimicrobial Activity of Secondary Metabolites from Plants - A Review. International Journal of Pharmacognosy, 2(2): 60-65.
• 7. Davis P.H. (1978). Flora of Turkey and the East Aegean Islands, Edinburgh Uninersity Press, vol. 6. Edinburgh,178-180.
• 8. Güner A. (2012). Türkiye Bitkileri Listesi (Damarlı Bitkiler) Nezhat Gökyiğit Botanik Bahçesi Yayınları Flora Dizisi, İstanbul, 512.
• 9. Baytop T. (1999). Türkiye’de Bitkiler ile Tedavi (Geçmişte ve Bugün), İlaveli 2. Baskı, Nobel Tıp Kitabevleri, İstanbul: 243.
• 10. Newall C.A., Anderson L.A. (1994). Herbal Medicines-Guide for Healthcare Professionals. London: Pharmaceutical Press, 6. 11. Iordanov D. (1970). Fitoterapiíà: Lechenie Lekarstvennymi Travami, Meditsina iFizkul'tura, Sofia,
• 12. Tucakov J. (1990). Healing with plants. Beograd: Rad, 24-37.
• 13. Zlatković B.K., Bogosavljević S.S., Radivojević A.R., Pavlović M.A. (2014). Traditional use of the native medicinal plant resource of Mt. Rtanj (Eastern Serbia): Ethnobotanical evaluation and comparison. J Ethnopharmacol, 151(1), 704-713.
• 14. Pieroni A., Quave C.L., Santoro R.F. (2004), Folk Pharmaceutical Knowledge in the Territory of the Dolomiti Lucane, Inland Southern Italy, 95, 373–384.
• 15. 15- Bojan K. Zlatkovi, Bogosavljevi Stefan S., Radivojevi Aleksandar R., Pavlovi Mila A. (2014). Traditional use of the native medicinal plant resource of Mt. Rtanj (Eastern Serbia): Ethnobotanical evaluation and comparison, Journal of Ethnopharmacology, 151, 704-713.
• 16. Hansel R., Keller K., Rimpler H., Schneider G. (Hrsg.). (1994). Hagers Handbuch der Pharmazeutischen Praxis, 5. Aufl., Bde 4-6 (Drogen), Springer Verlag Berlin, Heidelberg, New York.
• 17. Berkan T., Üstünes L., Lermioglu F., Özer A. (1991). Antiinflammatory, analgesic and antipyretic effects of an aqueous extract of Erythraea centaurium. Planta Med. 57, 34–3.
• 18. Dweck A.C. (1997). Research Director, Peter Black Medicare Ltd., Trowbridge, Wilts., Ethnobotanıcal Use Of Plants Part 4, The Amerıcan Contınent .
• 19. Bisset N.G., Max Wichtl’s (1994).Herbal Drugs and Phytopharmaceuticals, A handbook for practice on a scientific basis, Scientific Publisher, Stuttgar, 134-136.
• 20. Walther H.J. (2004) Tausendguldenkraut-Heilpflanze des Jahres, Pharmaceutische Zeitung, 15(30),
• 21. Polat R., Satı F., (2012). An ethnobotanical survey of medicinal plants in Edremit Gulf (Balıkesir– Turkey) Journal of Ethnopharmacology, 139, 629-641,
• 22. Tuzlacı E. (2006). Şifa Niyetine, Türkiye’nin Bitkisel Halk İlaçları, Alfa Yayınları, İstanbul, 68.
• 23. Orhan I.E., Senol F.S., Haznedaroglu MZ, Koyu H, Erdem SA, Yılmaz G., Çicek M., Yaprak A.E., Arı E., Küçükboyacı N., Toker G. (2017). Neurobiological evaluation of thirty-one medicinal plant extracts using microtiter enzyme assays. Clinical Phytoscience, 2(1), 9.
• 24. Yılmaz, G. (2017). Centaurium erythraea, In.Demirezer,L.Ö.,(Eds). FFD MonograflarıBitkiler ve Etkileri, Akademisyen Kitabevi, Ankara, 235-242.
• 25. Kırbağ S., Zengin F., Kurşat M. (2009). Antimicrobial activities of extracts of some plants. Pakistan Journal of Botany, 41(4): 2067-2070.
• 26. Šiler B., Zˇivkovic S., Banjanac T., Cvetkovic´ J., ´ Zˇivkovic´ J.N., C´ Iric A., Sokovic´ M., Mišic D. (2014). Centauries asunderestimated food additives: Antioxidant and antimicrobial potential. Food Chemistry, 147, 367-376.
• 27. Bouyahya A., Belmehdi O., El Jemli M., Marmouzi I., Bourais I., Abrini J., Faouzi My El A., Dakka N., Bakri Y. (2019). Chemical variability of Centaurium erythraea essential oils at three developmental stages and investigation of their in vitro antioxidant, antidiabetic, dermatoprotective and antibacterial activities. Industrial Crops & Products, 132, 111–117.
• 28. NCCLS. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; (2002). Approved Standard—Second Edition. NCCLS document M27-A2 [ISBN 1-56238-469-4]. NCCLS, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087-1898 USA,
• 29. CLSI. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically (2012). Approved Standard—Ninth Edition. CLSI document M07-A9. Wayne, PA: Clinical and Laboratory Standards Institute.