BİTLİS YÖRESİNDEN ALINAN BAZI BALLARIN BOTANİK VE BİYOKİMYASAL PROFİLİ

Year 2020, Volume: 44 Issue: 3, 494 - 505, 30.09.2020

İlginç Kizilpinar Temizer , Aytaç Güder , Birol Başer

https://doi.org/10.33483/jfpau.767197

Abstract

Amaç: Bu makalede 2017 yılında Bitlis bölgesinden rastgele seçilen 4 bal örneğinin polen kompozisyonunu ve antioksidan aktivitesini değerlendirmeyi amaçladık.

Gereç ve Yöntem: Melisopalinolojik analizde asetolizsiz standart protokol kullanılmıştır. Numunelerin antioksidan aktivitesi, 1,1-difenil-2-pikrilhidrazil serbest radikal giderme aktivitesi (DPPH), hidrojen peroksit giderme aktivitesi (HPSA), demirli iyon şelat aktiviteleri (FICA), 2,2'-azino-bis-3-etilbenztiyazolin-6-sülfonik asit radikal katyon temizleme deneyleri (ABTS) ve ferrik indirgeyici antioksidan güç deneyleri (FRAP) gibi birkaç in vitro yöntem kullanılarak değerlendirilmiştir.

Sonuç ve Tartışma: 4 balın polen kompozisyonu değerlendirildiğinde balların multifloral olduğu görülmüştür. Elde edilen sonuçlar, antioksidan aktivitesinin baldan bala belirgin bir şekilde değiştiğini göstermiştir. ABTS ve FICA örnekleri birbirlerinden biraz farklı, DPPH, HPSA ve FRAP sırasıyla 54.45 - 387.60 ug / mL, 212.11 - 246.64 ug / mL ve % 47.20 - 78.23 arasında bulunmuştur. Ek olarak, toplam fenol ve toplam flavonoid içerikleri belirlenmiştir. Örnekler kıyaslandığında 3, en yüksek fenolik ve flavonoid içeriği (626.48 mg GAE / 100 g ve 4.4 mg CAE / 100 g-etanol) göstermiştir. Hemen hemen tüm numunelerin iyi bir antioksidan kaynağı olduğu tespit edilmiştir.

Keywords

Antioksidan, etanol, multifloral, palinoloji

DETERMINATION OF SEVERAL BITLIS HONEYS BASED ON THEIR BOTANIC AND BIOCHEMICAL PROFILES

Abstract

Objective: In this paper, we aimed to evaluate the pollen composition and antioxidant activity of 4 randomly selected honey samples from the Bitlis region in 2017.

Material and Method: The melisopalynological analysis was used standard protocol without acetolysis. The antioxidant activity of samples were evaluated using several in vitro methods, 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity (DPPH), hydrogen peroxide scavenging activity (HPSA), Ferrous ions chelating activities (FICA), 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid radical cation scavenging assays (ABTS) and the ferric reducing antioxidant power assays (FRAP).

Result and Discussion: All the samples were determined as multifloral honey. Outcomes obtained declared that the antioxidant activity changed remarkably from honey to honey. ABTS and FICA of samples differ slightly each other’s. DPPH, HPSA, and FRAP were ranged from between 54.45 – 387.60 µg/mL, 212.11 – 246.64 µg/mL and 47.20 – 78.23 %, respectively. In addition, total phenol and total flavonoid contents were determined. Comparatively, Sample 3 showed highest levels of phenolic and flavonoid content (626.48 mg GAE/100 g and 4.4 mg CAE/100 g, respectively) in ethanol extract. Almost all the samples are well source for antioxidants.

Keywords

Antioxidant, ethanol, multiflora, palynology

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