PLACENTA-ASSOCIATED PLASMA PROTEIN AND FREE HUMAN CHORIONIC GONADOTROPIN LEVELS IN DAY 3 VERSUS DAY 5 TRANSFER

Abstract

Amaç: Bu çalışma blastokist evresi embriyo transferi ile klivaj evresi embriyo transferinde plasenta ilişkili plasma protein A (PAPP-A) ile serbest insan koryonik gonadotropin B (B-hCG) arasında farklılık olup olmadığını araştırmak amacıyla yürütülmüştür. Gereç ve yöntemler: Bu retrospektif çalışmaya toplam 449 kadın dahil edilerek iki çalışma grubu oluşturuldu. Grup 1: İntrasitoplazmik sperm enjeksiyonu yapılarak klivaj evresi (2. veya 3. gün) taze embriyo transferi sonrası gebe kalan ve 11-14. gebelik haftasında birinci trimester fetal anöploidi biyokimyasal markerlarına bakılan 275 kadından oluşmaktadır. Grup 2: İntrasitoplazmik sperm enjeksiyonu yapılarak blastokist evresi (5. gün) taze embriyo transferi sonrası gebe kalan ve 11-14. gebelik haftasında birinci trimester fetal anöploidi biyokimyasal markerlarına bakılan 174 kadın çalışma kapsamına alınmıştır. Kadınların demografik özellikleri, infertilite sebebi, infertilite süresi, protokol rejimi, total gonadotropin dozu, follikül ve toplanılan oosit sayısı, endometrial kalınlık, funsus embriyo arası mesafe kaydedildi ve PAPP-A ile serbest B-hCG düzeyleri ölçüldü. Bulgular: PAPP-A ve serbest B-hCG düzeyleri, protokol rejimi, endometrial kalınlık ve fundus-embryo arası mesafe de istatistiksel olarak anlamlı fark bulunamamıştır. Azalmış over reservi ve total gonadotropin dozu grup 1 de (p < 0.05) ve follikül sayısı, toplanan oosit sayısı ve MII sayısı anlamlı olarak grup 2 de yüksek bulunmuştur (p < 0.05). Sonuç: 3. gün ve 5. gün taze embriyo transferi arasında PAPP-A ve serbest B-hCG düzeylerinde fark gözlenmemiştir.

Keywords

• PAPP-A
• serbest B- hCG
• 3. gün ve 5. gün taze embriyo transferi

PLACENTA-ASSOCIATED PLASMA PROTEIN AND FREE HUMAN CHORIONIC GONADOTROPIN LEVELS IN DAY 3 VERSUS DAY 5 TRANSFER

Abstract

Aim: This study was conducted to investigate placenta-associated plasma protein (PAPP-A) and free human chorionic gonadotropin (B-hCG) levels after blastocyst transfer versus cleavage-stage embryo transfer. Materials and method: A total of 449 women were included in this rerospective sudy. The study consisted of two groups: Group 1: pregnant women conceived by intracytoplasmic sperm injection (ICSI) procedures after fresh embryo transfer at the cleavage stage (day 2 or day 3) and had first trimester fetal aneuploidy biochemical markers performed at 11-14th gestational week (n: 275). Group 2: pregnant women conceived by ICSI procedures after fresh embryo transfer at the blastocyst stage (day 5) and had first trimester fetal aneuploidy biochemical markers performed at 11-14th gestational week (n:174). Demographic characteristics, causes of infertility, duration of infertility, stimulation protocol regimens, total gonadotropin doses, number of follicles and oocytes retrieval, endometrial thickness, fundus –embryo distance were recorded and placenta-associated plasma protein (PAPP-A) and free human chorionic gonadotropin (B-hCG) levels were measured. Results: We found no significant differences in PAPP-A and free B-hCG levels, stimulation protocols regimens and endometrial thickness and fundus-embryo distance. Diminished ovarian reserve and total gonadotropin doses were significiantly higher in group 1 (p < 0.05). Number of follicles, number of oocytes retrieval and MII oocytes were significiantly higher in group 2 (p < 0.05). Conclusion: No difference was observed in PAPP-A and free B-hCG levels between the 3rd and the 5th day fresh transfer.

Keywords

• PAPP-A
• free B- hCG
• 3rd -5th day fresh embryo transfer

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