Gastrik Kanser Hücre Hattına Uygulanan Kafeik Asit Fenetil Ester’in Biyomarkerleri Üzerine Etkileri

Year 2020, , 24 - 31, 15.03.2020

Tuba İmren Funda Kosova Feyzan Özdal Kurt Ercüment Ölmez İbrahim Tuğlu Zeki Arı Zeynep Kasar

https://doi.org/10.35333/JOHSE.2020.192

Abstract

Mide kanseri yüksek insidansa sahiptir ve hem erkeklerde
hem de kadınlarda mortalitenin önde gelen nedenlerinden biridir.
Anjiyogenez, büyümeyi desteklemek için oksijen ve besin
maddelerinin dokulara taşındığı mevcut kan damarlarından yeni
kılcal damarların oluşumudur ve onkogenlerin neden olduğu
kanserin invazyonunda ve metastazında önemli bir rol oynar. Bu
işlem doku onarımı ve normal büyüme için yararlı olsa da, kanser
dokularının büyümesini ve hematojen yol yoluyla metastazını
kolaylaştırır ve bu şekilde metastazı ve invasyonu artırır. Bu
çalışmada mide kanseri hücre kültürüne uygulanan CAPE’nin
VEGF, MMP, TSP-1 ve Endostatin gibi anjiyojenik ve antianjiyojenik
belirteçler arasındaki ilişkiyi araştırmayı amaçladık.
CAPE uygulamasını takiben sitotoksisite, MTT testi ile ölçüldü.
Hücrelerin anjiyojenik davranışı immünohistokimyasal olarak
incelendi ve VEGF, MMP ve Endostatin ile TSP-1 seviyesi LC
PCR testi ile ölçüldü. MTT testi, 0,5 μg/mL’lik bir konsantrasyonda
uygulanan CAPE’nin, bir mide kanseri hücre hattı üzerinde
sitotoksik bir etki yaptığını gösterdi. LC PCR ile gösterilen
gen ekspresyonu, VEGF, MMP ve TSP seviyelerinin azalırken
endostatin seviyesinin arttığını gösterdi. MMP, VEGF, TSP ve
endostatindeki değişiklikler, CAPE’nin mide kanseri üzerinde
önemli bir terapötik etkiye sahip olduğunu düşündürmektedir. Bu
bulgular ışığında, doğal ve ucuz bir madde olan CAPE, kanser
hastalarının tedavisinde etkili olabilir. Daha ileri çalışmaların
yapılması gerekir.

Keywords

Anjiogenezis, Hücre Kültürü, VEGF, MMP, CAPE, Endostatin, TSP-1

The Effects on Biomarkers of Caffeic Acid Phenethyl Ester Applied to a Gastric Cancer Cell Line

Abstract

Gastric cancer has a high incidence and is one of the leading
causes of mortality both in males and females. Angiogenesis
is the formation of new capillaries from existing blood vessels
through which oxygen and nutrients are transported to the tissues
to promote growth, and it plays a pivotal role in the invasion
and metastasis of cancer caused by oncogenes. Even though
this process is useful in tissue repair and normal growth, it also
facilitates the growth of cancer tissues and their metastasis via
the hematogenous route, and in this way, it speeds up metastasis
and invasion. In the present study, we aimed to investigate
the relationship between angiogenesis and markers such as
VEGF, MMP, TSP-1 and Endostatin in a CAPE-administered
gastric cancer cell in culture. Following CAPE administration,
cytotoxicity was measured by MTT test. Angiogenic behavior of
cells was examined immunohistochemically by VEGF, MMP and
Endostatin, and TSP – 1 level was measured by RT-PCR test. MTT
test showed that CAPE administered at a concentration of 0.5 μg/
mL exerted a cytotoxic effect on a gastric cancer cell line. Gene
expression depicted by RT-PCR showed that VEGF, MMP and TSP
levels were decreased , while the endostatin level was increased.
Changes in MMP, VEGF, TSP and endostatin suggest that CAPE
has a significant therapeutic effect on gastric cancer. In the light
of these findings, CAPE, which is a natural and inexpensive
substance, could be effective in the treatment of cancer patients
and advanced studies need to be carried out.

Keywords

Angiogenesis, Cell culture, VEGF, MMP, CAPE, Endostatin, TSP-1

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