COVID-19 Şüpheli Hastalarda Total Antikor Testlerinin Bilgisayarlı Tomografi ve Polimerize Zincir Reaksiyon Test Sonuçları ile Değerlendirmesi

Year 2023, , 1 - 6, 29.03.2023

Hacer İşler , Melek Bilgin Eşe Başbulut , Selim Görgün , Cemanur Aygün , Alper Tahmaz , Eda Köksal , Eylem Burcu Reşörlü Mirsad Yalçınkaya

https://doi.org/10.58854/jicm.1206881

Abstract

Amaç: COVID-19’un erken tanımlanması, hastalık tedavisi ve yönetiminde çok önemlidir. Kanda spesifik antikorların tespitiyle solunum yolu örneklerinden kaynaklanan yanlış negatif sonuçların önüne geçilebilir. Çalışmada COVID-19 şüphesi olan hastaların nazofaringeal sürüntülerinden alınan Hızlı Antikor Testleri, Akciğer BT taramaları ve polimeraz zincir reaksiyonu testlerinin sonuçlarını değerlendirilmesi amaçlanmıştır.

Yöntem: 15.03.2020-15.06.2020 tarihleri arasında COVID-19 şüphesi olan hastalardan ve şikayeti olan sağlık çalışanlarından alınan venöz kan örnekleri, Hodget Card Hızlı Antikor Testi (IgM + IgG) ile SBU Samsun Eğitim ve Araştırma Hastanesi mikrobiyoloji laboratuvarımızda test edildi. Çalışmada 295 kişinin hızlı antikor testi yapıldı.

Bulgular: Hastaların yaş ortalaması 52±17,9 olup %55,4’ü erkekti. %39,3’ü poliklinikte, %39’u serviste, %19’u yoğun bakımda hastasıydı. Hızlı antikor testi yapılan 295 hastanın %56,6’sında PCR testinin pozitif çıktığı, %9,8’inde negatif olduğu, %33,6’sında PCR testi çalışılmadığı belirlendi.

Sonuç: Çalışmamızda toraks BT’nin COVID-19’u tanımlamada PCR’den duyarlı olduğu gösterildi. Altın standart olan PCR testi negatif kişilerde hızlı antikor testlerinin pozitifliği , antikor testinin, CT veya PCR testi ile gözden kaçan hastalarda hasta doğrulaması için yararlı olduğunu, PCR testinin antikor testleri ile kombine kullanılmasının gerekliliğini göstermiştir.

Keywords

COVID-19, SARS CoV-2, Hızlı Antikor Testi, Toraks BT, PCR

Evaluation of Total Antibody Tests in Patients with Suspected COVID-19 by Computed Tomography and Polymerized Chain Reaction Test Results

Abstract

Objective: Early identification of COVID-19 is crucial in disease treatment and management. False negative results from respiratory tract samples can be avoided by detecting specific antibodies in the blood. The aim of the study was to evaluate the results of Rapid Antibody Tests, Lung CT scans and polymerase chain reaction tests taken from nasopharyngeal swabs of patients with suspected COVID-19.

Methods: Venous blood samples taken from patients with suspected COVID-19 and healthcare workers with complaints between 15.03.2020 and 15.06.2020 were tested in our microbiology laboratory with Hodget Card Rapid Antibody Test (IgM + IgG) at SBU Samsun Training and Research Hospital. Rapid antibody testing was performed on 295 people in the study.

Results: The mean age of the patients was 52±17.9 years and 55.4% were male. 39.3% of them were in the outpatient clinic, 39% were in the service, and 19% were in the intensive care unit. It was determined that of 295 patients who underwent rapid antibody test, the PCR test was positive in 56.6%, negative in 9.8%, and no PCR test in 33.6%.

Conclusion: In our study, it was shown that thorax CT is more sensitive than PCR in identifying COVID-19. The positivity of rapid antibody tests in people with negative PCR testing, which is the gold standard, showed that antibody testing is useful for patient confirmation in patients missed by CT or PCR testing, and that PCR testing should be used in combination with antibody tests.

Keywords

COVID-19, SARS CoV-2, Rapid Antibody Test, Thorax CT, RT-PCR

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