Mechanisms of oral tolerance to insulin in offspring of rats with experimental gestational diabetes

Year 2017, , 28 - 33, 30.06.2017

Tanya Prozorova

https://doi.org/10.5455/jicm.20170204

Abstract

Background: The
aim of our work was to find out the mRNA gene expression level of Aire, Deaf1, Foxp3, Ctla4 and IL10 in mesenteric lymph nodes in the
offspring of rats with experimental gestational diabetes and in conditions of
insulin oral tolerance formation.

Material and Methods: Using molecular genetic and immunofluorescence techniques we
investigated the mRNA gene expression level of Aire, Deaf1, Foxp3, Ctla4 and IL10 in the offspring of rats with
experimental gestational diabetes and in conditions of insulin oral tolerance
formation. To determine the level of mRNA studied gene RT-PCR was performed in
real time thermocycler CFX96 ™ Real-Time PCR Detection Systems.

Results: We
observed such violations of immunotolerance: AIRE gene repression, reduced mRNA
levels of Deaf1 and the transcription factor Foxp3. This was accompanied by inhibition of gene expression
suppressor cytokine IL-10 and negative costimulatory molecules Ctla4.

Conclusion: Oral
insulin during the first 2 weeks graded these changes, causing transcriptional
activation of genes AIRE, Deaf1, Foxp3,
Ctla4 and Il-10.

Keywords

Experimental gestational diabetes, mesenteric lymph nodes, Aire, Deaf1, Foxp3

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