Correlation between anti-dsDNA, complement components C3, C4 and Systemeic Lupus Erythematosus disease activity index

Year 2017, , 54 - 61, 30.09.2017

Therese Mary Dhason

https://doi.org/10.5455/jicm.26-20170707

Cited By: 2

Abstract

Background: To
find out the correlation between anti-ds DNA, complement components C3, C34 and
disease activity in SLE. Material and
Methods: This cross-sectional study was undertaken over a period of 6
months from January 2016 to June 2016 at a Tertiary Care Centre in South
India.  Blood samples were collected from
100 patients with active lupus, 100 patients with inactive disease and 100
healthy controls. Serum samples from all the 300 samples were tested for anti-
dsDNA by ELISA and C3, C4 by Radial Immunodiffusion. Results: High titre anti-dsDNA was detected in 48 out of the 100
patients with active disease. Mean SLE disease activity index (SLEDAI) of
patients with high titre anti-dsDNA was 23.08337 ± 3.80276 (P<0.001) Mean
SLEDAI of patients with very low C3 and C4 was 21.4314 ± 4.88776 (P<0.001)
and  21.1250 ± 4.82882 (P<0.001).  Conclusion:
Anti-dsDNA, C3, C4 levels can be adopted as markers of disease activity in SLE.
Anti-dsDNA by ELISA is a simple and cost effective method. Radial
immunodiffusion can be done for C3 and C4 assay where nephelometer is not
available as the procedure is simple.

Keywords

Systemic Lupus Erythematosus, anti-ds DNA antibody, ELISA, C3, C4, radial immuno diffusion

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