Comparison of Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry with VITEK2 Gram positive system for identification of beta hemolytic Streptococci

Year 2018, Volume: 3 Issue: 1, 1 - 7, 30.03.2018

Yeşim Beşli

https://doi.org/10.5455/jicm.29.20171123

Abstract

Background: Matrix–assisted
laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is
known as a successful tool for bacterial identification and suitable for
clinical diagnostics. The aim of our study was to evaluate diagnostic accuracy
of MALDI-TOF MS system and VITEK2 Gram Positive Identification (GP ID) system
for the identification of Beta Hemolytic Streptococci (BHS).

Materials and Methods: A total 148 BHS which were isolated from various clinical
specimens were included in the study. Isolates were identified by Bruker
Biotyper 3.4 and VITEK2 GP ID coupled to the agglutination test. Identification
results for Bruker Biotyper 3.4 and VITEK2 GP ID systems were classified in
three different categories as follows: correct identification, low level of
discrimination, no identification/misidentification. Identification results of
BHS were evaluated according to these categories.

Results: BHS
isolates were defined as Streptococcus pyogenes (n: 69), Streptococcus agalactiae (n: 59), Streptococcus dysgalactiae serogroup C (n: 5), and Streptococcus dysgalactiae serogroup G
(n: 15) according to latex agglutination assay. MALDI-TOF MS analysis of 148
BHS isolates yielded correct identification for 89.9% of S. pyogenes, 64.4% of S.
agalactiae, and 95.0% S.
dysagalactiae serogroup C and G; while VITEK2 GP ID gave correct
identification for 62.3% of S. pyogenes,
67.8% of S. agalactiae, and 65.0% of S. dysagalactiae serogroup C and G.

Conclusions:
MALDI-TOF MS-based identification provides faster and more accurate
identification of beta-hemolytic streptococcal species than VITEK2 GP ID system.

Keywords

MALDI-TOF, VITEK, Beta Hemolytic Streptococci, S. pyogenes, S. agalactiae, S. dysagalactiae

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