Investigation of liver autoantibodies in anticentromere antibody positive patients: A preliminary research

Year 2016, Volume: 1 Issue: 1, 14 - 17, 30.06.2016

Asli Gamze Sener

https://doi.org/10.5455/jicm.8.20160529

Abstract

Background: Anticentromere
antibody (ACA) is regarded to be a serological marker specific to CREST
(calcinosis, Raynaud’s phenomenon, esophageal dysmotility, sclerodactylia, and
telangiectasia) syndrome. ACA is also found in the sera of patients with
autoimmune liver disease. In the present work, anti-soluble liver antigen\liver
pancreas antigen (anti-SLA\LP), anti-liver cytosolic antigen 1 (anti-LC1),
anti-liver kidney microsomal antigen 1 (anti-LKM1), and anti-mitochondrial
antibody M2 (AMA-M2) were evaluated in the patients who had positive
anticentromere antibody.

Material and Methods: A total of 39 patients who were positive anticentromere
antibody were enrolled in this study undertaken in the Izmir Katip Celebi
University, Ataturk Training and Research Hospital, Microbiology laboratory
between January and September 2015. Positive anticentromere antibody and liver
autoantibodies were analyzed. Anticentromere antibody and liver autoantibodies
were studied by indirect immunofluorescence method (IIF) and immunoblotting
method (IB), respectively. The patients who had negative anticentromere
antibody were used as a control group.

Results: According
to the study’s results, positivity was detected in 3 of 39 patients (%7.6) in
terms of liver autoantibodies, all of which were AMA-M2. There was no
statistically significant difference between ACA and autoimmune liver
autoantibodies.

Conclusion: In
this study, we reported our preliminary experience to provide evidence for the
detection of various autoantibodies as potential diagnostic or prognostic
tests. Further studies that contain a broad range of patients may contribute to
the field.

Keywords

Anticentromere antibody, autoimmune hepatitis, immunofluorescence method

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