Year 2018, Volume , Issue , Pages 27 - 33 2018-06-05

Mechanisms of oral tolerance to insulin in offspring of rats with experimental gestational diabetes

Tanya Prozorova, et al. [1]


Background: The aim of our work was to find out the mRNA gene expression level of Aire, Deaf1, Foxp3, Ctla4 and IL10 in mesenteric lymph nodes in the offspring of rats with experimental gestational diabetes and in conditions of insulin oral tolerance formation.

Material and Methods: Using molecular genetic and immunofluorescence techniques we investigated the mRNA gene expression level of Aire, Deaf1, Foxp3, Ctla4 and IL10 in the offspring of rats with experimental gestational diabetes and in conditions of insulin oral tolerance formation. To determine the level of mRNA studied gene RT-PCR was performed in real time thermocycler CFX96 ™ Real-Time PCR Detection Systems.

Results: We observed such violations of immunotolerance: AIRE gene repression, reduced mRNA levels of Deaf1 and the transcription factor Foxp3. This was accompanied by inhibition of gene expression suppressor cytokine IL-10 and negative costimulatory molecules Ctla4.

Conclusion: Oral insulin during the first 2 weeks graded these changes, causing transcriptional activation of genes AIRE, Deaf1, Foxp3, Ctla4 and Il-10.

Experimental gestational diabetes, mesenteric lymph nodes, Aire, Deaf1, Foxp3
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Primary Language en
Subjects Medicine
Journal Section Research Articles
Authors

Author: Tanya Prozorova, et al. (Primary Author)

Dates

Publication Date : June 5, 2018

Bibtex @research article { jicm422127, journal = {Journal of Immunology and Clinical Microbiology}, issn = {}, eissn = {2528-9470}, address = {Adalet mh. 1643/18 Sk. bina no:6 smyrnapark evleri b blok daire 16, Bayraklı, İzmir}, publisher = {Erkan YULA}, year = {2018}, volume = {}, pages = {27 - 33}, doi = {10.5455/jicm.20170204}, title = {Mechanisms of oral tolerance to insulin in offspring of rats with experimental gestational diabetes}, key = {cite}, author = {Prozorova, et al., Tanya} }
APA Prozorova, et al., T . (2018). Mechanisms of oral tolerance to insulin in offspring of rats with experimental gestational diabetes. Journal of Immunology and Clinical Microbiology , () , 27-33 . DOI: 10.5455/jicm.20170204
MLA Prozorova, et al., T . "Mechanisms of oral tolerance to insulin in offspring of rats with experimental gestational diabetes". Journal of Immunology and Clinical Microbiology (2018 ): 27-33 <https://dergipark.org.tr/en/pub/jicm/issue/36924/422127>
Chicago Prozorova, et al., T . "Mechanisms of oral tolerance to insulin in offspring of rats with experimental gestational diabetes". Journal of Immunology and Clinical Microbiology (2018 ): 27-33
RIS TY - JOUR T1 - Mechanisms of oral tolerance to insulin in offspring of rats with experimental gestational diabetes AU - Tanya Prozorova, et al. Y1 - 2018 PY - 2018 N1 - doi: 10.5455/jicm.20170204 DO - 10.5455/jicm.20170204 T2 - Journal of Immunology and Clinical Microbiology JF - Journal JO - JOR SP - 27 EP - 33 VL - IS - SN - -2528-9470 M3 - doi: 10.5455/jicm.20170204 UR - https://doi.org/10.5455/jicm.20170204 Y2 - 2019 ER -
EndNote %0 Journal of Immunology and Clinical Microbiology Mechanisms of oral tolerance to insulin in offspring of rats with experimental gestational diabetes %A Tanya Prozorova, et al. %T Mechanisms of oral tolerance to insulin in offspring of rats with experimental gestational diabetes %D 2018 %J Journal of Immunology and Clinical Microbiology %P -2528-9470 %V %N %R doi: 10.5455/jicm.20170204 %U 10.5455/jicm.20170204
ISNAD Prozorova, et al., Tanya . "Mechanisms of oral tolerance to insulin in offspring of rats with experimental gestational diabetes". Journal of Immunology and Clinical Microbiology / (June 2018): 27-33 . https://doi.org/10.5455/jicm.20170204
AMA Prozorova, et al. T . Mechanisms of oral tolerance to insulin in offspring of rats with experimental gestational diabetes. J Immunol Clin Microbiol. 2018; 27-33.
Vancouver Prozorova, et al. T . Mechanisms of oral tolerance to insulin in offspring of rats with experimental gestational diabetes. Journal of Immunology and Clinical Microbiology. 2018; 33-27.