REAL-TIME (RT-PCR) DETECTION OF MONKEYPOX VİRUS

Year 2022, Volume: 7 Issue: 3, 67 - 73, 30.11.2022

Veysel Tahiroğlu , Naci Ömer Alayunt , Cihat Öztürk

Abstract

Polymerase Chain Reaction (PCR) is in vitro replication of small DNA fragments that allows for accelerated amplification of specific sequences. A sensitive technique, only DNA traces are needed for PCR to produce enough copies to be analyzed. In molecular diagnostic laboratories, RT-PCR technique is applied to find target RNAs for diagnosis of specific pathogens. With this technique, the diagnosis of Monkeypox Virus (MPXV), a double-stranded DNA virus from the zoonotic Poxviridae family, can be achieved. Poxviruses are brick-shaped and surrounded by a lipoprotein envelope with a linear double-stranded DNA genome. Besides host ribosomes for mRNA translation, poxviruses contain all essential replication, transcription, assembly and exit proteins in their genomes. The rRT-PCR method, which has high specificity and moderate sensitivity, can be a great source of hope for MPXV. We believe that when diagnosing MPXV, real-time PCR (RT-PCR) analysis and new RT-PCR studies are needed with newly developed unique rapid tests to determine whether it is sufficient to quickly distinguish similar infections.

Keywords

Monkeypox, RT-PCR, DNA, Human, Test kit

MAYMUN ÇİÇEĞİ VİRÜSÜ’NÜN REAL TİME (RT-PCR) İLE SAPTANMASI

Abstract

Polimeraz Zincir Reaksiyonu (PCR), küçük DNA fragmanlarında belirli dizilerin hızlandırılmış amplifikasyonuna izin veren in vitro replikasyonudur. Hassas bir teknik olan PCR’nin, analiz edilecek kadar kopya üretmesi için yalnızca DNA izlerine ihtiyaç vardır. Moleküler tanı laboratuvarlarında, spesifik patojenlerin teşhisi için hedef RNA’ları bulmak için RT-PCR tekniği uygulanır. Bu teknik ile zoonoz olan Poxviridae ailesinden çift sarmallı bir DNA virüsü olan maymun çiçeği virüsü (MPXV) teşhisi sağlanabilir. RT-PCR tekniği ile analiz edilecek MPXV’nin yapısı nispeten büyüktür (200-250 nanometre). Poksvirüsler tuğla şeklindedir ve lineer çift sarmallı DNA genomuna sahip bir lipoprotein zarfı ile çevrilidir. mRNA translasyonu için konakçı ribozomlarının yanı sıra, poksvirüsler genomlarında gerekli tüm replikasyon, transkripsiyon, montaj ve çıkış proteinlerini içerir. Özgüllüğü yüksek ve duyarlılığı orta düzeyde olan rRT-PCR yöntemi MPXV için büyük bir umut kaynağı olabilir. MPXV teşhisi yapılırken real-time PCR (RTPCR) analizinin ve benzer enfeksiyonları hızlı bir şekilde ayırt etmek için yeterli olup olmadığı konusunda yeni geliştirilen benzersiz hızlı testler ile yapılacak yeni analiz yöntemlerine ve yeni RTPCR çalışmalarına ihtiyaç olduğu kanaatindeyiz.

Keywords

Maymun Çiçeği, RT-PCR, DNA, İnsan, Test kiti

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