Determination of genotoxic effects in vitro of ZnOTiO2 nanoparticles on human peripheral lymphocytes

Year 2019, Volume: 14 Issue: 1, 7 - 12, 31.03.2019

Yeşim Dağlıoğlu , Huri Özkan Yılmaz Orhan Yılmaz

Abstract

The aim of this study was
to examine the genotoxic effects of ZnOTiO₂ NPs using chromosome
aberration (CA) and micronucleus (MN) tests in human peripheral lymphocytes.
Cells were treated to ZnOTiO₂ NPs concentrations in the range of
12.5 to 125 µg/mL for 24 and 48 hours. In addition, it induced structural CA at all concentrations with ZnOTiO₂
NPs treatment. However, nanoparticles did not stimulate CA in a dose-dependent
manner for 24 and 48 hours of treatment, and there were no statistically
significant differences between both the administration time and the
concentrations (P > 0.05). The
formation of MN induced by ZnOTiO₂ NPs increased depending on
concentration and dose. In particular, the highest 2 concentrations (100 and
125 µg/ml) were observed to be
significantly increased during the 24 and 48 hour treatment period (P >
0.05). Again, there is no significant
difference in the occurrence of MN depending on gender (P > 0.05).

Keywords

Chromosome aberration, ZnOTiO2 NPs, nanoparticles, nanotoxicology, micronucleus

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