Comparative Study of Antagonistic Activity of Scots Pine Root Associated Mycorrhizal Fungus-Bacteria and Wheat Associated Bacteria Against Plant Pathogenic Fungi

Abstract

The aim of the present work was to test antagonistic activity of Scots pine root associated mycorrhizal

fungus-bacteria and wheat associated bacteria against pathogenic fungi Fusarium culmorum, Rhizoctonia

solani, Botrytis cinera and to determine the production of extracellular lytic enzymes by bacterial strains. The

higher number of bacterial strains isolated from wheat showed antagonistic activity compared to bacterial strains

isolated from mycorrhizal hyphae. The best performing strains from mycorrhizal hyphae were Arthrobacter ilicis

KNCL24, Rhodococcus. fascines HNOL8 and from wheat Bacillus cohnii 19, B. subtilis 1, and B. lentus 28. All

isolates presented high level of pectinase activity. Only four strains A. ilicis KNCL24, B. lentus 17, B. subtilis 4, B.

halodurans 12 were able to produce hydrogen cyanide (HCN). Our results showed that bacterial strains associated

with wheat possess more antagonistic activity compared to bacterial strains from mycorrhizal hyphae. The all tested

bacterial strains produced one or more cell wall degrading enzymes.

Keywords

• Mycorrhiza,wheat,antagonistic activity,lytic enzymes

İskoçya Çam Köküyle İlişkili Mikorizal Mantar-Bakteriler ile Buğdayla İlişkili Bakterilerin Patojenik Mantarlara Karşı Antagonistik Etkilerinin Karşılaştırılması

Abstract

Bu çalışmanın amacı, İskoçya çam köküyle ilişkili mikorizal mantar bakterileri ile Fusarium culmorum, Rhizoctonia solani, Botrytis cinera gibi patojenik mantarlara karşı buğdayla ilişkili bakterilerin antagonistik aktivitesini test etmek, ve bakteri suşları ile ekstrasellüler litik enzimlerinin üretimini belirlemektir. Mikorizal hiflerden izole edilen bakteriyel suşlara nazaran, buğdaydan izole edilen yüksek sayıdaki bakteriyel suşlar antagonistik aktivite göstermiştir. Mikorizal hiflerden elde edilen en iyi performansa sahip suşların, Arthrobacter ilicis KNCL24, Rhodococcus. fascines HNOL8 ve buğdaylardan ise Bacillus cohnii 19, B. subtilis 1 ve B. lentus 28 olduğu belirlenmiştir. Tüm izolatlar, yüksek düzeyde pektinaz aktivitesi göstermiştir. Sadece 4 suş (A. ilicis KNCL24, B. lentus 17, B. subtilis 4, B. halodurans 12) hidrojen siyanür üretebilmiştir. Sonuçlarımız, buğdayla ilişkili bakteriyel suşların, mikorizal hiflerden elde edilen bakteriyel suşlardan daha fazla antagonistik aktiviteye sahip olduğunu göstermiştir. Test edilen tüm bakteriyel suşlar, bir yada daha fazla hücre duvarını parçalayan enzimleri üretmiştir

Keywords

• Mikoriza, buğday, antagonistik aktivite, parçalayıcı enzimler

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