Productıon of Foot and Mouth Dısease Vırus Vaccıne (O Type) on Bhk-21 Cell Line

Abstract

Footh & Mouth Disease (FMD) “O” type vaccine was produced on Baby Hamster Kidney (BHK)-

21 cell line. BHK-21 cells were grown in tissue culture flasks. Monolayers were obtained after 48 hours at 37 oC.

Confluent monolayers were infected with FMD “O” type virus (TCID50 104.5/ml). Cytopathic effect (CPE) in the

form of clumping and detachment of the cells was observed after 48 hours. Vaccine was found free of any bacterial

and mycolplasma contamination. Systemic reaction was observed after injecting cattle, mice and guinea pigs with

the virus (TCID50 106/ml). Nine calves of about 6-8 month of age were vaccinated using the same titer used for

safety test and challenged with the virulent virus (TCID50 104.5/ml). All the animals withstood the challenge and did

not show any reaction. Both of the 2 unvaccinated animals after challenge showed typical FMD lesions. Mouth

lesions from ten FMD suspected animals were also collected and adaptation of these field isolates was performed

by passages on BHK-21 cell line. No viral CPE was obtained from these field isolates after 3 passages. Suspected

antigens were also got typed for FMD. The types O (60%), A (20%), and Asia-1 (10%) were found present using

ELISA

Keywords

• BHK,vaccine,challenge,virulent,adaptation,cytopethic effect,antigens

BHK-21 Hücre Hattı üzerinde Şap Hastalığı Virüs Aşısının (O tip) Üretilmesi

Abstract

Şap Hastalığı “0” tipi aşısı, bebek Hamster Böbreğinin (BHK) 21 hücre hattında üretilmiştir. BHK-21 hücreleri doku kültürü şişelerinde dönüştürülmüştür. Otuzyedi 0C’ de 48 saatin sonunda mono katmanlar elde edilmiştir. Birleşen mono katmanlara, Şap hastalığı “O”tipi virüsü bulaştırılmıştır. Hücrelerin kümelenmesı ve ayrılması formunda, Sitopatik etki 48 saat sonra gözlenmiştir. Herhangi bir bakteri ve mikoplazma kontaminasyonu için aşı ücretsiz tedarik edilmiştir. Bu virüse (TCID 106/ml) maruz kalan sığır, fare ve kobaylarda sistematik reaksiyonlar gözlenmiştir. Yaklaşık 6-9 aylık yaşa sahip 9 buzağı, güvenlik testi için kullanılan aynı titrede aşı yapılmış ve öldürücü virüse (TCID 104.5/ml) meydan okunmuştur. Tüm hayvanlar bu zorluğa dayanmış ve herhangi bir reaksiyon göstermemiştir. Bu zorluktan sonra, iki aşılanmayan hayvanın her ikisi de tipik Şap hastalığı lezyonu göstermiştir. Şap hastalığı şüphesi olan 10 hayvandan alınan ağız lezyonları toplandı ve bu alan izolatlarının adaptasyonu, BHK 21. Hücre hattı üzerinden geçişlerle gerçekleştirilmiştir. Üç geçişten sonra bu alan izolatlarından herhangi bir viral sitopatik etki elde edilmemiştir. Şüpheli antijenler de Şap hastalığı için dikkate alınmıştır. ELISA testi ile O (60%), A (20%), ve Asia-1 (10%) tiplerinin varlık gösterdiği belirlenmiştir

Keywords

• Adaptasyon, Antijenler, Aşı, BHK,direnç, virülent, adaptation, Sitopatik etki

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