Yıkama Metodu Kullanılarak Saflaştırılan Amoksisilin Trihidratın Taguchi Yöntemi ile Optimizasyonu

Year 2022, , 933 - 945, 01.06.2022

Mustafa Ergin

https://doi.org/10.21597/jist.1065147

Cited By: 2

Abstract

Saf bir kristal ürün elde etmek için tekrar tekrar uygulanan kristalizasyon basamakları veya farklı kimyasallara başvurulması, bitmiş ürünün veriminde farklı sorunlara neden olabilir. Bundan dolayı, kristal ağın içerisindeki safsızlıklar, ana ürünü etkilemeden uzaklaştırılmalıdır. Bu çalışmada, Amoksisilin trihidrat (Amct) ve safsızlık olarak 4-hidroksifenilglisin (4-HPG) kullanılmıştır. Amct kristal kafesinden, safsızlık olarak 4-HPG’nin yıkama yöntemleri kullanılarak uzaklaştırılması gösterilmiştir. Ayrıca, bu çalışmada ilk kez, yıkama verimi ile yıkama yöntemleri, çözeltiler ve sıcaklıklar arasındaki ilişkiyi incelemek için Taguchi metodu kullanılmıştır. Denemelerde bir Büchner cam hunisi istenilen sıcaklıkta ve ürün kaybı olmadan çalışılacak şekilde yeniden tasarlanmıştır ve denemeler burada yapılmıştır. Bu çalışma, farklı yıkama yöntemleri, kullanılan Taguchi metot ve yeniden tasarlanan Büchner cam hunisi ile literatüre farklılık katmaktadır. Etki grafiklerinden yıkama verim değerlerini etkileyen faktörler sırasıyla yıkama yöntemi, sıcaklık ve çözelti olarak belirlenmiştir. En yüksek verim, tek aşamalı yıkama yöntemi ile Etilalkol kullanılarak 15°C’de elde edilmiştir.

Keywords

Amoksisillin trihidrat, 4-hidroksifenilglisin, kristalizasyon, saflandırma, Taguchi metot

Optimization of Amoxicillin Trihydrate Purified Using Washing Method by Taguchi Method

Abstract

Repeated crystallization operations or the employment of several chemicals to achieve a pure crystalline product might result in a variety of issues with the completed product's yield. As a result, the impurities in the crystal mesh must be eliminated while the core product remains unaffected. Amoxicillin trihydrate (Amct) and 4-hydroxyphenylglycine (4-HPG) were employed as impurities in this investigation. Washing methods were proven to be effective in removing 4-HPG contaminants from the Amct crystal lattice. In addition, for the first time in this study, the Taguchi technique was employed to investigate the link between yield and washing procedures, solutions, and temperatures. A Büchner glass funnel was developed in the experiments to work at the required temperature with no product loss, and testing were undertaken here. This study makes a difference to the literature with different washing methods, the Taguchi method used and the redesigned Büchner glass funnel. From the effect graphs, it was determined that the factors affecting the yield values were washing method, temperature and solution, respectively. The highest washing yield was obtained at 15°C by using ethanol with the one-stage washing method.

Keywords

Amoxicillin trihydrate, 4-hydroxyphenylglycine, crystallization, purification, Taguchi method

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