Uçucu Yağlar İçeren Kitosan Bazlı Filmlerin Antimikrobiyal Aktivitelerinin İncelenmesi

Abstract

Bu çalışmada, kitosan bazlı film üretilerek bu filmlerin yüzey özellikleri incelenmiş ve en uygun formülasyon belirlenmiştir. Film yapısının karakterizasyonu, taramalı elektron mikroskobu, fourier dönüşümlü kızılötesi spektroskopisi, x-ışınları difraksiyonu analizleri yapılarak değerlendirilmiştir. Bu formülasyon farklı konsantrasyonlarda çay ağacı ve\veya melisa uçucu yağı ilavesi yapılarak antimikrobiyal etkinlikleri karşılaştırılmıştır. Filmler mikroyapı açısından değerlendirildiğinde homojen ve esnek bir yüzey yapısı oluşturmuştur. Film yapılarının antimikrobiyal aktivitesi Listeria monocytogenes ATCC 19115, Staphylococcus aureus ATCC 25923, Klebsiella pneumoniae NRRLB 4420, Enterococcus faecalis ATCC 51289, Escherichia coli ATCC 35218, Bacillus subtilis NRS 744, Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 12600, Pseudomonas aeruginosa ATCC 11778 ve Candida albicans ATCC 10231 üzerinde test edilmiştir. Test edilen film yapıları patojen mikroorganizmalar üzerinde antimikrobiyal etki göstermiştir. Uçucu yağların antimikrobiyal aktiviteyi arttırdığı belirlenmiştir.

Keywords

• Antimikrobiyal aktivite
• Kitosan film
• Uçucu yağ

Investigation of Antimicrobial Activities of Chitosan Based Films That Contain Esential Oils

Abstract

In this study, chitosan based films were produced and the most suitable formula in terms of surface properties was determined. The characterization of the film layer was evaluated by scanning electron microscopy, Fourier transform infrared spectroscopy, x-ray diffraction analyses. Different concentrations of tea tree and/or lemon balm essential oil were added to this formula and their antimicrobial activities were compared. When the films were evaluated in terms of microstructure, they formed a homogeneous and flexible surface structure. Antimicrobial activities of films were tested on Listeria monocytogenes ATCC 19115, Staphylococcus aureus ATCC 25923, Klebsiella pneumoniae NRRLB 4420, Enterococcus faecalis ATCC 51289, Escherichia coli ATCC 35218, Bacillus subtilis NRS 744, Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 12600, Pseudomonas aeruginosa ATCC 11778 and Candida albicans ATCC 10231. The tested film structures showed an antimicrobial effect on the pathogenic microorganisms. It has been determined that essential oils increase the antimicrobial activity.

Keywords

• Antimicrobial activity
• Chitosan film
• Esential oil

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