Palynological Investigation of Some Astragalus L. Species Distributed in Şanlıurfa

Year 2024, , 616 - 624, 01.06.2024

Fatma Altın , Hasan Akan , Talip Çeter

https://doi.org/10.21597/jist.1424706

Abstract

In this study, pollen morphologies of 5 species (Astragalus guttatus Banks & Sol., A. cretaceus Boiss., A. caprinus L., A. brachystachys DC. and A. ancistrocarpus Boiss. & Hausskn.) belonging to the Astragalus genus of the family Fabaceae, which are distributed in Şanlıurfa province, were exemined by light and electron microscope. As a result of the study, it was determined that the pollen of the taxa were monad, radial symmetrical and isopolar. Pollen of all studied species are trizonocolporate, colpus length (Clg) 16.5-27.62 µm, colpus width (Clt) 2.15-4.16 µm, pore length (Plg) 6.69-9.32 µm, pore width (Plt) was detected between 6.69-11.44 µm. Polar axis length was measured between 19.23-33.66 µm and equatorial axis diameter was measured as 14.95-28.61 µm. Ornamentation was determined as psilate, psilate-perforate, psilate-microreticulate, microreticulate in the polar region and aperture surround the while microreticulate in the equatorial region. As a result of the study, it was determined that the characters such as pollen shape, pollen size and surface ornamentation differ between species and these characters have taxonmic value in the systematic separation of species.

Keywords

Astragalus, Palinology, Şanlıurfa

Şanlıurfa'da Yayılış Gösteren Bazı Astragalus L. Türlerinin Palinolojik Olarak İncelenmesi

Abstract

Bu çalışmada, Şanlıurfa ilinde yayılış gösteren Fabaceae familyasından Astragalus cinsine ait 5 türün (Astragalus guttatus Banks & Sol., A. cretaceus Boiss., A. caprinus L. subsp. caprinus, A. brachystachys DC. ve A. ancistrocarpus Boiss. & Hausskn.) polen morfolojisi ışık ve elektron mikroskopları ile incelenmiştir. Çalışma sonucunda taksonlara ait polenlerin monad, radyal simetrili ve izopolar oldukları tespit edilmiştir. Çalışılan tüm türlerin polenleri trizonokolporat olup kolpus uzunlukları (Clg)16,5-27,62 µm, kolpus genişlikleri (Clt) 2,15-4,16 µm, por uzunlukları (Plg) 6,69-9,32 µm, por genişlikleri (Plt) 6,69-11,44 µm arasında tespit edilmiştir. Polar eksen uzunlukları 19,23-33,66 µm arasında, ekvatoral eksen çapları 14,95-28,61 µm olarak ölçülmüştür. Ornamentasyon polar bölge ve apertür çevresinde psilat, psilat-perforat, psilat-mikroretikülat ve mikroretikülat olarak saptanırken ekvatoral bölgede mikroretikülat olarak belirlenmiştir. Çalışma sonucunda polen şekli, polen büyüklüğü ve yüzey ornamentasyonu gibi karakterlerin türler arasında farklılık gösterdiği ve türlerin sistematik ayırımında taksonomik değere sahip olabilecek karakterler olduğu tespit edilmiştir.

Keywords

Astragalus, Palinoloji, Şanlıurfa

References

• Akan, H., Tatlıdil, S. & Bıçakçı A. (2005). Pollen morphology of Astragalus L. Section Alopecuroidei DC. (Fabaceae) in Turkey. International Journal of Botany 1(1), 50-58.
• Akan, H., Firat, M. & Ekici, M. (2007). Astragalus bahcesarayensis (Leguminosae-Papilionodeae), a new species of section Alopecuroideio C. FromTurkey. Botanical Journal of the Linnean Society 156, 439-444.
• Al-Ghamadi, F., Osman, A.K.E & Guetat, A. (2013). Contributions to the Pollen Morphology of Genus Astragalus L. (Fabaceae) and its Taxonomic Implications. Asian Journal of Plant Sciences 12, 176-189. doi:10.3923/ajps.2013.176.189.
• Altın F., Çeter T., Çeçen C., & Akan H. (2021). Morphological and Palynological Investigations on Some Taxa of Medicago L. (Fabaceae) Genus Distributing in Şanliurfa. International Journal of Current Naturalscience and Advance Phytochemistry 1 (2), 1-7. https://zenodo.org/record/5809434#.Ycyt9GjP02w.
• Antonio-Domingues, H., Corrêa, A. M. D. S., Queiroz, R. T. D., & Bitar, N. A. B. (2018). Pollen morphology of some Fabaceae species from Patos de Minas, Minas Gerais State, Brazil. Hoehnea, 45, 103-114. https://doi.org/10.1590/2236-8906-54/2017.
• Atasagun, B., Aksoy, A., & Martin, E. (2018). Morphological, Anatomical, Palynological, Karyological and Ecological Remarks of Astragalus argaeus (Fabaceae) Endemic to Turkey. Phytotaxa 379 (1), 118-130. https://doi.org/10.11646/phytotaxa.379.1.10.
• Avcı, S., Sancak, C., Can, A., Acar, A., & Pınar N.M (2013). Pollen Morphology of the Genus Onobrychis (Fabaceae) in Turkey. Turkısh Journal of Botany 37(4), 669-681. doi:10.3906/bot-1207-52.
• Aytaç, Z. & Ekici, M. (2012). Astragalus sp. In: Güner, A., Aslan, S., Ekim, T., Vural, M., Babaç MT (editorler). Türkiye Bitkileri Listesi (Damarlı Bitkiler). İstanbul: Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, pp. 427–456.
• Bagheri, A., Roofigar, A.A., Abbasi, S., Maassoumi, A.A., Rutten, T. & Blatner, F.R., (2019). Pollen morphology of Astragalus section Hymenostegis (Fabaceae) and Evaluation of Its Systematic Implications. Grana 58, 328-336. https://doi.org/10.1080/00173134.2019.1621931.
• Bahadur, S., Taj, S., Long, W. & Hanif, U. (2022). Pollen Morphological Peculiarities of Selected Mimosoideae Taxa of Hainan Island and Their Taxonomic Relevance. Agronomy 12(5), 1122. https://doi.org/ 10.3390/agronomy12051122.
• Chamberlain, D. F. & Mathews, M.A. (1970). Astragalus – In: Davis P. H. (ed.). Flora of Turkey and the east egean İslands, vol 3. Edinburgh University Press, Edinburgh.
• Çeter, T., Karaman, S., Aytaç, Z. & Başer, B (2013a). Pollen morphology of Oxytropis DC. (Fabaceae) genus in Turkey. Bangladesh Journal of Botany 42(1), 167-174.
• Çeter, T., Ekici, M., Pınar, N.M & Özbek, F. (2013b). Pollen Morphology of Astragalus L. Section Hololeuce Bunge (Fabaceae) in Turkey. Acta Botanica Gallica 160(1), 43-52. https://doi.org/10.1080/12538078.2013.791641.
• Dane, F., Aksoy, Ö. D., & Yılmaz, G. (2007). Karyological and Palynological Studies on Astragalus hamosus and A. glycyphyllos in Turkey. Phytologia Balcanica 13(3), 387-391.
• Davis, P.H. & Milli, R.R. (1988). Türkiye florası ve doğu Ege adaları (Ek I). Edinburgh University Press, Edinburgh, 166-169.
• Duman, H., & Akan, H. (2003). New Species of Astragalus (sect. Alopecuroidei: Leguminosae) From Turkey. Botanical Journal of the Linnean Society 143(2), 201-205.
• Ertdman, G. (1969). Handbook of palynology. Morphology, taxonomy and ecology. Copenhagen: Munksgaard.
• Faegri, K., & Iversen, J. (1975). Textbook of pollen analysis. Munksgaard, Copenhagen, 295 pp.
• İşgör, F.G., Alan, Ş., Aşcı, B., Çeter, T., Duran, A. & Pınar, N.M. (2012). Pollen Morphology of the Genus Genista L. (Fabaceae) in Turkey. Mellifera 12(23);12-23.
• Kahraman, A., Binzat, O.K. & Doğan, M. (2013). Pollen Morphology of Some Taxa of Vicia L. subgenus Vicia (Fabaceae) From Turkey. Plant Systematics and Evolution 299(9), 1749-1760.
• Karaman Erkul, S., Çeter, T., & Aytaç, Z. (2017). Türkiye'nin Astragalus L. (Leguminosae) Cinsine Ait Poterion Bunge Seksiyonunun Revizyonu. Ot Sistematik Botanik Dergisi 24(1), 1-20.
• Karaman Erkul, S., Duman, H., & Ateş, M. A. (2022). Astragalus aksutdagensis (Fabaceae), A New Species from Turkey. Nordic Journal of Botany 3, 1-12. https://doi.org/10.1111/njb.03237.
• Khan, A., Ahmad, M., Zafar, M., & Shah, S.A. (2022a). Systematic significance of pollen morphology in rare endemic taxa of Astragalus L. (Fabaceae). Phytotaxa 561 (3), 267-290. DOI: 10.11646/PHYTOTAXA.561.3.4.
• Khan, A., Ahmad, M., Zafar, S., Abbas, Q., Arfan, M., Zafar, M., Sultana, S., Ullah, SA., Khan, S., Akhtar, A., Kilic, O. & Ozdemir, FA. (2022b). Light and scanning electron microscopic observation of palynological characteristics in spineless Astragalus L. (Fabaceae) and Its Taxonomic Significance. Microscopy Research and Technique 85(7), 2409-2427. DOI: 10.1002/jemt.
• Metin, H., Çeter, T., Karaman Erkul, S (2018). Micromorphological characters of pollen, leaflet and seed of Astragalus victoriae and Astragalus melanophrurius endemic to Turkey. Mellifera 18 (1), 22-29.
• Nassiri Semnani, S., Majd, A., Ghassempour, H., Lesani, SN. & Javar, N. (2014). Pollen morphology of the genera Onobrychis (Hedysareae, Fabaceae) in Iran. Journal of Applied Biological Sciences 8(3), 46-51.
• Oskouian, R., Kazempour Osaloo, S., Maasoumi, A.A. (2007). Pollen morphology of Astragalus section Malacothrix and its allies (Fabaceae) in Iran. Journal of Science 32, 177-181.
• Osman, A., Al-Ghamdi, F., & Guetat, A. (2014). Pollen Morphology Of Some Species of Genus Astragalus L.(Fabaceae) in Northern Region of Saudi Arabia. Life Science Journal 11(11), 1006-1019.
• Özbek, F., Ekici, M., Büyükkartal, H.N. & Pınar, N.M. (2021). Anatomy, Palynology and Micromorphology of the Genus Astragalus L.(Fabaceae) Section Uliginosi Gray in Turkey. Journal of the Institute of Science and Technology 11(4), 2525-2536.
• Özhatay, Ö., Kültür, Ş. & Gürdal, B. (2017). Check-list of additinal taxa to the supplement flora of Turkey VIII. Istanbul Journal of Pharmacy 47(1), 30-44. doi: 10.5152/IstanbulJPharm.2017.006.
• Özhatay, Ö., Kültür, Ş. & Gürdal, B (2019). Check-list of additinal taxa to the supplement flora of Turkey IX. Istanbul Journal of Pharmacy 49 (2), 105-120. doi: 10.26650/IstanbulJPharm.2019.19037.
• Pınar. N.M., Akan, H., Çeter, T., Aytaç, Z., Ekici, M., Acar, A., & Akdoğan. S. (2014). Comparative pollen morphology of annual Trigonella L. (Fabaceae) in Turkey. Plant Systematics and Evolution 300(4); 689-708.
• Pinar, M., Ekici, M., Aytac, Z., Akan, H., Ceter, T., & Alan, Ş. (2009). Pollen morphology of Astragalus L. Sect. Onobrychoidei Dc.(Fabaceae) in Turkey. Turkish Journal of Botany 33(4), 291-303.
• Podlech, D. & Zarre, S. (2013). A taxonomic revision of the genus Astragalus L. (Leguminosae) in the Old World, Vols. 1–3. Wien, Naturhistorisches Museum, pp. 2439.
• Podlech, D. (1999). Papilionaceae III, Astragalus I. In: Rechinger KH (ed.) Flora Iranica, Vol. 174. Graz, Austria: Akademische Druck-u. Verlagsanstalt.
• Podlech, D., Zarre, S., [with collaboration of Ekici, M., Maassoumi, A.A. and Sytin, A.] (2013). A taxonomic revision of thegenus Astragalus L. (Leguminosae) in the Old World. Vols. 1-3. Natur historisches Museum Wien, 2439 pp.
• Punt, W., Hoen, P. P., Blackmore, S., Nilsson, S., & Le Thomas, A. (2007). Glossary of pollen and spore terminology. Review of Palaeobotany and Palynology 143(1-2), 1-81.
• Simons, E.P., & Chinnappa, C.C (2004). Pollen Morphology and Taxonomic Status of North American Astragalus and Oxytropis(Papilionoideae: Fabaceae). Beitrage zur Biologie der Pflanzen 73, 307- 319.
• Wodehouse, R. P. (1935). Pollen grains: their structure, identification and significance in science and medicine. McGraw-Hill Book Company, New York.
• Wojciechowski, M.F., Lavin, M. & Sanderson, M.J. (2004). A phylogeny of Legumes (Leguminosae) based on analysis of the plastid MATK gene resolves many well-supported subclades within the family. American Journal of Botany 91(11),1846-2.