Resveratrol Attenuates Cisplatin-Induced Oxidative Stress: Combined Effects in MDA-MB-231 Breast Cancer Cells

Abstract

Cisplatin, widely preferred in cancer treatment, is known to inhibit neoplastic cell proliferation while simultaneously causing harmful effects in normal cells. Various research has indicated that cisplatin-induced cytotoxicity is closely correlated with an elevated generation of reactive oxygen species (ROS). Antioxidants are significant defense mechanisms in biological systems that neutralize damage induced by ROS, thereby protecting cells against the adverse effects of carcinogens, toxic radical reactions, and pharmacological agents. In this research, resveratrol, known for its numerous biological properties such as anti-inflammatory, antioxidant, and anti-tumor effects, has been used in combination with cisplatin. Specifically, the potential protective effects of resveratrol against cisplatin-induced oxidative stress damage in MDA-MB-231 breast cancer cells were evaluated. In this context, the levels of total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione (GSH) were determined both separately and in combination with cisplatin and resveratrol. When compared to the control group, TAS, SOD, and GSH levels were found to be lower in cells treated with cisplatin, while an increase in MDA and TOS levels was observed. However, when cisplatin and resveratrol were combined, there was a statistically significant increase in TAS, SOD, and GSH levels, and a decrease in TOS and MDA levels compared to the group treated with cisplatin alone. The findings indicate that cisplatin triggers oxidative stress in MDA-MB-231 cells by reducing the levels of antioxidant enzymes and increasing lipid peroxidation. Additionally, it was observed that this oxidative damage significantly decreased when combined treatment with resveratrol was applied.

Keywords

• Oxidative stress
• breast cancer
• cisplatin
• resveratol

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