Determination Of Cytotoxicity Induced By 1,4 Dioxane In Root Tip Cells Of Allium Cepa L. (Amaryllıdaceae)

Abstract

In this study physiological and cytogenetic effects of 1,4 Dioxane used in daily lives frequently in the composition of cleaning and cosmetic products on Allium cepa L. root tip cells were investigated. A.cepa seeds were used as test material. Germination percentage, root lenght and weight gain was used as physiological indicators and chromosomal damage, micronucleus (MN) frequency, mitotoc index (MI) was used as cytotoxicity indicators and these datas were correlated with statistical parameters. The seeds of A.cepa were divided into three groups: control (Group I) and 1,4 Dioxane treatment groups. Group II and Group III were treated with 50 ppm and 100 ppm 1,4 Dioxane, respectively for 72 hours. As a result, it was determined that Dioxane fairly decreased the germination percentage, root length and weight gain depending on dose in seeds all treatment groups, whereas chromosomal damage and MN rate was increased. In conclusion, data obtained in this study indicated that 1,4 Dioxane has a dose dependent cytotoxic effects on root tip cells of A.cepa.

Keywords

• 1,4 Dioxane,cytotoxicity,chromosomal damage,micronucleus,seed germination,Allium cepa L.

Allium Cepa L. (Amaryllıdaceae) Kök Ucu Hücrelerinde 1,4 Dioksan Tarafından Teşvik Edilen Sitotoksisitenin Belirlenmesi

Abstract

Bu çalışmada günlük yaşamımızda sıkça kullandığımız temizlik ve kozmetik ürünlerinin yapısında bulunan, 1,4 Dioksanın Allium cepa L. kök ucu hücrelerinde meydana getirdiği zyolojik ve sitogenetik etkiler araştırılmıştır. Test materyali olarak A.cepa tohumları kullanılmıştır. Çimlenme yüzdesi, kök uzunluğu ve ağırlık artışı zyolojik parametreler olarak; kromozomal hasarlar, mikronukleus (MN) sıklığı ve mitotik indeks (MI) ise sitotoksisitenin indikatörleri olarak kullanılmış ve bu veriler istatistiksel parametreler ile ilişkilendirilmiştir. A.cepa tohumları kontrol (Grup I) ve 1,4 Dioksan uygulama grupları olarak üç gruba ayrıldı.72 saat süresince II. Gruba 50 ppm 1,4 Dioksan, III. Gruba 100 ppm dozunda 1,4 Dioksan uygulanmıştır. Sonuçta, 1,4 Dioksanın tüm uygulama gruplarında doza bağlı olarak çimlenme yüzdesi, kök uzunluğu ve ağırlık kazanımını azalttığı, kromozomal anormallikler ve MN oranını ise arttırdığı tespit edilmiştir. Sonuç olarak, elde edilen veriler 1,4 Dioksanın A.cepa kök ucu hücreleri üzerinde doza bağlı sitotoksik bir etkiye sahip olduğunu göstermiştir.

Keywords

• 1,4 Dioksan,sitotoksisite,kromozomal hasarlar,mikronukleus,tohum çimlenmesi,Allium cepa L.

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