The Effects of BAP with NAA and IBA on Microtuberization of in vitro Grown Potatoes (Solanum tuberosum L.) under Different Photoperiod Conditions

Abstract

The present research was carried out to determine the effect of benzyl aminopurine (BAP) with α-naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA) combinations on the microtuberization of Pasinler, Granola and Caspar potato genotypes using stem segments with single nodes. Explants were incubated at two different photoperiod conditions such as short day (8 hours daylight) and continuous dark. Observations from the microtuber characteristics were examined in this article. Microtuberization started earlier under continiuous dark (50.98 days) than short days (55.88 days) conditions. Higher number of microtubers per plantlets was determined on continuous dark (2.94) than 8 hours photoperiod (2.35). Total microtuber weight was also higher under continuous dark (432.02 mg) than short day (389.13 mg) although differences between them were insigni cant. Presented results showed that the effect of continuous dark was more dominant than short days and the in uence of control treatment including only 8%sucrose and combination of BAP+IBA was more pronounced compared to other hormonal treatments. Findings of the research also revealed that the effect of PGRs on plantlet characteristics studied was variable depending on the genotype. 

Keywords

• Potato,biotechnology,tissue culture,in vitro,microtuber

Farklı Fotoperiyot Şartlarında in vitro Olarak Yetiştirilen patates (Solanum tuberosum L.)’lerde BAP’ın NAA ve IBA ile Birlikte Mikro Yumru Oluşturma Üzerine Etkileri

Abstract

Bu çalışma, benzyl aminopurine (BAP)’in tek başına ya da α-naphthaleneacetic acid (NAA) ve indole-3-butyric acid (IBA) ile birlikte Pasinler, Granola ve Caspar patates çeşitlerinin tek boğum sürgün kesimlerinin mikroyumru (MY) oluşturması üzerine etkisini belirlemek için yürütülmüştür. Eksplantlar kısa gün şartları (8 saat ışık) ve tamamen karanlık şartları gibi iki farklı fotoperiyot şartlarında gelişmeye bırakılmışlardır. Bu makalede patateste MY oluşumu özelliklerinden alınan gözlemler değerlendirilmiştir. MY oluşumu tamamen karanlık şartlarda (50.98 gün) kısa gün şartlarına gore (55.88 gün) daha erken başlamıştır. Karanlık şartlarda MY sayısı (2.94 adet) 8 saat ışık şartlarına göre (2.35 adet) daha fazla elde edilmiştir. Aradaki fark istatistiki olarak önemli olmamasına rağmen, toplam MY ağırlığının da karanlık şartlarda (432.02 mg), kısa gün şartlarından (389.13 mg) daha fazla olduğu görülmüştür. Hiç hormon içermeyen MS ortamı (kontrol) en yüksek MY değerleri vermiştir. Elde edilen sonuçlar, tamamen karanlık şartların kısa gün şartlarına göre, %8 sukroz içeren hormonsuz kontrol ortamının diğer bitki büyüme düzenleyicilerine göre daha üstün olduğunu, BAP+IBA uygulamasının kontrolden sonraki en uygun uygulama olduğunu göstermiştir. Bulgular ayrıca, kullanılan bitki büyüme düzenleyicilerinin etkisinin çeşide bağlı olarak değişebileceğini göstermiştir.

Keywords

• Patates,bioteknoloji,doku kültürü,in vitro,mikroyumru

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