Physio-Biochemical Characterization and 16S rRNA Sequencing of Thermophilic Geobacillus sp. stain DB2 from Dibekli Hot Spring, Turkey

Abstract

Geothermal sites are habitat for thermophilic microorganisms. In this study, a novel aerobic, rod
shaped, motile and Gram-positive thermophilic bacterium DB2 was isolated from the hot water in Dibekli in
the town of Diyadin in Turkey and it was identifed by morphological, physiological, biochemical tests and 16S
rRNA gene sequencing. Optimal cultivation was obtained at pH 6.0–6.5 and 60 oC. Strain DB2 was positive for
catalase activity, nitrate reduction, Methyl red test and starch hydrolysis, while it was negative for oxidase, urease,
gelatine and casein hydrolysis. Phylogenetic analysis based on 16S rRNA gene sequences showed that isolate DB2
was most closely related to Geobacillus subterraneus subsp. aromaticivorans Ge1(T) with a 98.97% sequence
similarity. This novel strain can be used in biotechnological application.

Keywords

• 16S rRNA,Geobacillus,identifcation,thermophilic

Türkiye’deki Dibekli Sıcak Su Kaynağından Termoflik Geobacillus sp. DB2 Suşunun Fizyo-Biyokimyasal Karakterizasyonu ve 16S rRNA Sekanslaması

Abstract

Jeotermal kaynaklar termofllerin habitatlarındandır. Bu çalışmada aerobik, çubuk şeklinde, hareketli
ve Gram-pozitif termoflik DB2 suşu Türkiye’deki Diyadin ilçesi Dibekli sıcak su kaynağından izole edilerek
morfolojik, fzyolojik, biyokimyasal testler ve 16S rRNA gen sekanslamayla tanımlandı. Optimal kültivasyon
pH 6.0–6.5’da, 60 oC’de elde edildi. DB2 suşunun katalaz, nitrat redükleme, Metil red testi ve nişasta hidrolizi
pozitif, oksidaz, üreaz aktivitesi, jelatin ve kazein hidrolizi negatif olarak belirlendi. 16S rRNA gen sekansına bağlı
flogenetik analizler DB2 suşunun %98.97 sekans benzerliği ile Geobacillus subterraneus subsp. aromaticivorans
Ge1(T)’e oldukça yakın olduğunu göstermektedir. Bu yeni tür, biyoteknolojik uygulamalarda kullanılabilir
özelliktedir

Keywords

• 16S rRNA geni,Geobacillus,tanımlama,termoflik

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