Investigation of changes on some morphological characteristics in different eggplant scion/rootstock combinations under salt and drought stresses.

Abstract

In this study, eggplants grown with both grafted, salt tolerant rootstocks and non-grafted roots from
four different eggplant genotypes, under salt stress and drought inducing conditions, were investigated during
their pre-flowering stages. For the plant materials, four eggplant scion (salt tolerant: Mardin Kızıltepe and Burdur
Merkez; salt sensitive: Artvin Hopa and Kemer) and two rootstock (Köksal- F1, Vista-306) varieties were used.
The plants in 12 different grafting combinations had salt and drought conditions applied to each of them. The
drought stress (irrigated when 50% of available moisture was depleted) and the salt stress (6 dSm-1) were applied
individually. At the end of the applied stress conditions, the plant samples were evaluated according to fresh weight
and dry weight of their green parts and roots, leaf area, stem diameter, shoot and root heights. In this study, the
highest fresh weight and dry weight were determinated in Köksal F1/Kemer (284.42 and 50.59 g plant-1). Moreover,
the least changes in root dry weight was observed in Köksal-F1/Burdur Merkez (11.40% ) under drought and
Köksal-F1/Artvin Hopa (5.71 %) under salinity. These results indicated that grafted plants were more resistant to
stress conditions than non-grafted plants. Overall, it was determined that Köksal-F1 rootstock had a more effective
tolerance to salt and drought stress conditions than the Vista rootstock variety

Keywords

• Grafting,plant biomass,drought,salinity,Solanum melongena

Tuzluluk ve Kuraklık Stresi Altında Farklı Patlıcan Anaç/Kalem Kombinasyonlarının Bazı Morfolojik Özelliklerinde Meydana Gelen Değişimlerin İncelenmesi

Abstract

Bu çalışmada; tuza toleransı yüksek 2 ticari patlıcan anacı üzerine aşılanan ve aşısız olarak kendi
kökleri üzerinde yetiştirilen 4 patlıcan genotipinin gelişme performansları, kuraklık ve tuz stresi koşulları altında
çiçeklenme öncesindeki bitki döneminde incelenmiştir. Bitkisel materyal olarak 4 patlıcan kalem genotipi (tuza
tolerant: Mardin Kızıltepe, Burdur Merkez; tuza duyarlı: Artvin Hopa ve Kemer), 2 adet patlıcan anacı (Köksal-F1,
Vista-306) kullanılmıştır. Toplam 12 farklı uygulamadaki bitkilere kuraklık ve tuzluluk uygulamaları ayrı ayrı
yapılmış ve değerlendirilmiştir. Kuraklık stresi yarayışlı su seviyesi %50 düzeyinde tutularak; tuz stresi ise 6 dSm-1
tuz seviyesinde sulama suyu uygulanarak oluşturulmuştur. Stres sonunda alınan bitki örnekleri; yeşil aksam yaş ve
kuru ağırlığı, kök yaş ve kuru ağırlığı, yaprak alanı, gövde çapı, gövde ve kök boyları bakımından incelenmiştir.
Çalışmada, yeşil aksam yaş ve kuru ağırlık değerleri en yüksek olan kombinasyon Köksal F1/Kemer (284.42
ve 50.59 g bitki-1) olarak belirlenirken, kuraklık stresi karşısında kök kuru ağırlığı %11.40 ile Köksal-F1/Burdur
Merkez’de, tuz stresi altında ise %5.71 değeri ile Köksal-F1/Artvin Hopa’da en az değişim gerçekleşmiştir
İncelenen özellikler bakımından aşılı bitkilerin kuraklık ve tuz stresi karşısında aşısızlara göre daha az kayıplar
verdiği, Köksal-F1 anacı üzerine aşılamanın kuraklık ve tuz stresine dayanımda, Vista anacına göre daha etkili
olduğu sonucuna varılmıştır

Keywords

• Aşılama,bitki biyoması,kuraklık,tuzluluk,Solanum melongena

References

1. Abdalla MM, El-Khoshiban NH, 2007. The influence of water stress on growth, relative water content, photosynthetic pigments, some metabolic and hormonal contents of two Triticium aestivum cultivars. Journal of Applied Sciences Research, 3(12): 2062-2074.
2. Abdelmageed, AH, Gruda N, Geyer B, 2004. Effects of temperature and grafting on grwth and development of tomato plants under controlled conditions. Deutscher Tropentag 2004, Berlin. http://www.tropentag.de/2004/abstracts/full/106.pdf. (Erişim tarihi: 05 Ağustos, 2016).
3. Altunlu H, 2011. Aşılamanın Domateste Kuraklık Stresine Etkileri. Ege Üniversitesi Fen Bilimleri Enstitüsü, Doktora Tezi, İzmir, 204s.
4. Anonim 2015. Food and Agricultural Organization FAO.http://faostat3.fao.org/browse/Q/C/E. Erişim Tarihi: 11.8.2016.
5. Anonymous 2016. Mechanisms of salt tolerance. http://agritech.tnau.ac.in./agriculture/ agri_salinity_tolerance.html (Erişim tarihi: 24.12.2015)
6. Asraf M, Arfan M, Ahmad A, 2003. Salt tolerance in okra: Ion relations and gas exchanges characteristics. Journal of Plant Nutrition, 26 (1): 63-79.
7. Asraf M, Iram A, 2005. Drought stress induced changes in some organic substances in nodules and other plant parts of two potential legumes differing in salt tolerance. Flora, 200: 535–546.
8. Bertamini M, Zulini L, Muthuchelian K, Nedunchezhian N, 2006. Effect of water deficit on photosynthetic and other physiological responses in grapevine (Vitis vinifera L. cv. Riesling) plants. Photosynthetica, 44 (1): 151- 154.

9. Canizares KAL, Goto R, Vilas BRL, 2000. Yield and nutrient content in Japanese cucumber grafted on squash. Horticultura Argentina, 19(47): 5-10.
10. Chartzoulakis K, Klapaki G, 2000. Response of two greenhouse pepper hybrids to NaCl salinity during different growth stages. Scientia Horticulture, 86: 247-260.
11. Çetin F, Fricke A, Stützel H, 2009. Interactions between tomato scion and rootstock varieties regarding growth and development under different water supply levels. Tropentag 2009, Biophysical and socio-economic frame conditions for the sustainable management of natural resources, October 6 - 8, Hamburg, Germany. Book of Abstracts, p:176, ID 955.
12. Daşgan HY, Aktaş H, Abak K, Çakmak I, 2002. Determination of screening techniques to salinity tolerance in tomatoes and investigation of genotype responses. Plant Science, 163: 695-703.
13. Daunay MC, Lester RN, Ano G, 2001. Eggplant. in:Tropical Plant Breeding (Eds: Charrier A, Jaquot M, Hamon S, Nicolas D). Science Publishers, Inc. Enfield (NH), USA, UK, pp:199-222.
14. Davis AR, Perkins-Veazie P, Sakata Y, López-Galarza S, Maroto JV, Lee SG, Huh YC, Sun Z, Miguel A, King Cohen R, Lee J, 2008. Cucurbit grafting. Critical Reviews in Plant Sciences, 27: 50–74.
15. De Pascale S, Barbieri G, 1997. Effect of soil salinity and top removal on growth and yield of broadbean as a green vegetable. Sci. Hort. 71:147-165.
16. Eisa S, Hussin S, Geissler N, Koyro HW, 2012. Effect of NaCl salinity on water relations, photosynthesis and chemical composition of quinoa (Chenopodium quinea Wild.) as a potential cash crop halophyte. AJCS, 6(2): 357-368.
17. El-Shraiy A, Mostafa MA, Zaghlool SA, Shehata SAM, 2011. Alleviation of salt injury of cucumber plant by grafting onto salt tolerance rootstock. Australian Journal of Basic and Applied Sciences, 5(10), 1414-1423.
18. Freed R, Einensmith SP, Guets S, Reicosky D, Smail VW, Wolberg P, 1989. User’s guide to MSTAT-C, an analysis of agronomic research experiment. Michigan State University, USA.
19. He Y, Zhu Z, Yang J, Ni X , Zhu B, 2009. Grafting increases the salt tolerance of tomato by improvement of photosynthesis and enhancement of antioxidant enzymes activity. Environmental and Experimental Botany, 66: 270-278.
20. Huang Y, Zhilong B, Sanpeng H, Hua B, Zhen A, Zhixiong L, 2010. Improving cucumber tolerance to major nutrients induced salinity by grafting onto Cucurbita ficifilia. Environmental and Experimental Botany, 69:32-38.
21. Huang Y, Bie ZL, Liu ZX, Zhen A, Jiao XR, 2011. Improving cucumber photosynthetic capacity under NaCl stress by grafting onto two salt-tolerant pumpkin rootstocks. Biologia Plantarum, 55(2): 285-290.
22. Gluffrida F, Martorana M, Leonardi C, 2009. How sodium chloride concentration in the nutrient solution influences the mineral composition of tomato leaves and fruit. HortScience, 44(3): 707-711.
23. Karanlık S, 2001. Değişik Buğday Genotiplerinde Tuz Stresine Dayanıklılık veDayanıklılığın Fizyolojik Nedenlerinin Araştırılması. Çukurova Üniversitesi FenBilimleri. Enstitüsü. Doktora Tezi, Adana 123 s.
24. Kıran S, Özkay F, Ellialtıoğlu Ş, Kuşvuran Ş, 2014. Tuz Stresine Tolerans Seviyeleri Belirlenmiş Bazı Genotiplerin Kuraklık Stresine Tepkilerinin Belirlenmesi. Tagem Sonuç Raporu. Proje No: A-02.P-04, Ankara.
25. Kıran S, Kuşvuran Ş, Özkay F, Özgün Ö, Sönmez K, Özbek H, Ellialtıoğlu ŞŞ, 2015. Bazı patlıcan anaçlarının tuzluluk stresi koşullarındaki gelişmelerinin karşılaştırılması. Tarım Bilimleri Araştırma Dergisi, 8(1): 20-30.
26. Köksal E, İlbeyi A, Üstün H, Özcan H, 2007. Yeşil Fasulye Sulama Suyu Yönetiminde Örtü Sıcaklığı ve Spektral Yansıma Oranı Değerlerinin Kullanım Olanakları. TAGEM Yayın No: TAGEM-BB-TOPRAKSU-29. 26s.
27. Kurum R, Ulukapı K, Aydınşakir K, Onus NA, 2013. The influence of salinity on seedling growth of some pumpkin varieties used as rootstock. Not Bot Horti Agrobo, 41(1):219-225.
28. Kuşvuran Ş, Yaşar F, Abak K, Ellialtıoğlu Ş, 2008. Tuz stresi altında yetiştirilen tuza tolerant ve duyarlı Cucumis sp.’nin bazı genotiplerinde lipid peroksidasyonu, klorofil ve iyon miktarlarında meydana gelen değişimler. Journal of Agricultural Science, 18(1): 11-18.
29. Liu ZX, Bie ZL, Huang Y, Zhen A, Lei B, Zhang HY, 2012. Grafting onto Cucurbita moschata rootstock alleviates salt stress in cucumber plants by delaying photoinhibition. Photosynthetica, 50(1): 152-160.
30. Lopez H, Marco A, Ulery AP, Zohrab S, Picchioni G, Flynn RP, 2011. Response of chile pepper (Capsicum annuum L.) to salt stress and organic and inorganic nitrogen sources: I Growth and yield. Tropical and Subtropical Agroecosystems, 14(1), 137-147.
31. Munns R, Tester M, 2008. Mechanisms of salinity tolerance. Annu. Rev. Plant Biol., 59: 651-681.
32. Özmen S, Kanber R, Sarı N, Ünlü M, 2014. Damla sulama koşullarında aşılı ve aşısız karpuzlarda bitki, su ve verim ilişkilerinin irdelenmesi. Düzce Üniversitesi Bilim ve Teknoloji Dergisi, 2: 141–153
33. Öztekin GB, 2009. Aşılı Domates Bitkilerinde Tuz Stresine Karşı Anaçların Etkisi. Ege Üniversitesi Fen Bilimleri Enstitüsü, Doktora Tezi, 338s.
34. Öztekin GB, Tüzel Y, 2011. Salinity response of some tomato rootstocks at seedling stage. African Journal of Agricultural Research, 6(20): 4726-4735.
35. Pimmongkol A, Terapongtanakhon S, Udonsirichakhon K, 2002. Anatomy of salt-and non-salt-tolerant rice treated with NaCl. In: 28th Congr. Science and Technology of Thailand, Bangkok, Thailand.
36. Rivero RM, Ruiz JM, Sanchez E, Romero L, 2003. Does grafting provide tomato plants an advantage against H2O2 production under conditions of thermal shock. Physiologia Plantarum, 117: 44-50.
37. Razi H, Asad MT, 1998. Evaluation of variation of agronomic traits and water stress tolerant in sunflower conditions. Agricultural and Natural Resources Sciences, 2: 31-43.
38. Savvas D, Colla G, Rouphael Y, Schwarz D, 2010. Amelioration of heavy metal and nutrient stress in fruit vegetables by grafting. Scientia Horticulturae, 127: 156-161.
39. Solmaz I, Sarı N, Dasgan Y, Aktaş H, Yetişir H, Ünlü H, 2011. The effect of salinity on stomata and leaf characteristics of dihaploid melon lines and their hybrids. Journal of Food, Agriculture and Environment, 9(3&4): 172-176.
40. Talhouni M, 2016. Patlıcanda Tuzluluk Stresine Dayanımın Artırılmasında Anaçların ve Yerel Gen Kaynaklarının Etkinliği Üzerinde Araştırmalar. Ankara Üniversitesi, Fen Bilimleri Enstitüsü, Bahçe Bitkileri Anabilim Dalı, Doktora Tezi, Ankara, 199 s.
41. Tıpırdamaz R, Ellialtıoğlu Ş, 1994. Domates Genotiplerinde Tuza Dayanıklılığın Belirlenmesinde Değişik Tekniklerin Kullanımı. A.Ü. Ziraat Fakültesi Yayınları, Yayın No: 1358, Bilimsel Araştırma ve İncelemeler: 752, 21s.
42. Türkan İ, Bor M, Özdemir F, Koca H, 2005. Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought sensitive P. vulgaris L. subjected to polyethylene glycol mediates water stress. Plant Science, 168: 223-231.
43. Voutsela S, Yarsi G, Petropoulos SA, Khan EM, 2012. The effect of grafting of five different rootstocks on plant growth and yield of tomato plants cultivated outdoors and indoors under salinity stress. Afr. J. Agric. Res., 7 (41): 5553–5557
44. Yaşar F, 2003. Tuz Stresi Altındaki Patlıcan Genotiplerinde Bazı Antioksidant Enzim Aktivitelerinin in vitro ve in vivo Olarak İncelenmesi. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü, Doktora Tezi, Van, 139s.
45. Yetişir H, Uygur V, 2009. Plant growth and mineral element content of different gourd species and watermelon under salinity stress. Turkish Journal of Agriculture and Forestry, 33: 65-77.
46. Zeng YA, Zhu YL, Huang BJ, Yang LF, 2004. Effects of Cucurbita ficifolia as rootstock on the growth, fruit set, disease resistance and leaf nutrient content of Cucumis sativus. Journal of Plant Resources and Environment. Institute of Botany, Jiangsu Province and the Chinese Academy of Sciences, Nanjing, China, 13(4), 15-19.
47. Zhu J, Bie Z, Huang Y, Han X, 2008. Effect of grafting on the growth and ion concentrations of cucumber seedlings under NaCl stress. Soil Science and Plant Nutrition. 54, 895-902.