Determination Of Prokaryotic Diversity Of Salda Lake By Next Generation Sequencing Method

Year 2017, Volume: 7 Issue: 3, 99 - 106, 30.09.2017

Nilgün Poyraz , Mehmet Burçin Mutlu

Abstract

In our study, water sample was taken from Salda Lake and the pH and salinity data for the sample
were determined. After that, samples were fltered and DNA extraction was performed. The obtained DNA was
sequenced by the Illumina MiSeq sequencing system and data was analyzed by the QIIME program. When the
data obtained after the analysis were evaluated, it was determined that the most dominant bacterial phyla are
Proteobacteria and Actinobacteria. At class level, Gammaproteobacteria, Acidimicrobia and Actinobacteria were
dominant. In order level Enterobacteriales, Acidimicrobiales, Actinobacteriales were abundant. When the Archaea
domain was evaluated it was determined that the Euryarchaeota phylum was dominant. Halobacteria members
were abundant at the class level. In addition Halobacteria class, Halobacteriales order, Halobacteriaceae family
includes Haloarcula, Halobacterium, Halonotius, Halorhabdus, Halorubrum and Haloplanus genus members

Keywords

Alkaliphilic , prokaryotic diversity , Salda Lake , next generation sequencing

Salda Gölü Prokaryotik Çeşitliliğinin Yeni Nesil Dizileme Yöntemiyle Belirlenmesi

Abstract

Çalışmada Salda Gölü’nden örnekleme yapılmış ve örnek için pH ve tuzluluk değerleri belirlenmiştir.
Sonrasında örnekler fltreden geçirilerek, bu fltrelerden DNA ekstraksiyonu işlemi yapılmıştır. Elde edilen DNA
Illumina MiSeq dizileme sistemiyle dizilenmiş ve veriler QIIME Programı ile analiz edilmiştir. Analiz sonrası elde
edilen veriler değerlendirildiğinde en baskın bulunan Bacteria flumlarının Proteobacteria ve Actinobacteria olduğu
belirlenmiştir. Sınıf düzeyinde ise Gammaproteobacteria, Acidimicrobia ve Actinobacteria baskındır. Ordo düzeyinde
ise Enterobacteriales, Acidimicrobiales, Actinobacteriales baskındır. Archaea domaini değerlendirildiğinde ise
Euryarchaeota flumunun baskın olduğu belirlenmiştir. Sınıf düzeyinde Halobacteria üyeleri baskındır. Halobacteria
sınıfının Halobacterialaes ordosu Halobacteriaceae familyasının Haloarcula, Halobacterium, Halonotius,
Halorhabdus, Halorubrum ve Haloplanus cinsi üyeleri de baskın olarak bulunmaktadır

Keywords

Alkaliflik , prokaryotik çeşitlilik , Salda Gölü , yeni nesil dizileme

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