Investigation of Inhibition Effect of Oxytocin on Carbonic Anhydrase and Acetylcholinesterase Enzymes in the Heart Tissues of Rats

Abstract

In our study, it was aimed to investigate the effects of oxytocin, oxytocin receptor antagonist

atosiban and oxytocin-atosiban injected to rats on carbonic anhydrase (CA) and acetylcholinesterase (AChE)

enzyme activities in the heart of rats. For this purpose, four different groups (n = 6) consisting of a total of 24

rats, were formed. (Control group, oxytocin treated group, atosiban treated group, and oxytocin and atosiban

combination treated group). The rats were necropsied after 60 minute intraperitoneal injection of the chemicals into

the rats. Cardiac tissue of rats was removed. The enzyme activity averages of the groups are statistically compared.

According to this, while the activity of carbonic anhydrase enzyme in heart tissue of rats decreases in oxytocin

and atosiban given groups, in the combination group with oxytocin and atosiban it was seen that the reduction

was relatively less according to the others. Acetylcholinesterase activity decreases in both groups of oxytocin and

atosiban which given groups did not show any significant change in the group in which oxytocin and atosiban were

given together.

Keywords

• enzyme activity,carbonic anhydrase,oxytocin,rat

Sıçanların Kalp Dokusunda Oksitosin’in Karbonik Anhidraz ve Asetilkolinesteraz Enzimleri Üzerine İnhibisyon Etkisinin Araştırılması

Abstract

Çalışmamızda, sıçanlara enjekte edilen oksitosin, oksitosin reseptör antagonisti atosiban ve oksitosinatosiban

hormonlarının, sıçanların kalp dokusunda karbonik anhidraz (CA) ve asetilkolinesteraz (AChE) enzim

aktivitelerine etkilerinin incelenmesi amaçlanmıştır.Bu amaçla, toplam 24 adet sıçandan oluşan dört farklı grup

(n=6) oluşturuldu. (Kontrol grubu, oksitosin verilen grup, atosiban verilen grup ve oksitosin ile atosiban’ın birlikte

verildiği grup). Sıçanlara kimyasallar intraperitoneal olarak enjekte edildikten 60 dakika sonra sıçanlar nekropsiye

alındı. Sıçanların kalp dokuları çıkarıldı. Elde edilen her bir enzim için aktivite değerleri istatistiksel olarak

hesaplandı.

Buna göre sıçanların kalp dokusunda karbonik anhidraz enzim aktivitesi oksitosin ve atosiban verilen gruplarda

azalışa neden olurken, oksitosin ile atosibanın birlikte verildiği grupta ise azalmanın diğerlerine nispeten daha az

olduğu görüldü. Oksitosin ve atosiban verilen gruplarda asetilkolinesteraz aktivitesinde görülen azalma, oksitosin

ve atosiban’ın birlikte verildiği grupta gözlenmedi.

Keywords

• enzim aktivitesi,karbonik anhidraz,oksitosin,rat

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